ID   HEK293S GnTI-
AC   CVCL_A785
SY   293SGnTI(-); HEK293SG; 293SG
DR   ATCC; CRL-3022
DR   Wikidata; Q54882485
RX   DOI=10.13005/bpj/1414;
RX   PubMed=12370423;
RX   PubMed=25182477;
CC   Characteristics: Lacks N-acetylglucosaminyltransferase I activity and accordingly predominantly modifies glycoproteins with the Man5GlcNAc2 N-glycan. Used for the production of homogenously N-glycosylated proteins (PubMed=25182477).
CC   Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
ST   Source(s): ATCC=CRL-3022
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 12,14
ST   D16S539: 9
ST   D18S51: 18
ST   D19S433: 18
ST   D21S11: 28,30.2
ST   D2S1338: 19
ST   D3S1358: 15,17
ST   D5S818: 8,9
ST   D7S820: 11,12
ST   D8S1179: 12,14
ST   FGA: 23
ST   Penta D: 9,10
ST   Penta E: 7,15
ST   TH01: 7,9.3
ST   TPOX: 11
ST   vWA: 16,19
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A784 ! HEK293S
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 06-06-12; Last updated: 02-05-24; Version: 14
//
RX   DOI=10.13005/bpj/1414;
RA   Yuan J., Xu W.W.-Y., Jiang S.Y.-L., Yu H.X.-Y., Poon H.-F.;
RT   "The scattered twelve tribes of HEK293.";
RL   Biomed. Pharmacol. J. 11:621-623(2018).
//
RX   PubMed=12370423; DOI=10.1073/pnas.212519299;
RA   Reeves P.J., Callewaert N., Contreras R., Khorana H.G.;
RT   "Structure and function in rhodopsin: high-level expression of
RT   rhodopsin with restricted and homogeneous N-glycosylation by a
RT   tetracycline-inducible N-acetylglucosaminyltransferase I-negative
RT   HEK293S stable mammalian cell line.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13419-13424(2002).
//
RX   PubMed=25182477; DOI=10.1038/ncomms5767;
RA   Lin Y.-C., Boone M., Meuris L., Lemmens I., Van Roy N., Soete A.,
RA   Reumers J., Moisse M., Plaisance S., Drmanac R., Chen J., Speleman F.,
RA   Lambrechts D., Van de Peer Y., Tavernier J., Callewaert N.;
RT   "Genome dynamics of the human embryonic kidney 293 lineage in response
RT   to cell biology manipulations.";
RL   Nat. Commun. 5:4767.1-4767.12(2014).
//