ID   ActiCre-B1
AC   CVCL_A4SE
DR   Wikidata; Q107113348
RX   PubMed=33453576;
CC   Group: Non-human primate cell line.
CC   Characteristics: Ubiquitously expresses the tamoxifen-inducible Cre-driver ERT2iCreERT2. Using CRISPR/Cas9 a IRES-ERT2iCreERT2-IRES-Hyg construct was introduced into the 3'UTR of ACTB (PubMed=33453576).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9483; ! Callithrix jacchus (White-tufted-ear marmoset)
HI   CVCL_B946 ! CMES40
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 8
//
RX   PubMed=33453576; DOI=10.1016/j.scr.2021.102164;
RA   Yoshimatsu S., Ohtsu K., Sato T., Yamamoto M., Sasaki E., Shiozawa S.,
RA   Okano H.;
RT   "Generation and validation of a common marmoset embryonic stem cell
RT   line ActiCre-B1 that ubiquitously expresses a tamoxifen-inducible
RT   Cre-driver.";
RL   Stem Cell Res. 51:102164-102164(2021).
//