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Cellosaurus CHP-100 (CVCL_7166)

[Text version]
Cell line name CHP-100
Synonyms CHP 100; CHP100; Children's Hospital of Philadelphia-100; NB-100
Accession CVCL_7166
Resource Identification Initiative To cite this cell line use: CHP-100 (RRID:CVCL_7166)
Comments Problematic cell line: Misclassified. Originally thought to originate from a neuroblastoma but shown to be from an Ewing sarcoma (PubMed=33460449). Was also partially contaminated as some stocks where shown to be IMR-32 derivatives.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00382.
Doubling time: 45.8 hours (PubMed=10079); ~36 hours (DSMZ=ACC-830).
Omics: Array-based CGH.
Derived from site: In situ; Bone, spine, epidural space; UBERON=UBERON_0003691.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Hemizygous (PubMed=19787792).
  • Gene fusion; HGNC; 3508; EWSR1 + HGNC; 3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 2 fusion (Ex7/Ex5) (PubMed=26428435; PubMed=33460449).
  • Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=19787792).
Disease Ewing sarcoma (NCIt: C4817)
Ewing sarcoma (ORDO: Orphanet_319)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_IV94 (CHP-100L)CVCL_IV95 (CHP-100S)
Sex of cell Female
Age at sampling 12Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-830; PubMed=19787792; PubMed=33460449

Penta D11,14
Penta E11,12
vWA17,19 (PubMed=19787792; PubMed=33460449)
17,18,19 (DSMZ=ACC-830)

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx

Schlesinger H.R., Gerson J.M., Moorhead P.S., Maguire H., Hummeler K.
Establishment and characterization of human neuroblastoma cell lines.
Cancer Res. 36:3094-3100(1976)

Biedler J.L., Meyers M.B., Spengler B.A.
Homogeneously staining regions and double minute chromosomes, prevalent cytogenetic abnormalities of human neuroblastoma cells.
(In) Advances in cellular neurobiology, Vol. 4; Fedoroff S., Hertz L. (eds.); pp.267-307; Academic Press; New York (1983)

PubMed=2777647; DOI=10.1016/0360-3016(89)90111-9
Schwachofer J.H.M., Crooijmans R.P.M.A., van Gasteren J.J.M., Hoogenhout J., Jerusalem C.R., Kal H.B., Theeuwes A.G.M.
Repair of sublethal damage in two human tumor cell lines grown as multicellular spheroids.
Int. J. Radiat. Oncol. Biol. Phys. 17:591-595(1989)

Schwachofer J.H.M., Crooijmans R.P.M.A., Hoogenhout H., Kal H.B., Schaapveld R.Q.J., Wessels J.
Differences in repair of radiation induced damage in two human tumor cell lines as measured by cell survival and alkaline DNA unwinding.
Strahlenther. Onkol. 167:35-40(1991)

Israel M.A., Thiele C.J.
Tumor cell lines of the peripheral nervous system.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.43-78; Academic Press; New York (1994)

PubMed=19787792; DOI=10.1002/gcc.20717
Ottaviano L., Schaefer K.-L., Gajewski M., Huckenbeck W., Baldus S.E., Rogel U., Mackintosh C., de Alava E., Myklebost O., Kresse S.H., Meza-Zepeda L.A., Serra M., Cleton-Jansen A.-M., Hogendoorn P.C.W., Buerger H., Aigner T., Gabbert H.E., Poremba C.
Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.
Genes Chromosomes Cancer 49:40-51(2010)

PubMed=21822310; DOI=10.1038/onc.2011.317
Mackintosh C., Ordonez J.L., Garcia-Dominguez D.J., Sevillano V., Llombart-Bosch A., Szuhai K., Scotlandi K., Alberghini M., Sciot R., Sinnaeve F., Hogendoorn P.C.W., Picci P., Knuutila S., Dirksen U., Debiec-Rychter M., Schaefer K.-L., de Alava E.
1q gain and CDT2 overexpression underlie an aggressive and highly proliferative form of Ewing sarcoma.
Oncogene 31:1287-1298(2012)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074
Teicher B.A., Polley E.C., Kunkel M., Evans D., Silvers T.E., Delosh R.M., Laudeman J., Ogle C., Reinhart R., Selby M., Connelly J., Harris E., Monks A., Morris J.
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=26428435; DOI=10.1016/j.ejca.2015.08.020
Sand L.G.L., Scotlandi K., Berghuis D., Snaar-Jagalska B.E., Picci P., Schmidt T., Szuhai K., Hogendoorn P.C.W.
CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients.
Eur. J. Cancer 51:2624-2633(2015)

PubMed=33460449; DOI=10.1002/ijc.33474
Dirks W.G., Capes-Davis A., Eberth S., Fahnrich S., Wilting J., Nagel S., Steenpass L., Becker J.
Cross-contamination meets misclassification: awakening of CHP-100 from sleeping beauty sleep -- a reviewed model for Ewing's sarcoma.
Int. J. Cancer 148:2608-2613(2021)

Cell line collections (Providers) DSMZ; ACC-830
ECACC; 06122001 - Discontinued
Cell line databases/resources CLO; CLO_0007944
CLDB; cl3642
cancercelllines; CVCL_7166
DSMZCellDive; ACC-830
Anatomy/cell type resources BTO; BTO:0005253
Biological sample resources BioSample; SAMN03151800
Encyclopedic resources Wikidata; Q54813260
Gene expression databases GEO; GSM510003
GEO; GSM1676329
GEO; GSM1701661
Polymorphism and mutation databases Cosmic; 1167419
IARC_TP53; 27037
Progenetix; CVCL_7166
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number28