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Cellosaurus OCM-8 (CVCL_6938)

[Text version]
Cell line name OCM-8
Synonyms OCM8; Ocm8; ocm-8
Accession CVCL_6938
Resource Identification Initiative To cite this cell line use: OCM-8 (RRID:CVCL_6938)
Comments Problematic cell line: Contaminated. Shown to be a SK-MEL-28 derivative (PubMed=22236444 and STR profile from PubMed=18689700). Originally thought to originate from an uveal melanoma.
From: Kan-Mitchell, June; Wayne State University; Detroit; USA.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00504.
Population: Caucasian.
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Cell type: Melanocyte of skin; CL=CL_1000458.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (PubMed=22236444).
  • Mutation; HGNC; HGNC:1773; CDK4; Simple; p.Arg24Cys (c.70C>T); ClinVar=VCV000016928; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Pro753Ser (c.2257C>T); ClinVar=VCV000376081; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Thr167Ala (c.499A>G); ClinVar=VCV001686107; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.-57A>C (c.161A>C) (A161C); ClinVar=VCV000242210; Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Leu145Arg (c.434T>G); ClinVar=VCV001027110; Zygosity=Homozygous (from parent cell line).
Disease Cutaneous melanoma (NCIt: C3510)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0526 (SK-MEL-28)
Sex of cell Male
Age at sampling 51Y
Category Cancer cell line
STR profile Source(s): ESTDAB=ESTDAB-130; PubMed=18689700

Markers:
AmelogeninX,Y
CSF1PO10,12
D3S135816,18
D5S81811,13
D7S82010
D8S117913
D13S31711,12
D16S5399,12
D18S5112,16
D21S1128,29
FGA19
TH017
TPOX8,12
vWA16,19

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=16772116; DOI=10.1016/j.cancergencyto.2005.11.016
Jason S. White, Robert L. Becker, Ian W. McLean, Alison E. Director-Myska, Joginder Nath;
Molecular cytogenetic evaluation of 10 uveal melanoma cell lines.
Cancer Genet. Cytogenet. 168:11-21(2006)

PubMed=18689700; DOI=10.1167/iovs.08-2324; PMCID=PMC2576485
Robert Folberg, ShriHari S. Kadkol, Shahar Frenkel, Klara Valyi-Nagy, Martine Johanna Jager, Jacob Pe'er, Andrew J. Maniotis;
Authenticating cell lines in ophthalmic research laboratories.
Invest. Ophthalmol. Vis. Sci. 49:4697-4701(2008)

PubMed=19340423; DOI=10.1007/s00262-009-0701-z; PMCID=PMC11030131
Rosa Mendez, Natalia Aptsiauri, Ana Del Campo, Isabel Maleno, Teresa Cabrera, Francisco Ruiz-Cabello Osuna, Federico Garrido, Angel Garcia-Lora;
HLA and melanoma: multiple alterations in HLA class I and II expression in human melanoma cell lines from ESTDAB cell bank.
Cancer Immunol. Immunother. 58:1507-1515(2009)

PubMed=22236444; DOI=10.1111/j.1755-148X.2012.00971.x; PMCID=PMC3288394
Klaus Georg Griewank, Xiao-Xing Yu, Jahan S. Khalili, Mustafa Mert Sozen, Katherine Stemke-Hale, Chantale Bernatchez, Seth Wardell, Boris C. Bastian, Scott E. Woodman;
Genetic and molecular characterization of uveal melanoma cell lines.
Pigment Cell Melanoma Res. 25:182-187(2012)

PubMed=23849826; DOI=10.1016/j.ejca.2013.06.011
Adriana Amaro, Valentina Mirisola, Giovanna Angelini, Alessandra Musso, Francesca Isabella Tosetti, Alessia Isabella Esposito, Patrizia Perri, Francesco Baldo Lanza, Francesca Nasciuti, Carlo Mosci, Roberto Puzone ...Show all 15 authors... , Sandra Salvi, Mauro Truini, Alessandro Poggi, Ulrich Pfeffer; Show fewer authors
Evidence of epidermal growth factor receptor expression in uveal melanoma: inhibition of epidermal growth factor-mediated signalling by gefitinib and cetuximab triggered antibody-dependent cellular cytotoxicity.
Eur. J. Cancer 49:3353-3365(2013)

Cross-references
Cell line databases/resources cancercelllines; CVCL_6938
ESTDAB; ESTDAB-130
Biological sample resources BioSample; SAMN06481120
Encyclopedic resources Wikidata; Q54931810
Polymorphism and mutation databases Cosmic; 1320370
Cosmic; 1628410
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number35