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Cellosaurus NH32 (CVCL_A1RF)

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Cell line name NH32
Accession CVCL_A1RF
Resource Identification Initiative To cite this cell line use: NH32 (RRID:CVCL_A1RF)
Comments Population: Caucasian.
Knockout cell: Method=Promoterless gene targeting; HGNC; HGNC:11998; TP53.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06988; Escherichia coli hisD.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Transformant: ChEBI; CHEBI_132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Spleen; UBERON=UBERON_0002106.
Cell type: B-cell; CL=CL_0000236.
Sequence variations
  • Mutation; HGNC; HGNC:11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=26774668).
Disease Hereditary spherocytosis (NCIt: C97074)
Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0561 (TK6)
Sex of cell Male
Age at sampling 5Y
Category Transformed cell line
Publications

PubMed=10397247
Yao-Yu Eric Chuang, Qi Chen, Joseph P. Brown, John Michael Sedivy, Howard L. Liber;
Radiation-induced mutations at the autosomal thymidine kinase locus are not elevated in p53-null cells.
Cancer Res. 59:3073-3076(1999)

PubMed=22042714; DOI=10.1093/mutage/ger074
Kiyohiro Hashimoto, Yumi Nakajima, Rieko Uematsu, Fumio Chatani;
Difference in susceptibility to morphological changes in the nucleus to aneugens between p53-competent and p53-abrogated lymphoblastoid cell lines (TK6 and NH32 cells) in the in vitro micronucleus assay.
Mutagenesis 27:287-293(2012)

PubMed=26774668; DOI=10.1016/j.mrgentox.2015.11.006
Javier Revollo, Dayton M. Petibone, Page McKinzie, Bridgett Knox, Suzanne M. Morris, Bai-Tang Ning, Vasily N. Dobrovolsky;
Whole genome and normalized mRNA sequencing reveal genetic status of TK6, WTK1, and NH32 human B-lymphoblastoid cell lines.
Mutat. Res. Genet. Toxicol. Environ. Mutagen. 795:60-69(2016)

Cross-references
Encyclopedic resources Wikidata; Q105510331
Entry history
Entry creation12-Jan-2021
Last entry update14-Aug-2025
Version number8