ID   AF5
AC   CVCL_8831
SY   AF-5
DR   BTO; BTO_0003422
DR   ABM; T0358
DR   Wikidata; Q54608932
RX   PubMed=9426306;
RX   PubMed=12061863;
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen (Note=With p.Leu156_Thr708del = T155g).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Embryonic brain, mesencephalon; UBERON=UBERON_0009616.
CC   Breed/subspecies: Sprague Dawley; RS=RS_0000681.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
SX   Female
AG   14FD
CA   Transformed cell line
DT   Created: 06-06-12; Last updated: 10-04-25; Version: 13
//
RX   PubMed=9426306; DOI=10.1007/s004410050989;
RA   Truckenmiller M.E., Tornatore C., Wright R.D., Dillon-Carter O.,
RA   Meiners S., Geller H.M., Freed W.J.;
RT   "A truncated SV40 large T antigen lacking the p53 binding domain
RT   overcomes p53-induced growth arrest and immortalizes primary
RT   mesencephalic cells.";
RL   Cell Tissue Res. 291:175-189(1998).
//
RX   PubMed=12061863; DOI=10.1006/exnr.2002.7898;
RA   Truckenmiller M.E., Vawter M.P., Zhang P.-S., Conejero-Goldberg C.,
RA   Dillon-Carter O., Morales N., Cheadle C., Becker K.G., Freed W.J.;
RT   "AF5, a CNS cell line immortalized with an N-terminal fragment of SV40
RT   large T: growth, differentiation, genetic stability, and gene
RT   expression.";
RL   Exp. Neurol. 175:318-337(2002).
//