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Cellosaurus CHP-100 (CVCL_7166)

[Text version]
Cell line name CHP-100
Synonyms CHP 100; CHP100; Children's Hospital of Philadelphia-100; NB-100
Accession CVCL_7166
Resource Identification Initiative To cite this cell line use: CHP-100 (RRID:CVCL_7166)
Comments Problematic cell line: Misclassified. Originally thought to originate from a neuroblastoma but shown to be from an Ewing sarcoma (PubMed=33460449). Was also partially contaminated as some stocks where shown to be IMR-32 derivatives.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00382.
Doubling time: 45.8 hours (PubMed=10079); ~36 hours (DSMZ=ACC-830).
Omics: Variations; Array-based CGH.
Derived from site: In situ; Bone, spine, epidural space; UBERON=UBERON_0003691.
Sequence variations
Disease Ewing sarcoma (NCIt: C4817)
Ewing sarcoma (ORDO: Orphanet_319)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_IV94 (CHP-100L)CVCL_IV95 (CHP-100S)
Sex of cell Female
Age at sampling 12Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-830; PubMed=19787792; PubMed=33460449

Markers:
AmelogeninX
CSF1PO12,13
D2S133818,21
D3S135816
D5S81811,12
D7S8209,10
D8S117912,15
D13S31713
D16S53910
D18S5112,17
D19S43313
D21S1129
FGA22,25
Penta D11,14
Penta E11,12
SE3317,19
TH016,9.3
TPOX8,9
vWA17,18,19 (DSMZ=ACC-830)
17,19 (PubMed=19787792; PubMed=33460449)

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=10079
Harvey R. Schlesinger, James M. Gerson, Paul Sidney Moorhead, Henry Maguire, Klaus Hummeler;
Establishment and characterization of human neuroblastoma cell lines.
Cancer Res. 36:3094-3100(1976)

DOI=10.1016/B978-0-12-008304-6.50015-4
June Lee Biedler, Marian B. Meyers, Barbara A. Spengler;
Homogeneously staining regions and double minute chromosomes, prevalent cytogenetic abnormalities of human neuroblastoma cells.
(In book chapter) Advances in cellular neurobiology, Vol. 4; Fedoroff, Sergey & Hertz, Leif (eds.); pp.267-307; Academic Press; New York; USA (1983)

PubMed=2777647; DOI=10.1016/0360-3016(89)90111-9
Johannes Henricus Maria Schwachofer, Richard P.M.A. Crooijmans, J.J.M. van Gasteren, Jacob Hoogenhout, Christoph R. Jerusalem, Henk B. Kal, Ad G.M. Theeuwes;
Repair of sublethal damage in two human tumor cell lines grown as multicellular spheroids.
Int. J. Radiat. Oncol. Biol. Phys. 17:591-595(1989)

PubMed=1992541
Johannes Henricus Maria Schwachofer, Richard P.M.A. Crooijmans, Jacob Hoogenhout, Henk B. Kal, Roel Q.J. Schaapveld, Johannes M.C. Wessels;
Differences in repair of radiation induced damage in two human tumor cell lines as measured by cell survival and alkaline DNA unwinding.
Strahlenther. Onkol. 167:35-40(1991)

DOI=10.1016/B978-0-12-333530-2.50006-X
Mark A. Israel, Carol Jeanne Thiele;
Tumor cell lines of the peripheral nervous system.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.43-78; Academic Press; New York; USA (1994)

PubMed=19787792; DOI=10.1002/gcc.20717
Laura Ottaviano, Karl-Ludwig Schaefer, Melanie Gajewski, Wolfgang Huckenbeck, Stefan E. Baldus, Uwe Rogel, Carlos Mackintosh, Enrique de Alava, Ola Myklebost, Stine Henrichson Kresse, Leonardo Andres Meza-Zepeda ...Show all 18 authors... , Massimo Serra, Anne-Marie Cleton-Jansen, Pancras Cornelis Wilhelmus Hogendoorn, Horst Buerger, Thomas Aigner, Helmut Erich Gabbert, Christopher Poremba; Show fewer authors
Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.
Genes Chromosomes Cancer 49:40-51(2010)

PubMed=21822310; DOI=10.1038/onc.2011.317
Carlos Mackintosh, Jose L. Ordonez, Daniel J. Garcia-Dominguez, Vicky Sevillano, Antonio Llombart-Bosch, Karoly Szuhai, Katia Scotlandi, Marco Alberghini, Raf Sciot, Friedl Sinnaeve, Pancras Cornelis Wilhelmus Hogendoorn ...Show all 17 authors... , Piero Picci, Sakari Knuutila, Uta Dirksen, Maria Debiec-Rychter, Karl-Ludwig Schaefer, Enrique de Alava; Show fewer authors
1q gain and CDT2 overexpression underlie an aggressive and highly proliferative form of Ewing sarcoma.
Oncogene 31:1287-1298(2012)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074; PMCID=PMC4636476
Beverly A. Teicher, Eric C. Polley, Mark W. Kunkel, David Evans, Thomas E. Silvers, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Michael Selby, John Connelly ...Show all 14 authors... , Erik Harris, Anne Monks, Joel Morris; Show fewer authors
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=26428435; DOI=10.1016/j.ejca.2015.08.020
Laurens G.L. Sand, Katia Scotlandi, Dagmar Berghuis, B. Ewa Snaar-Jagalska, Piero Picci, Thomas Schmidt, Karoly Szuhai, Pancras Cornelis Wilhelmus Hogendoorn;
CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients.
Eur. J. Cancer 51:2624-2633(2015)

PubMed=33460449; DOI=10.1002/ijc.33474
Wilhelm Gerhard Dirks, Amanda Capes-Davis, Sonja Eberth, Silke Fahnrich, Jorg Wilting, Stefan Nagel, Laura Steenpass, Jennifer Becker;
Cross-contamination meets misclassification: awakening of CHP-100 from sleeping beauty sleep -- a reviewed model for Ewing's sarcoma.
Int. J. Cancer 148:2608-2613(2021)

Cross-references
Cell line collections (Providers) DSMZ; ACC-830
ECACC; 06122001 - Discontinued
Cell line databases/resources CLO; CLO_0007944
CLDB; cl3642
cancercelllines; CVCL_7166
DSMZCellDive; ACC-830
Anatomy/cell type resources BTO; BTO_0005253
Biological sample resources BioSample; SAMN03151800
Encyclopedic resources Wikidata; Q54813260
Gene expression databases GEO; GSM510003
GEO; GSM1676329
GEO; GSM1701661
Polymorphism and mutation databases Cosmic; 1167419
IARC_TP53; 27037
Progenetix; CVCL_7166
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number30