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Cellosaurus AF5 (CVCL_8831)

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Cell line name AF5
Synonyms AF-5
Accession CVCL_8831
Resource Identification Initiative To cite this cell line use: AF5 (RRID:CVCL_8831)
Comments Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen (Note=With p.Leu156_Thr708del = T155g).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Embryonic brain, mesencephalon; UBERON=UBERON_0009616.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Sprague Dawley; RS=RS_0000681.
Sex of cell Female
Age at sampling 14FD
Category Transformed cell line
Publications

PubMed=9426306; DOI=10.1007/s004410050989
Mary Ellen Truckenmiller, Carlo Tornatore, Renee Darnell Wright, Ora Dillon-Carter, Sally Meiners, Herbert M. Geller, William J. Freed;
A truncated SV40 large T antigen lacking the p53 binding domain overcomes p53-induced growth arrest and immortalizes primary mesencephalic cells.
Cell Tissue Res. 291:175-189(1998)

PubMed=12061863; DOI=10.1006/exnr.2002.7898
Mary Ellen Truckenmiller, Marquis Philip Vawter, Pei-Su Zhang, Concha Conejero-Goldberg, Ora Dillon-Carter, Nelly Morales, Christopher Cheadle, Kevin G. Becker, William J. Freed;
AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
Exp. Neurol. 175:318-337(2002)

PubMed=22863662; DOI=10.1111/j.1582-4934.2012.01607.x; PMCID=PMC4118228
Asir Alwin Prem Anand, S. Gowri Sankar, Kokila V. Vani;
Immortalization of neuronal progenitors using SV40 large T antigen and differentiation towards dopaminergic neurons.
J. Cell. Mol. Med. 16:2592-2610(2012)

Cross-references
Cell line collections (Providers) ABM; T0358
Anatomy/cell type resources BTO; BTO_0003422
Encyclopedic resources Wikidata; Q54608932
Entry history
Entry creation06-Jun-2012
Last entry update14-Aug-2025
Version number14