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Cellosaurus BT-474 Ecadherin EmGFP (CVCL_A4CL)

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Cell line name BT-474 Ecadherin EmGFP
Synonyms BT-474 ECAD-EmGFP
Accession CVCL_A4CL
Resource Identification Initiative To cite this cell line use: BT-474 Ecadherin EmGFP (RRID:CVCL_A4CL)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9 CDH1 has been tagged in one allele at the C-terminus with EmGFP. Allows to monitor the epithelial-to-mesenchymal transition (EMT) status of cells in vitro by monitoring EmGFP expression.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; GLAD6; Emerald (Note=EmGFP, green fluorescent protein derived from GFP).
Derived from site: In situ; Breast; UBERON=UBERON_0000310.
Sequence variations
  • Gene fusion; HGNC; HGNC:24618; AHCTF1 + HGNC; HGNC:736; NAAA; Name(s)=AHCTF1-NAAA (from parent cell line).
  • Gene fusion; HGNC; HGNC:16173; DOK5 + HGNC; HGNC:17579; STARD3; Name(s)=STARD3-DOK5 (from parent cell line).
  • Gene fusion; HGNC; HGNC:13178; IKZF3 + HGNC; HGNC:12649; VAPB; Name(s)=VAPB-IKZF3 (from parent cell line).
  • Gene fusion; HGNC; HGNC:16651; MRPL45 + HGNC; HGNC:16181; TRPC4AP; Name(s)=TRPC4AP-MRPL45 (from parent cell line).
  • Gene fusion; HGNC; HGNC:7609; MYO9B + HGNC; HGNC:9764; RAB22A; Name(s)=RAB22A-MYO9B (from parent cell line).
  • Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ser3094Ter (c.9281C>A); ClinVar=VCV000188440; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11621; HNF1A; Simple; p.Gln495Ter (c.1483C>T); ClinVar=VCV000586785; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6871; MAPK1; Simple; p.His61Gln (c.183C>G); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Lys111Asn (c.333G>C); ClinVar=VCV000376483; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Glu285Lys (c.853G>A); ClinVar=VCV000420133; Zygosity=Homozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0179 (BT-474)
Sex of cell Female
Age at sampling 60Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-20EMT

Markers:
AmelogeninX
CSF1PO10,11
D5S81811,13
D7S8209,12
D13S31711
D16S5399,11
TH017
TPOX8
vWA15,16

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Cross-references
Cell line collections (Providers) ATCC; HTB-20EMT
Cell line databases/resources cancercelllines; CVCL_A4CL
Encyclopedic resources Wikidata; Q105506549
Entry history
Entry creation12-Jan-2021
Last entry update10-Apr-2025
Version number9