ID   S2-DGRC-attP-25C6
AC   CVCL_A5UV
SY   S2-DGRC-attP-25C6-Clone 8
DR   DGRC; 316
DR   FlyBase_Cell_line; FBtc0000316
DR   Wikidata; Q107116814
RX   PubMed=33963853;
CC   Group: Insect cell line.
CC   Characteristics: Using CRISPR/Cas9 a Actin5C promoter-dsRed cassette flanked by attP sites was introduced in the 25C6 locus (PubMed=33963853).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_TZ72 ! S2-DGRC
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 20-05-21; Last updated: 10-09-24; Version: 7
//
RX   PubMed=33963853; DOI=10.1093/g3journal/jkab161; PMCID=PMC8496291;
RA   Mariyappa D., Luhur A., Overton D., Zelhof A.C.;
RT   "Generation of Drosophila attP containing cell lines using
RT   CRISPR-Cas9.";
RL   G3 (Bethesda) 11:jkab161.1-jkab161.8(2021).
//