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Cellosaurus AF5 (CVCL_8831)

[Text version]
Cell line name AF5
Synonyms AF-5
Accession CVCL_8831
Resource Identification Initiative To cite this cell line use: AF5 (RRID:CVCL_8831)
Comments Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen (Note=With p.Leu156_Thr708del = T155g).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Embryonic brain, mesencephalon; UBERON=UBERON_0009616.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Sprague Dawley; RS=RS_0000681.
Sex of cell Female
Age at sampling 14FD
Category Transformed cell line
Publications

PubMed=9426306; DOI=10.1007/s004410050989
Truckenmiller M.E., Tornatore C., Wright R.D., Dillon-Carter O., Meiners S., Geller H.M., Freed W.J.
A truncated SV40 large T antigen lacking the p53 binding domain overcomes p53-induced growth arrest and immortalizes primary mesencephalic cells.
Cell Tissue Res. 291:175-189(1998)

PubMed=12061863; DOI=10.1006/exnr.2002.7898
Truckenmiller M.E., Vawter M.P., Zhang P.-S., Conejero-Goldberg C., Dillon-Carter O., Morales N., Cheadle C., Becker K.G., Freed W.J.
AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
Exp. Neurol. 175:318-337(2002)

Cross-references
Cell line collections (Providers) ABM; T0358
Anatomy/cell type resources BTO; BTO_0003422
Encyclopedic resources Wikidata; Q54608932
Entry history
Entry creation06-Jun-2012
Last entry update10-Apr-2025
Version number13