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Cellosaurus HeLa/Fucci(CA)5 (CVCL_A7AU)

[Text version]
Cell line name HeLa/Fucci(CA)5
Synonyms HeLa/Fucci(CA)5 #16; HeLa/Fucci(CA)5#16
Accession CVCL_A7AU
Resource Identification Initiative To cite this cell line use: HeLa/Fucci(CA)5 (RRID:CVCL_A7AU)
Comments Population: African American.
Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci(CA5) (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci(CA)5 probe which consist of the fusion of AzaleaB5 to the ubiquitination domain of human CDT1 (aa 1-100) but with the Cy motif 68-RRL-70 replaced by AAA and of h2-3 to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Ser101_Leu546del and p.Arg68Ala, p.Arg69Ala and p.Leu70Ala).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; A0A6G7QPQ8; Montipora monasteriata AzaleaB5.
Transfected with: Ricordea sp. h2-3, a bright green fluorescent protein.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0058 (HeLa S3)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line

Ando R., Sakaue-Sawano A., Shoda K., Miyawaki A.
Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression.
bioRxiv 2020:03.30.015156-03.30.015156(2020)

Cell line collections (Providers) RCB; RCB4919
Encyclopedic resources Wikidata; Q107115324
Entry history
Entry creation20-May-2021
Last entry update05-Oct-2023
Version number6