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Cellosaurus HL-60(TB) (CVCL_A794)

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Cell line name HL-60(TB)
Synonyms HL-60 (TB); HL-60-TB; HL-60TB; HL-60 TB
Accession CVCL_A794
Resource Identification Initiative To cite this cell line use: HL-60(TB) (RRID:CVCL_A794)
Comments Part of: NCI-60 cancer cell line panel.
Population: Caucasian.
Doubling time: 28.6 hours (NCI-DTP=HL-60).
Omics: Genomics; Whole exome sequencing.
Omics: Metabolomics.
Omics: Phenotyping; Drug screening.
Omics: Phenotyping; Fluorescent probe library screening.
Omics: Proteomics.
Omics: Transcriptomics; lncRNA profiling; RT-qPCR platform.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (PubMed=17088437).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (PubMed=17088437).
HLA typing Source: PubMed=15748285
Class I
HLA-AA*01:01:01
HLA-BB*57:01:01
HLA-CC*06:02
Class II
HLA-DPDPB1*04:01,13:01
HLA-DQDQB1*03:03:02
Disease Adult acute myeloid leukemia with maturation (NCIt: C7961)
Acute myeloblastic leukemia with maturation (ORDO: Orphanet_98834)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0002 (HL-60)
Sex of cell Female
Age at sampling 36Y
Category Cancer cell line
STR profile Source(s): PubMed=19372543

Markers:
AmelogeninX
CSF1PO13,14
D2S133817
D3S135816
D5S81812
D7S82011,12
D8S117912,13
D13S3178,11
D16S53911
D18S5114,15
D19S43314
D21S1129,30
FGA22
TH017,8
TPOX8,11
vWA16

Run an STR similarity search on this cell line
Web pages Info; NCI DTP; NCI-60; https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
Publications

PubMed=2041050; DOI=10.1093/jnci/83.11.757
Anne Monks, Dominic A. Scudiero, Philip Skehan, Robert H. Shoemaker, Kenneth D. Paull, David T. Vistica, Curtis D. Hose, John Langley, Paul Cronise, Anne Vaigro-Wolff, Marcia Gray-Goodrich ...Show all 14 authors... , Hugh Campbell, Joseph G. Mayo, Michael R. Boyd; Show fewer authors
Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
J. Natl. Cancer Inst. 83:757-766(1991)

PubMed=9331090
Patrick M. O'Connor, Joany Jackman, Insoo Bae, Timothy Gene Myers, Sai-Jun Fan, Masato Mutoh, Dominic A. Scudiero, Anne Monks, Edward A. Sausville, John N. Weinstein, Stephen H. Friend ...Show all 13 authors... , Albert J. Fornace Jr., Kurt W. Kohn; Show fewer authors
Characterization of the p53 tumor suppressor pathway in cell lines of the National Cancer Institute anticancer drug screen and correlations with the growth-inhibitory potency of 123 anticancer agents.
Cancer Res. 57:4285-4300(1997)

PubMed=10700174; DOI=10.1038/73432
Douglas T. Ross, Uwe Scherf, Michael Bruce Eisen, Charles M. Perou, Christian Rees, Paul T. Spellman, Vishwanath R. Iyer, Stefanie S. Jeffrey, Matt van de Rijn, Mark C. Waltham, Alexander Pergamenschikov ...Show all 18 authors... , Jeffrey C.F. Lee, Deval Lashkari, Dari Shalon, Timothy Gene Myers, John N. Weinstein, David Botstein, Patrick O'Reilly Brown; Show fewer authors
Systematic variation in gene expression patterns in human cancer cell lines.
Nat. Genet. 24:227-235(2000)

PubMed=15748285; DOI=10.1186/1479-5876-3-11; PMCID=PMC555742
Sharon Adams, Fu-Meei Robbins, Deborah Chen, Devika Wagage, Susan L. Holbeck, Herbert Carpenter Morse 3rd, David Stroncek, Francesco M. Marincola;
HLA class I and II genotype of the NCI-60 cell lines.
J. Transl. Med. 3:11.1-11.8(2005)

PubMed=17088437; DOI=10.1158/1535-7163.MCT-06-0433; PMCID=PMC2705832
Ogechi N. Ikediobi, Helen R. Davies, Graham Robert Bignell, Sarah Edkins, Claire Stevens, Sarah O'Meara, Thomas Santarius, Tim Avis, Syd Barthorpe, Lisa Brackenbury, Gemma Buck ...Show all 49 authors... , Adam P. Butler, Jody Clements, Jennifer Cole, Ed Dicks, Simon Forbes, Kristian Gray, Kelly Halliday, Rachel Harrison, Katy Hills, Jonathan Hinton, Chris Hunter, Andy Jenkinson, David Jones, Vivian Kosmidou, Richard Lugg, Andrew Menzies, Tatiana Mironenko, Adrian Parker, Janet Perry, Keiran Martin Raine, David Richardson, Rebecca Shepherd, Alex Small, Raffaella Smith, Helen Solomon, Philip J. Stephens, Jon W. Teague, Calli Tofts, Jennifer Varian, Tony Webb, Sofie West, Sara Widaa, Andy Yates, William C. Reinhold, John N. Weinstein, Michael Rudolf Stratton, P. Andrew Futreal, Richard Wooster; Show fewer authors
Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.
Mol. Cancer Ther. 5:2606-2612(2006)

PubMed=19372543; DOI=10.1158/1535-7163.MCT-08-0921; PMCID=PMC4020356
Philip L. Lorenzi, William C. Reinhold, Sudhir Varma, Amy A. Hutchinson, Yves Pommier, Stephen Jacob Chanock, John N. Weinstein;
DNA fingerprinting of the NCI-60 cell line panel.
Mol. Cancer Ther. 8:713-724(2009)

PubMed=22384151; DOI=10.1371/journal.pone.0032096; PMCID=PMC3285665
Jun-Seok Lee, Yun Kyung Kim, Han Jo Kim, Siti Hajar, Yee Ling Tan, Nam-Young Kang, Shin Hui Ng, Chang No Yoon, Young-Tae Chang;
Identification of cancer cell-line origins using fluorescence image-based phenomic screening.
PLoS ONE 7:e32096.1-e32096.8(2012)

PubMed=22628656; DOI=10.1126/science.1218595; PMCID=PMC3526189
Mohit Jain, Roland Nilsson, Sonia Sharma, Nikhil Madhusudhan, Toshimori Kitami, Amanda L. Souza, Ran Kafri, Marc Wallace Kirschner, Clary B. Clish, Vamsi Krishna Mootha;
Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation.
Science 336:1040-1044(2012)

PubMed=23856246; DOI=10.1158/0008-5472.CAN-12-3342; PMCID=PMC4893961
Ogan Demir Abaan, Eric C. Polley, Sean R. Davis, Yue-Lin J. Zhu, Sven Bilke, Robert L. Walker, Marbin A. Pineda, Yevgeniy Gindin, Yuan Jiang, William C. Reinhold, Susan L. Holbeck ...Show all 15 authors... , Richard M. Simon, James H. Doroshow, Yves Pommier, Paul S. Meltzer; Show fewer authors
The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology.
Cancer Res. 73:4372-4382(2013)

PubMed=23933261; DOI=10.1016/j.celrep.2013.07.018
Amin Moghaddas Gholami, Hannes Hahne, Zhi-Xiang Wu, Florian Johann Auer, Chen Meng, Mathias Wilhelm, Bernhard Kuster;
Global proteome analysis of the NCI-60 cell line panel.
Cell Rep. 4:609-620(2013)

PubMed=24279929; DOI=10.1186/2049-3002-1-20; PMCID=PMC4178206
Sonia C. Dolfi, Leo Li-Ying Chan, Jean Qiu, Philip M. Tedeschi, Joseph Rocco Bertino, Kim Marie Hirshfield, Zoltan Nagy Oltvai, Alexei Vazquez;
The metabolic demands of cancer cells are coupled to their size and protein synthesis rates.
Cancer Metab. 1:20.1-20.13(2013)

PubMed=27377824; DOI=10.1038/sdata.2016.52; PMCID=PMC4932877
Pieter Mestdagh, Steve Lefever, Pieter-Jan Volders, Stefaan Derveaux, Jan Hellemans, Jo Vandesompele;
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.
Sci. Data 3:160052.1-160052.6(2016)

PubMed=27807467; DOI=10.1186/s13100-016-0078-4; PMCID=PMC5087121
John G. Zampella, Nemanja Rodic, Wan Rou Yang, Cheng Ran Lisa Huang, Jane Welch, Veena P. Gnanakkan, Toby Charles Cornish, Jef D. Boeke, Kathleen H. Burns;
A map of mobile DNA insertions in the NCI-60 human cancer cell panel.
Mob. DNA 7:20.1-20.11(2016)

PubMed=38861359; DOI=10.1158/0008-5472.CAN-23-3031; PMCID=PMC11292194
Mark W. Kunkel, Nathan P. Coussens, Joel Morris, Ronald C. Taylor, Thomas S. Dexheimer, Eric M. Jones, James H. Doroshow, Beverly A. Teicher;
HTS384 NCI60: the next phase of the NCI60 screen.
Cancer Res. 84:2403-2416(2024)

Cross-references
Cell line collections (Providers) NCI-DTP; HL-60
Cell line databases/resources cancercelllines; CVCL_A794
SKY/M-FISH/CGH; 2738
Anatomy/cell type resources BTO; BTO_0004119
Chemistry resources ChEMBL-Cells; CHEMBL4106363
ChEMBL-Targets; CHEMBL4296429
PubChem_Cell_line; CVCL_A794
Encyclopedic resources Wikidata; Q54889812
Gene expression databases GEO; GSM2173
GEO; GSM1178186
GEO; GSM1178187
GEO; GSM1178188
GEO; GSM1178189
GEO; GSM1178190
GEO; GSM2124666
Polymorphism and mutation databases Cosmic; 974252
Cosmic; 1175855
Cosmic; 1312323
Proteomic databases PRIDE; PXD005942
PRIDE; PXD005946
Entry history
Entry creation06-Jun-2012
Last entry update14-Aug-2025
Version number35