ID   HEK293S GnTI-
AC   CVCL_A785
SY   293SGnTI(-); HEK293SG; 293SG
DR   EFO; EFO_0022686
DR   ATCC; CRL-3022
DR   Wikidata; Q54882485
RX   DOI=10.13005/bpj/1414;
RX   PubMed=12370423;
RX   PubMed=25182477;
CC   Characteristics: Lacks N-acetylglucosaminyltransferase I activity and accordingly predominantly modifies glycoproteins with the Man5GlcNAc2 N-glycan. Used for the production of homogenously N-glycosylated proteins (PubMed=25182477).
CC   Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
CC   Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
CC   Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
ST   Source(s): ATCC=CRL-3022
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 12,14
ST   D16S539: 9
ST   D18S51: 18
ST   D19S433: 18
ST   D21S11: 28,30.2
ST   D2S1338: 19
ST   D3S1358: 15,17
ST   D5S818: 8,9
ST   D7S820: 11,12
ST   D8S1179: 12,14
ST   FGA: 23
ST   Penta D: 9,10
ST   Penta E: 7,15
ST   TH01: 7,9.3
ST   TPOX: 11
ST   vWA: 16,19
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A784 ! HEK293S
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 06-06-12; Last updated: 14-08-25; Version: 17
CH   CVCL_6E38 ! 293SGlycoDelete
//
RX   PubMed=12370423; DOI=10.1073/pnas.212519299; PMCID=PMC129688;
RA   Reeves, Philip J.
RA   Callewaert, Nico
RA   Contreras, Roland
RA   Khorana, Har Gobind
RT   "Structure and function in rhodopsin: high-level expression of
RT   rhodopsin with restricted and homogeneous N-glycosylation by a
RT   tetracycline-inducible N-acetylglucosaminyltransferase I-negative
RT   HEK293S stable mammalian cell line.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13419-13424(2002).
//
RX   PubMed=25182477; DOI=10.1038/ncomms5767; PMCID=PMC4166678;
RA   Lin, Yao-Cheng
RA   Boone, Morgane
RA   Meuris, Leander
RA   Lemmens, Irma
RA   Van Roy, Nadine
RA   Soete, Arne
RA   Reumers, Joke
RA   Moisse, Matthieu
RA   Plaisance, Stephane
RA   Drmanac, Radoje
RA   Chen, Jason
RA   Speleman, Frank
RA   Lambrechts, Diether
RA   Van de Peer, Yves
RA   Tavernier, Jan
RA   Callewaert, Nico
RT   "Genome dynamics of the human embryonic kidney 293 lineage in response
RT   to cell biology manipulations.";
RL   Nat. Commun. 5:4767.1-4767.12(2014).
//
RX   DOI=10.13005/bpj/1414;
RA   Yuan, Jun
RA   Xu, Wayne Wen-Yan
RA   Jiang, Snake Yu-Lin
RA   Yu, Henry Xiao-Yu
RA   Poon, H.-Fai
RT   "The scattered twelve tribes of HEK293.";
RL   Biomed. Pharmacol. J. 11:621-623(2018).
//