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Cellosaurus HEC-LTT (CVCL_A7FW)

[Text version]
Cell line name HEC-LTT
Synonyms HUVEC Uni-TAg/bi-hTert
Accession CVCL_A7FW
Resource Identification Initiative To cite this cell line use: HEC-LTT (RRID:CVCL_A7FW)
Comments Characteristics: Transduced with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Characteristics: In the presence of doxycycline, hTERT and SV40-TAg expression are activated, resulting in high cell proliferation and unlimited expansion. In the absence of the doxycycline, cells stop proliferating and can be maintained in a growth-arrested state that reflects the status of endothelial cells in vivo (PubMed=26345505).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Umbilical vein; UBERON=UBERON_0002066.
Cell type: Endothelial cell of umbilical vein; CL=CL_0002618.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Sex unspecified
Age at sampling <1D
Category Conditionally immortalized cell line
Publications

PubMed=19705962; DOI=10.1089/ten.TEA.2009.0184
May T., Butueva M., Bantner S., Markusic D., Seppen J., MacLeod R.A.F., Weich H., Hauser H., Wirth D.
Synthetic gene regulation circuits for control of cell expansion.
Tissue Eng. Part A 16:441-452(2010)

PubMed=26345505; DOI=10.2144/000114326
Lieber D., Hochdorfer D., Stoehr D., Schubert A., Lotfi R., May T., Wirth D., Sinzger C.
A permanently growing human endothelial cell line supports productive infection with human cytomegalovirus under conditional cell growth arrest.
BioTechniques 59:127-136(2015)

Cross-references
Encyclopedic resources Wikidata; Q107115253
Entry history
Entry creation20-May-2021
Last entry update10-Apr-2025
Version number10