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Cellosaurus S2-DGRC-attP-25C6 (CVCL_A5UV)

[Text version]
Cell line name S2-DGRC-attP-25C6
Synonyms S2-DGRC-attP-25C6-Clone 8
Accession CVCL_A5UV
Resource Identification Initiative To cite this cell line use: S2-DGRC-attP-25C6 (RRID:CVCL_A5UV)
Comments Group: Insect cell line.
Characteristics: Using CRISPR/Cas9 a Actin5C promoter-dsRed cassette flanked by attP sites was introduced in the 25C6 locus (PubMed=33963853).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R.
Hierarchy Parent: CVCL_TZ72 (S2-DGRC)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Publications

PubMed=33963853; DOI=10.1093/g3journal/jkab161
Mariyappa D., Luhur A., Overton D., Zelhof A.C.
Generation of Drosophila attP containing cell lines using CRISPR-Cas9.
G3 (Bethesda) 11:jkab161.1-jkab161.8(2021)

Cross-references
Cell line collections (Providers) DGRC; 316
Cell line databases/resources FlyBase_Cell_line; FBtc0000316
Encyclopedic resources Wikidata; Q107116814
Entry history
Entry creation20-May-2021
Last entry update05-Oct-2023
Version number6