ID   HeLa/Fucci(CA)5
AC   CVCL_A7AU
SY   HeLa/Fucci(CA)5 #16; HeLa/Fucci(CA)5#16
DR   RCB; RCB4919
DR   Wikidata; Q107115324
RX   PubMed=37394513;
CC   Population: African American.
CC   Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci(CA5) (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct (PubMed=37394513).
CC   Characteristics: Transfected with the Fucci(CA)5 probe which consist of the fusion of AzaleaB5 to the ubiquitination domain of human CDT1 (AA 1-100) but with the Cy motif 68-RRL-70 replaced by AAA and of h2-3 to the ubiquitination domain of human geminin (AA 1-110) (PubMed=37394513).
CC   Genetic integration: Method=Transfection; Gene=FPbase; 886AH; AzaleaB5 (Note=Red fluorescent protein from Montipora monasteriata).
CC   Genetic integration: Method=Transfection; Gene=FPbase; YQ25V; h2-3 (Note=Bright green/yellow fluorescent protein from Ricordea sp. FP2-3).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Ser101_Leu546del and p.Arg68Ala, p.Arg69Ala and p.Leu70Ala).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0058 ! HeLa S3
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 9
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RX   PubMed=37394513; DOI=10.1247/csf.23028; PMCID=PMC10958192;
RA   Ando, Ryoko
RA   Sakaue-Sawano, Asako
RA   Shoda, Keiko
RA   Miyawaki, Atsushi
RT   "Two coral fluorescent proteins of distinct colors for sharp
RT   visualization of cell-cycle progression.";
RL   Cell Struct. Funct. 48:135-144(2023).
//