ID   HEC-LTT
AC   CVCL_A7FW
SY   HUVEC Uni-TAg/bi-hTert
DR   Wikidata; Q107115253
RX   PubMed=19705962;
RX   PubMed=26345505;
CC   Characteristics: Transduced with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
CC   Characteristics: In the presence of doxycycline, hTERT and SV40-TAg expression are activated, resulting in high cell proliferation and unlimited expansion. In the absence of the doxycycline, cells stop proliferating and can be maintained in a growth-arrested state that reflects the status of endothelial cells in vivo (PubMed=26345505).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Umbilical vein; UBERON=UBERON_0002066.
CC   Cell type: Endothelial cell of umbilical vein; CL=CL_0002618.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Sex unspecified
AG   <1D
CA   Conditionally immortalized cell line
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 10
//
RX   PubMed=19705962; DOI=10.1089/ten.TEA.2009.0184;
RA   May T., Butueva M., Bantner S., Markusic D., Seppen J., MacLeod R.A.F.,
RA   Weich H., Hauser H., Wirth D.;
RT   "Synthetic gene regulation circuits for control of cell expansion.";
RL   Tissue Eng. Part A 16:441-452(2010).
//
RX   PubMed=26345505; DOI=10.2144/000114326;
RA   Lieber D., Hochdorfer D., Stoehr D., Schubert A., Lotfi R., May T.,
RA   Wirth D., Sinzger C.;
RT   "A permanently growing human endothelial cell line supports productive
RT   infection with human cytomegalovirus under conditional cell growth
RT   arrest.";
RL   BioTechniques 59:127-136(2015).
//