Cellosaurus cell line HTR-8/SVneo (CVCL

Cellosaurus HTR-8/SVneo (CVCL_7162)

[Text version]

Cell line name

HTR-8/SVneo

Synonyms

HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn

Accession

CVCL_7162

Resource Identification Initiative

To cite this cell line use: HTR-8/SVneo (RRID:CVCL_7162)

Comments

Part of: ENCODE project common cell types; tier 3.
Characteristics: Useful to study trophoblast and placental biology (ATCC=CRL-3271).
Characteristics: Contains both epithelial and mesenchymal-like cell populations (PubMed=28161053).
Karyotypic information: Near triploid karyotype (PubMed=33096371).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
Omics: Genomics; DNA methylation analysis.
Derived from site: In situ; Trophoblast; UBERON=UBERON_0000088.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_D728 (HTR-8)
Children:

CVCL_F0D6 (HTR8/SVneo/EGFP)	CVCL_F0D7 (HTR8/SVneo/HRASV12)	CVCL_E1LE (HyCyte HTR-8/SVneo KO-hCGAS)
CVCL_D9WU (Ubigene HTR-8/SVneo EPO KO)	CVCL_D9WV (Ubigene HTR-8/SVneo GSDMB KO)	CVCL_D9WW (Ubigene HTR-8/SVneo GSDMD KO)
CVCL_D9WX (Ubigene HTR-8/SVneo SMAD4 KO)	CVCL_D9WY (Ubigene HTR-8/SVneo TREM1 KO)

Sex of cell

Sex unspecified

Age at sampling

6-12FW

Category

Transformed cell line

STR profile

Source(s): ATCC=CRL-3271; PubMed=33810901

Markers:

Amelogenin	X
CSF1PO	12
D1S1656	16,17.3
D2S441	11
D2S1338	23,24
D3S1358	16
D5S818	12
D7S820	12
D8S1179	13,15
D10S1248	16
D12S391	19
D13S317	9,12
D16S539	13
D18S51	13
D19S433	14,15
D21S11	29,30
D22S1045	15
FGA	21,23
Penta D	9,12
Penta E	7,13
SE33	25.2,27.2
TH01	6,9.3
TPOX	8
vWA	13,18 (ATCC=CRL-3271) 18 (PubMed=33810901)

Web pages

Info; -; -; https://articles.sonidel.com/electroporation/nepa21-electroporator/cell-lines-htr-8-svneo-human-trophoblast-cells/
Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/HTR8svn_Crawford_protocol.pdf

Publications

PubMed=7684692; DOI=10.1006/excr.1993.1139
Charles H. Graham, Teresa S. Hawley, Robert G. Hawley, John R. MacDougall, Robert S. Kerbel, Nelson Khoo, Peeyush K. Lala;
Establishment and characterization of first trimester human trophoblast cells with extended lifespan.
Exp. Cell Res. 206:204-211(1993)

PubMed=23325432; DOI=10.1101/gr.147942.112; PMCID=PMC3589544
Katherine E. Varley, Jason Gertz, Kevin M. Bowling, Stephanie L. Parker, Timothy E. Reddy, Florencia Pauli-Behn, Marie K. Cross, Brian A. Williams, John A. Stamatoyannopoulos, Gregory E. Crawford, Devin Michael Absher ...Show all 13 authors... , Barbara Jane Wold, Richard M. Myers; Show fewer authors
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=28161053; DOI=10.1016/j.placenta.2016.12.007
Wassim Abou-Kheir, Joanna Barrak, Ola Hadadeh, Georges Daoud;
HTR-8/SVneo cell line contains a mixed population of cells.
Placenta 50:1-7(2017)

PubMed=33096371; DOI=10.1016/j.placenta.2020.10.011
Maja Weber, Anja Weise, Faezeh Vasheghani, Claudia Gohner, Justine Sayuri Fitzgerald, Thomas Liehr, Udo R. Markert;
Cytogenomics of six human trophoblastic cell lines.
Placenta 103:72-75(2021)

PubMed=33810901; DOI=10.1016/j.placenta.2021.02.022
Jana Pastuschek, Olivia Nonn, Ruby N. Gutierrez-Samudio, Jose Martin Murrieta-Coxca, Jasmin Muller, Juliane Sanft, Berthold Huppertz, Udo R. Markert, Tanja Groten, Diana M. Morales-Prieto;
Molecular characteristics of established trophoblast-derived cell lines.
Placenta 108:122-133(2021)

Cross-references

Cell line collections (Providers)

ATCC; CRL-3271
CLS; 305221
Ubigene; YC-C050

Cell line databases/resources

TOKU-E; 4099

Anatomy/cell type resources

BTO; BTO_0004125

Biological sample resources

ENCODE; ENCBS331AAA

Encyclopedic resources

Wikidata; Q54896677

Experimental variables resources