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Cellosaurus NCOL-1 (CVCL_8375)

[Text version]
Cell line name NCOL-1
Synonyms Normal COLon-1
Accession CVCL_8375
Resource Identification Initiative To cite this cell line use: NCOL-1 (RRID:CVCL_8375)
Comments Problematic cell line: Contaminated. Shown to be a LoVo derivative (PubMed=15771911; PubMed=20143388). Originally thought to originate from a normal colonic mucosa sample.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00243.
Population: Caucasian.
Derived from site: Metastatic; Left supraclavicular lymph node; UBERON=UBERON_8480056.
Sequence variations
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Arg1114Ter (c.3340C>T); ClinVar=VCV000236589; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Met1431fs*42 (c.4289delC) (p.T1430fs); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Arg2816Gln (c.8447G>A); ClinVar=VCV000419681; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 914; B2M; Simple; p.Leu15Phefs*41 (c.43_44delCT); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6768; SMAD2; Simple; p.Ala292Val (c.875C>T); Zygosity=Heterozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0399 (LoVo)
Sex of cell Male
Age at sampling 56Y
Category Cancer cell line
STR profile Source(s): PubMed=15771911

D8S11799,10 (PubMed=15771911_clone_b)
10 (PubMed=15771911_clone_a)
D19S43313,15 (PubMed=15771911_clone_b)
14,15 (PubMed=15771911_clone_a)
D21S1128,29,30.2,31.2 (PubMed=15771911_clone_b)
29,31.2 (PubMed=15771911_clone_a)
FGA17,20 (PubMed=15771911_clone_a)
18,20 (PubMed=15771911_clone_b)
SE3318.2,19.2,20.2,25.2 (PubMed=15771911_clone_b)
19.2,24.2 (PubMed=15771911_clone_a)

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx

PubMed=8812628; DOI=10.1006/jsre.1996.0323
Deveney C.W., Rand-Luby L., Rutten M.J., Luttropp C.A., Fowler W.M., Land J., Meichsner C.L., Farahmand M., Sheppard B.C., Crass R.A., Deveney K.E.
Establishment of human colonic epithelial cells in long-term culture.
J. Surg. Res. 64:161-169(1996)

PubMed=15771911; DOI=10.1016/j.cancergencyto.2004.08.023
Melcher R., Maisch S., Koehler S., Bauer M., Steinlein C., Schmid M., Kudlich T., Schauber J., Luehrs H., Menzel T., Scheppach W.
SKY and genetic fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1.
Cancer Genet. Cytogenet. 158:84-87(2005)

PubMed=16271966; DOI=10.1016/j.cancergencyto.2005.06.003
Wenzel U., Daniel H.
Reconsidering cell line cross-contamination in NCOL-1.
Cancer Genet. Cytogenet. 163:95-96(2005)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cell line databases/resources cancercelllines; CVCL_8375
Cell_Model_Passport; SIDM00838
Biological sample resources BioSample; SAMN03151825
Encyclopedic resources Wikidata; Q54908245
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number28