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Cellosaurus HEK293S GnTI- (CVCL_A785)

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Cell line name HEK293S GnTI-
Synonyms 293SGnTI(-); HEK293SG; 293SG
Accession CVCL_A785
Resource Identification Initiative To cite this cell line use: HEK293S GnTI- (RRID:CVCL_A785)
Comments Characteristics: Lacks N-acetylglucosaminyltransferase I activity and accordingly predominantly modifies glycoproteins with the Man5GlcNAc2 N-glycan. Used for the production of homogenously N-glycosylated proteins (PubMed=25182477).
Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A784 (HEK293S)
Children:
CVCL_6E38 (293SGlycoDelete)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): ATCC=CRL-3022

Markers:
AmelogeninX
CSF1PO11,12
D2S133819
D3S135815,17
D5S8188,9
D7S82011,12
D8S117912,14
D13S31712,14
D16S5399
D18S5118
D19S43318
D21S1128,30.2
FGA23
Penta D9,10
Penta E7,15
TH017,9.3
TPOX11
vWA16,19

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Publications

PubMed=12370423; DOI=10.1073/pnas.212519299; PMCID=PMC129688
Philip J. Reeves, Nico Callewaert, Roland Contreras, Har Gobind Khorana;
Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line.
Proc. Natl. Acad. Sci. U.S.A. 99:13419-13424(2002)

PubMed=25182477; DOI=10.1038/ncomms5767; PMCID=PMC4166678
Yao-Cheng Lin, Morgane Boone, Leander Meuris, Irma Lemmens, Nadine Van Roy, Arne Soete, Joke Reumers, Matthieu Moisse, Stephane Plaisance, Radoje Drmanac, Jason Chen ...Show all 16 authors... , Frank Speleman, Diether Lambrechts, Yves Van de Peer, Jan Tavernier, Nico Callewaert; Show fewer authors
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
Nat. Commun. 5:4767.1-4767.12(2014)

DOI=10.13005/bpj/1414
Jun Yuan, Wayne Wen-Yan Xu, Snake Yu-Lin Jiang, Henry Xiao-Yu Yu, H.-Fai Poon;
The scattered twelve tribes of HEK293.
Biomed. Pharmacol. J. 11:621-623(2018)

Cross-references
Cell line collections (Providers) ATCC; CRL-3022
Encyclopedic resources Wikidata; Q54882485
Experimental variables resources EFO; EFO_0022686
Entry history
Entry creation06-Jun-2012
Last entry update14-Aug-2025
Version number17