| Cell line name |
NCOL-1 |
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| Synonyms |
Normal COLon-1 |
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| Accession |
CVCL_8375 |
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| Resource Identification Initiative |
To cite this cell line use: NCOL-1 (RRID:CVCL_8375) |
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| Comments |
Problematic cell line: Contaminated. Shown to be a LoVo derivative (PubMed=15771911; PubMed=20143388). Originally thought to originate from a normal colonic mucosa sample.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00243.
Population: Caucasian.
Derived from site: Metastatic; Left supraclavicular lymph node; UBERON=UBERON_8480056. |
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| Sequence variations |
- Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:583; APC; Simple; p.Arg1114Ter (c.3340C>T); ClinVar=VCV000236589; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:583; APC; Simple; p.Met1431fs*42 (c.4289delC) (p.T1430fs); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:583; APC; Simple; p.Arg2816Gln (c.8447G>A); ClinVar=VCV000419681; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:914; B2M; Simple; p.Leu15Phefs*41 (c.43_44delCT); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:6768; SMAD2; Simple; p.Ala292Val (c.875C>T); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
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| Disease |
Colon adenocarcinoma (NCIt: C4349) |
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| Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
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| Hierarchy |
Parent: CVCL_0399 (LoVo) |
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| Sex of cell |
Male |
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| Age at sampling |
56Y |
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| Category |
Cancer cell line |
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| STR profile |
Source(s): PubMed=15771911
Markers:| Amelogenin | X,Y |
| D2S1338 | 17,18 |
| D3S1358 | 14,17 |
| D8S1179 | 9,10 (PubMed=15771911_clone_b)
10 (PubMed=15771911_clone_a) |
| D16S539 | 9,12 |
| D18S51 | 13,18 |
| D19S433 | 13,15 (PubMed=15771911_clone_b)
14,15 (PubMed=15771911_clone_a) |
| D21S11 | 28,29,30.2,31.2 (PubMed=15771911_clone_b)
29,31.2 (PubMed=15771911_clone_a) |
| FGA | 17,20 (PubMed=15771911_clone_a)
18,20 (PubMed=15771911_clone_b) |
| SE33 | 18.2,19.2,20.2,25.2 (PubMed=15771911_clone_b)
19.2,24.2 (PubMed=15771911_clone_a) |
| TH01 | 9.3 |
| vWA | 17,18 |
Run an STR similarity search on this cell line |
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| Web pages |
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx |
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| Publications | PubMed=8812628; DOI=10.1006/jsre.1996.0323
Deveney C.W., Rand-Luby L., Rutten M.J., Luttropp C.A., Fowler W.M., Land J., Meichsner Meller C.L., Farahmand M., Sheppard B.C., Crass R.A., Deveney K.E.
Establishment of human colonic epithelial cells in long-term culture.
J. Surg. Res. 64:161-169(1996) PubMed=15771911; DOI=10.1016/j.cancergencyto.2004.08.023
Melcher R., Maisch S., Koehler S., Bauer M., Steinlein C., Schmid M., Kudlich T., Schauber J., Luehrs H., Menzel T., Scheppach W.
SKY and genetic fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1.
Cancer Genet. Cytogenet. 158:84-87(2005) PubMed=16271966; DOI=10.1016/j.cancergencyto.2005.06.003
Wenzel U., Daniel H.
Reconsidering cell line cross-contamination in NCOL-1.
Cancer Genet. Cytogenet. 163:95-96(2005) PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010) |
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| Cross-references |
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| Cell line databases/resources |
cancercelllines; CVCL_8375
Cell_Model_Passport; SIDM00838
|
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| Biological sample resources |
BioSample; SAMN03151825
|
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| Encyclopedic resources |
Wikidata; Q54908245
|
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| Entry history |
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| Entry creation | 04-Apr-2012 |
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| Last entry update | 10-Apr-2025 |
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| Version number | 31 |
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