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Cellosaurus L5178Y TK+/- (clone 3.7.2C) (CVCL_6665)

[Text version]
Cell line name L5178Y TK+/- (clone 3.7.2C)
Synonyms L5178Y clone 3.7.2C; L5178Y TK+/- 3.7.2C; L5178Y TK+/-3.7.2c; TK+/- (clone 3.7.2C)
Accession CVCL_6665
Resource Identification Initiative To cite this cell line use: L5178Y TK+/- (clone 3.7.2C) (RRID:CVCL_6665)
Comments Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-9518.
Biotechnology: Used for mutation assays to predict the carcinogenic potential of chemicals. Allows the quantitation of forward mutations at the thymidine kinase (TK) locus. These TK heterozygous cells are treated with a range of doses of the test substance. The cells are incubated, washed free of tests substance and allowed time for the phenotypic expression of newly induced mutations (PubMed=1093012; PubMed=3416837; PubMed=4360720; PubMed=7329431).
Doubling time: ~10 hours (Note=Lot 02052008) (JCRB=JCRB0709.1).
Transformant: ChEBI; CHEBI_34342; 3-methylcholanthrene (3-MC; 20-methylcholanthrene; 20-MC; MCA).
Derived from site: In situ; Thymus; UBERON=UBERON_0002370.
Cell type: T-cell; CL=CL_0000084.
Disease Mouse thymic lymphoma (NCIt: C134997)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: DBA/2.
Hierarchy Parent: CVCL_4235 (L-5178-Y-S)
Children:
CVCL_4W65 (L5178Y TK+/- (clone 3.7.2C) (IVGT))
Sex of cell Female
Age at sampling 8M
Category Cancer cell line
Publications

PubMed=4360720; DOI=10.1289/ehp.7306119; PMCID=PMC1475522
Donald Clive;
Recent developments with the L5178Y TK heterozygote mutagen assay system.
Environ. Health Perspect. 6:119-125(1973)

PubMed=1093012; DOI=10.1016/0165-1161(75)90059-x
Donald Clive, Jo-Anna F.S. Spector;
Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells.
Mutat. Res. 31:17-29(1975)

PubMed=7329431; DOI=10.1016/0027-5107(81)90060-9
John C. Hozier, Jeffrey R. Sawyer, Martha May Moore, Barry Howard, Donald Clive;
Cytogenetic analysis of the L5178Y/TK+/- leads to TK-/- mouse lymphoma mutagenesis assay system.
Mutat. Res. 84:169-181(1981)

PubMed=4047078; DOI=10.1016/0165-1161(85)90064-0
John C. Hozier, Jeffrey R. Sawyer, Donald Clive, Martha May Moore;
Chromosome 11 aberrations in small colony L5178Y TK-/- mutants early in their clonal history.
Mutat. Res. 147:237-242(1985)

PubMed=4047079; DOI=10.1016/0165-1161(85)90065-2
Jeffrey R. Sawyer, Martha May Moore, Donald Clive, John C. Hozier;
Cytogenetic characterization of the L5178Y TK+/- 3.7.2C mouse lymphoma cell line.
Mutat. Res. 147:243-253(1985)

PubMed=27692294; DOI=10.1016/j.mrgentox.2016.08.001
Elisabeth Lorge, Martha May Moore, Julie Clements, Michael Rickard O'Donovan, Mick D. Fellows, Masamitsu Honma, Arihiro Kohara, Sheila Margaret Galloway, Michael J. Armstrong, Veronique Thybaud, Bala Bhaskar Gollapudi ...Show all 13 authors... , Marilyn J. Aardema, Jennifer Young Tanir; Show fewer authors
Standardized cell sources and recommendations for good cell culture practices in genotoxicity testing.
Mutat. Res. Genet. Toxicol. Environ. Mutagen. 809:1-15(2016)

Cross-references
Cell line collections (Providers) ATCC; CRL-9518
BCRC; 60112
BCRJ; 0140
JCRB; FDSC0053 - Discontinued
JCRB; JCRB0709.1
Cell line databases/resources CLO; CLO_0007196
CCRID; 1101MOU-PUMC000816
CCRID; 3101MOUTCM33
Encyclopedic resources Wikidata; Q54901039
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number19