Cellosaurus HEC-LTT (CVCL_A7FW)
| Cell line name | HEC-LTT |
|---|---|
| Synonyms | HUVEC Uni-TAg/bi-hTert |
| Accession | CVCL_A7FW |
| Resource Identification Initiative | To cite this cell line use: HEC-LTT (RRID:CVCL_A7FW) |
| Comments | Characteristics: Transduced with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Characteristics: In the presence of doxycycline, hTERT and SV40-TAg expression are activated, resulting in high cell proliferation and unlimited expansion. In the absence of the doxycycline, cells stop proliferating and can be maintained in a growth-arrested state that reflects the status of endothelial cells in vivo (PubMed=26345505). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Umbilical vein; UBERON=UBERON_0002066. Cell type: Endothelial cell of umbilical vein; CL=CL_0002618. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Sex of cell | Sex unspecified |
| Age at sampling | <1D |
| Category | Conditionally immortalized cell line |
| Publications | PubMed=19705962; DOI=10.1089/ten.TEA.2009.0184 PubMed=26345505; DOI=10.2144/000114326 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107115253 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 14-Aug-2025 |
| Version number | 11 |