ID   NE-MD
AC   CVCL_6C06
SY   nNOS-EGFP Expressing Macula Densa
DR   Wikidata; Q54930368
RX   PubMed=16412259;
RX   PubMed=17233813;
RX   PubMed=19340543;
CC   Characteristics: Expresses GFP under the control of the Nos1 promoter (PubMed=16412259).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
CC   Derived from site: In situ; Kidney, macula densa; UBERON=UBERON_0002335.
CC   Cell type: Epithelial cell of distal tubule; CL=CL_0002305.
CC   Breed/subspecies: C57BL/6 SV40-tsA58 transgenic.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
CA   Conditionally immortalized cell line
DT   Created: 23-02-16; Last updated: 10-09-24; Version: 10
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RX   PubMed=16412259; DOI=10.2170/jjphysiol.R2138;
RA   Yasuoka Y., Kawada H., Suzuki Y., Sato M., Endou H., Obinata M.,
RA   Kawahara K.;
RT   "Establishment of a mouse macula densa cell line with an nNOS promoter
RT   driving EGFP expression.";
RL   Jpn. J. Physiol. 55:365-372(2005).
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RX   PubMed=17233813; DOI=10.1111/j.1349-7006.2007.00399.x; PMCID=PMC11159456;
RA   Obinata M.;
RT   "The immortalized cell lines with differentiation potentials: their
RT   establishment and possible application.";
RL   Cancer Sci. 98:275-283(2007).
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RX   PubMed=19340543; DOI=10.1007/s12576-009-0022-1; PMCID=PMC10716943;
RA   Kawada H., Yasuoka Y., Fukuda H., Kawahara K.;
RT   "Low [NaCl]-induced neuronal nitric oxide synthase (nNOS) expression
RT   and NO generation are regulated by intracellular pH in a mouse macula
RT   densa cell line (NE-MD).";
RL   J. Physiol. Sci. 59:165-173(2009).
//