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Cellosaurus NE-MD (CVCL_6C06)

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Cell line name NE-MD
Synonyms nNOS-EGFP Expressing Macula Densa
Accession CVCL_6C06
Resource Identification Initiative To cite this cell line use: NE-MD (RRID:CVCL_6C06)
Comments Characteristics: Expresses GFP under the control of the Nos1 promoter (PubMed=16412259).
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Derived from site: In situ; Kidney, macula densa; UBERON=UBERON_0002335.
Cell type: Epithelial cell of distal tubule; CL=CL_0002305.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6 SV40-tsA58 transgenic.
Category Conditionally immortalized cell line
Publications

PubMed=16412259; DOI=10.2170/jjphysiol.R2138
Yukiko Yasuoka, Hideaki Kawada, Yoshiro Suzuki, Masahiro Sato, Hitoshi Endou, Masuo Obinata, Katsumasa Kawahara;
Establishment of a mouse macula densa cell line with an nNOS promoter driving EGFP expression.
Jpn. J. Physiol. 55:365-372(2005)

PubMed=17233813; DOI=10.1111/j.1349-7006.2007.00399.x; PMCID=PMC11159456
Masuo Obinata;
The immortalized cell lines with differentiation potentials: their establishment and possible application.
Cancer Sci. 98:275-283(2007)

PubMed=19340543; DOI=10.1007/s12576-009-0022-1; PMCID=PMC10716943
Hideaki Kawada, Yukiko Yasuoka, Hidekazu Fukuda, Katsumasa Kawahara;
Low [NaCl]-induced neuronal nitric oxide synthase (nNOS) expression and NO generation are regulated by intracellular pH in a mouse macula densa cell line (NE-MD).
J. Physiol. Sci. 59:165-173(2009)

Cross-references
Encyclopedic resources Wikidata; Q54930368
Entry history
Entry creation23-Feb-2016
Last entry update14-Aug-2025
Version number11