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Cellosaurus WI-L2-729HF2 (CVCL_6741)

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Cell line name WI-L2-729HF2
Synonyms WI-L2-729-HF2; WI-L2-729 HF2; 729-HF2; UC729-HF2; UC729HF2; HF2
Accession CVCL_6741
Resource Identification Initiative To cite this cell line use: WI-L2-729HF2 (RRID:CVCL_6741)
Comments Group: Hybridoma fusion partner cell line.
Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8062.
Population: Caucasian.
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Anecdotal: Because this cell line was deposited at ATCC on April 2, 1981 and the patent describing it was filled one week earlier (March, 26), the patent was first rejected. This decision was later reversed in a ruling that changed the deposition rules (DOI=10.1038/nbt1185-971).
Misspelling: W1-L2-729-HF2; PubMed=3101287.
Derived from site: In situ; Spleen; UBERON=UBERON_0002106.
Sequence variations
  • Mutation; HGNC; HGNC:11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
Disease Hereditary spherocytosis (NCIt: C97074)
Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D039 (UC 729-6)
Children:
CVCL_B6E2 (HD7/1A1.15.24)CVCL_2488 (HF2x653)CVCL_A6HX (HH09A2)
CVCL_A6HY (HH09A3)CVCL_E3ZG (HR78)CVCL_E3ZH (HR89)
Sex of cell Male
Age at sampling 5Y
Category Transformed cell line
STR profile Source(s): ATCC=CRL-8062

Markers:
AmelogeninX,Y
CSF1PO11,12
D5S81812,13
D7S8209,11
D13S31711
D16S53911,12
TH018,9.3
TPOX8,11
vWA17,20

Run an STR similarity search on this cell line
Web pages Info; -; -; https://digitalcommons.law.uw.edu/wlr/vol61/iss4/12
Publications

PubMed=6350451; DOI=10.4049/jimmunol.131.3.1201
Paul G. Abrams, James A. Knost, Gregory Clarke, Shannon Wilburn, Robert K. Oldham, Kenneth Alan Foon;
Determination of the optimal human cell lines for development of human hybridomas.
J. Immunol. 131:1201-1204(1983)

PubMed=6699402; DOI=10.4049/jimmunol.132.4.1798
Linda E. Strike, Bruce H. Devens, Robert L. Lundak;
Production of human-human hybridomas secreting antibody to sheep erythrocytes after in vitro immunization.
J. Immunol. 132:1798-1803(1984)

DOI=10.1038/nbt1185-971
Arthur Klausner;
Lundak ruling may ease deposit requirements.
Nat. Biotechnol. 3:971-971(1985)

Patent=US4594325
Robert L. Lundak;
High fusion frequency fusible lymphoblastoid cell line.
Patent number US4594325, 10-Jun-1986

PubMed=3101287; DOI=10.1111/j.1423-0410.1986.tb01955.x
Antony D. Lowe, Sheila M. Green, Douglas Voak, T. Gibson, Edwin Samuel Lennox;
A human-human monoclonal anti-D by direct fusion with a lymphoblastoid line.
Vox Sang. 51:212-216(1986)

PubMed=3365703
Thomas Borglum Kjeldsen, Birgitte Bruun Rasmussen, Carsten Rose, Jesper Zeuthen;
Human-human hybridomas and human monoclonal antibodies obtained by fusion of lymph node lymphocytes from breast cancer patients.
Cancer Res. 48:3208-3214(1988)

Cross-references
Cell line collections (Providers) ATCC; CRL-8062 - Discontinued
Cell line databases/resources CLO; CLO_0009603
Encyclopedic resources Wikidata; Q54994037
Entry history
Entry creation04-Apr-2012
Last entry update27-Nov-2025
Version number20