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Cellosaurus WAe009-A-37 (CVCL_A1PJ)

[Text version]
Cell line name WAe009-A-37
Synonyms H9-GSX2-tTA:GFP; UMILe010-A-37
Accession CVCL_A1PJ
Resource Identification Initiative To cite this cell line use: WAe009-A-37 (RRID:CVCL_A1PJ)
Comments From: University of Milan; Milan; Italy.
Characteristics: Using CRISPR/Cas9 a tTA-turbo GFP construct was integrated in front of the stop codon of GSX2 (PubMed=33039807).
Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=33039807; DOI=10.1016/j.scr.2020.102016
Besusso D., Cossu A., Mohamed A., Cernigoj M., Codega P., Galimberti M., Campus I., Conforti P., Cattaneo E.
A CRISPR-strategy for the generation of a detectable fluorescent hESC reporter line (WAe009-A-37) for the subpallial determinant GSX2.
Stem Cell Res. 49:102016-102016(2020)

Cross-references
Cell line databases/resources hPSCreg; WAe009-A-37
Biological sample resources BioSamples; SAMEA7201185
Encyclopedic resources Wikidata; Q102115102
Entry history
Entry creation29-Oct-2020
Last entry update29-Jun-2023
Version number5