ID   NCOL-1
AC   CVCL_8375
SY   Normal COLon-1
DR   BioSample; SAMN03151825
DR   cancercelllines; CVCL_8375
DR   Cell_Model_Passport; SIDM00838
DR   Wikidata; Q54908245
RX   PubMed=8812628;
RX   PubMed=15771911;
RX   PubMed=16271966;
RX   PubMed=20143388;
WW   Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
CC   Problematic cell line: Contaminated. Shown to be a LoVo derivative (PubMed=15771911; PubMed=20143388). Originally thought to originate from a normal colonic mucosa sample.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00243.
CC   Population: Caucasian.
CC   Sequence variation: Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Arg1114Ter (c.3340C>T); ClinVar=VCV000236589; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Met1431fs*42 (c.4289delC) (p.T1430fs); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Arg2816Gln (c.8447G>A); ClinVar=VCV000419681; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:914; B2M; Simple; p.Leu15Phefs*41 (c.43_44delCT); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6768; SMAD2; Simple; p.Ala292Val (c.875C>T); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
CC   Derived from site: Metastatic; Left supraclavicular lymph node; UBERON=UBERON_8480056.
ST   Source(s): PubMed=15771911
ST   Amelogenin: X,Y
ST   D16S539: 9,12
ST   D18S51: 13,18
ST   D19S433: 13,15 (PubMed=15771911_clone_b)
ST   D19S433: 14,15 (PubMed=15771911_clone_a)
ST   D21S11: 28,29,30.2,31.2 (PubMed=15771911_clone_b)
ST   D21S11: 29,31.2 (PubMed=15771911_clone_a)
ST   D2S1338: 17,18
ST   D3S1358: 14,17
ST   D8S1179: 9,10 (PubMed=15771911_clone_b)
ST   D8S1179: 10 (PubMed=15771911_clone_a)
ST   FGA: 17,20 (PubMed=15771911_clone_a)
ST   FGA: 18,20 (PubMed=15771911_clone_b)
ST   SE33: 18.2,19.2,20.2,25.2 (PubMed=15771911_clone_b)
ST   SE33: 19.2,24.2 (PubMed=15771911_clone_a)
ST   TH01: 9.3
ST   vWA: 17,18
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0399 ! LoVo
SX   Male
AG   56Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 31
//
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis, Amanda
RA   Theodosopoulos, George
RA   Atkin, Isobel
RA   Drexler, Hans Gunther
RA   Kohara, Arihiro
RA   MacLeod, Roderick A.F.
RA   Masters, John R.W.
RA   Nakamura, Yukio
RA   Reid, Yvonne A.
RA   Reddel, Roger Robert
RA   Freshney, Robert Ian
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
//
RX   PubMed=15771911; DOI=10.1016/j.cancergencyto.2004.08.023;
RA   Melcher, Ralph
RA   Maisch, Stefanie
RA   Koehler, Sigrid
RA   Bauer, Martin
RA   Steinlein, Claus
RA   Schmid, Michael
RA   Kudlich, Theodor
RA   Schauber, Jurgen
RA   Luehrs, Hardi
RA   Menzel, Thomas
RA   Scheppach, Wolfgang
RT   "SKY and genetic fingerprinting reveal a cross-contamination of the
RT   putative normal colon epithelial cell line NCOL-1.";
RL   Cancer Genet. Cytogenet. 158:84-87(2005).
//
RX   PubMed=16271966; DOI=10.1016/j.cancergencyto.2005.06.003;
RA   Wenzel, Uwe
RA   Daniel, Hannelore
RT   "Reconsidering cell line cross-contamination in NCOL-1.";
RL   Cancer Genet. Cytogenet. 163:95-96(2005).
//
RX   PubMed=8812628; DOI=10.1006/jsre.1996.0323;
RA   Deveney, Clifford W.
RA   Rand-Luby, Leslie
RA   Rutten, Michael J.
RA   Luttropp, Cheryl A.
RA   Fowler, Wendy M.
RA   Land, John
RA   Meichsner Meller, Camie L.
RA   Farahmand, Mehrdad
RA   Sheppard, Brett C.
RA   Crass, Richard A.
RA   Deveney, Karen E.
RT   "Establishment of human colonic epithelial cells in long-term
RT   culture.";
RL   J. Surg. Res. 64:161-169(1996).
//