Cellosaurus ActiCre-B1 (CVCL_A4SE)
| Cell line name | ActiCre-B1 |
|---|---|
| Accession | CVCL_A4SE |
| Resource Identification Initiative | To cite this cell line use: ActiCre-B1 (RRID:CVCL_A4SE) |
| Comments | Group: Non-human primate cell line. Characteristics: Ubiquitously expresses the tamoxifen-inducible Cre-driver ERT2iCreERT2. Using CRISPR/Cas9 a IRES-ERT2iCreERT2-IRES-Hyg construct was introduced into the 3'UTR of ACTB (PubMed=33453576). Genetic integration: Method=CRISPR/Cas9; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Callithrix jacchus (White-tufted-ear marmoset) (NCBI Taxonomy: 9483) |
| Hierarchy | Parent: CVCL_B946 (CMES40) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=33453576; DOI=10.1016/j.scr.2021.102164 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107113348 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 10-Apr-2025 |
| Version number | 8 |