ID   NCCSEDi001-A-1
AC   CVCL_A4EG
SY   NANS-2-6
DR   BioSamples; SAMEA7222556
DR   hPSCreg; NCCSEDi001-A-1
DR   Wikidata; Q105510307
RX   PubMed=33130469;
CC   From: National Chengdu Center for Safety Evaluation of Drugs; Chengdu; China.
CC   Population: Chinese; Han.
CC   Characteristics: Using CRISPR/Cas9 NANS was disrupted by insertion of a GFP-P2A-PuroR construct in exon 1 of both alleles (PubMed=33130469).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:19237; NANS.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Cell type: Fibroblast; CL=CL_0000057.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A4EF ! NCCSEDi001-A
SX   Male
AG   34Y
CA   Induced pluripotent stem cell
DT   Created: 12-01-21; Last updated: 14-08-25; Version: 9
//
RX   PubMed=33130469; DOI=10.1016/j.scr.2020.102052;
RA   Bu, Qian
RA   Zhang, Hua-Qin
RA   Liu, Qian
RA   Dai, Yan-Ping
RA   Wei, Qian
RA   Xue, Ai-Qin
RA   Huang, Yan
RA   Zhong, Kai
RA   Huang, Yi-Na
RA   Gao, Hong
RA   Cen, Xiao-Bo
RT   "Generation of an NANS homozygous knockout human induced pluripotent
RT   stem cell line by the insertion of GFP-P2A-Puro via CRISPR/Cas9
RT   editing.";
RL   Stem Cell Res. 49:102052-102052(2020).
//