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Cellosaurus NCCSEDi001-A-1 (CVCL_A4EG)

[Text version]
Cell line name NCCSEDi001-A-1
Synonyms NANS-2-6
Accession CVCL_A4EG
Resource Identification Initiative To cite this cell line use: NCCSEDi001-A-1 (RRID:CVCL_A4EG)
Comments From: National Chengdu Center for Safety Evaluation of Drugs; Chengdu; China.
Population: Chinese; Han.
Characteristics: Using CRISPR/Cas9 GFP-P2A-Puro was integrated in both alleles of NANS exon 1 (PubMed=33130469).
Knockout cell: Method=CRISPR/Cas9; HGNC; 19237; NANS.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A4EF (NCCSEDi001-A)
Sex of cell Male
Age at sampling 34Y
Category Induced pluripotent stem cell

PubMed=33130469; DOI=10.1016/j.scr.2020.102052
Bu Q., Zhang H.-Q., Liu Q., Dai Y.-P., Wei Q., Xue A.-Q., Huang Y., Zhong K., Huang Y.-N., Gao H., Cen X.-B.
Generation of an NANS homozygous knockout human induced pluripotent stem cell line by the insertion of GFP-P2A-Puro via CRISPR/Cas9 editing.
Stem Cell Res. 49:102052-102052(2020)

Cell line databases/resources hPSCreg; NCCSEDi001-A-1
Biological sample resources BioSamples; SAMEA7222556
Encyclopedic resources Wikidata; Q105510307
Entry history
Entry creation12-Jan-2021
Last entry update05-Oct-2023
Version number6