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Cellosaurus CHLA-10 (CVCL_6583)

[Text version]
Cell line name CHLA-10
Synonyms CHLA10
Accession CVCL_6583
Resource Identification Initiative To cite this cell line use: CHLA-10 (RRID:CVCL_6583)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: Ewing Sarcoma Cell Line Atlas (ESCLA).
Part of: MD Anderson Cell Lines Project.
Part of: NCI Pediatric Preclinical Testing Program (PPTP) cell line panel.
Doubling time: 24 hours (PubMed=20922763); 32 hours (PubMed=24312454).
Omics: Genomics; ChIP-seq; FLI1.
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Transcriptomics; RNAseq; Single cell.
Derived from site: Metastatic; Thoracic lymph duct; UBERON=UBERON_0007644.
Sequence variations
  • Gene fusion; HGNC; HGNC:3508; EWSR1 + HGNC; HGNC:3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 1 fusion (Ex7/Ex6) (PubMed=36476851).
Disease Primitive neuroectodermal tumor (NCIt: C3716)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_M150 ! CHLA-9
Sex of cell Female
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): COG; PubMed=20922763; PubMed=24312454

Markers:
AmelogeninX
CSF1PO12
D2S133820
D3S135815
D5S81812
D7S8209,10
D8S117910,13
D13S31712
D16S53911
D18S5112,14
D19S43312,13
D21S1128,29
FGA23
TH017
TPOX11
vWA16

Run an STR similarity search on this cell line
Web pages Info; ESCLA; -; https://hgserver1.amc.nl/cgi-bin/r2/main.cgi?option=about_dscope
Info; MCLP; -; https://tcpaportal.org/mclp/
Provider; CCR; -; https://www.cccells.org/PDF_Files/Brain/CHLA-10%20Cell%20Line%20Data%20Sheet.pdf
Provider; CCR; -; https://www.cccells.org/tables/Ewings.php
Publications

PubMed=11212268
Thompson P.M., Maris J.M., Hogarty M.D., Seeger R.C., Reynolds C.P., Brodeur G.M., White P.S.
Homozygous deletion of CDKN2A (p16INK4a/p14ARF) but not within 1p36 or at other tumor suppressor loci in neuroblastoma.
Cancer Res. 61:679-686(2001)

PubMed=15289350; DOI=10.1158/0008-5472.CAN-04-0377
Batra S., Reynolds C.P., Maurer B.J.
Fenretinide cytotoxicity for Ewing's sarcoma and primitive neuroectodermal tumor cell lines is decreased by hypoxia and synergistically enhanced by ceramide modulators.
Cancer Res. 64:5415-5424(2004)

PubMed=20922763; DOI=10.1002/pbc.22801; PMCID=PMC3005554
Kang M.H., Smith M.A., Morton C.L., Keshelava N., Houghton P.J., Reynolds C.P.
National Cancer Institute pediatric preclinical testing program: model description for in vitro cytotoxicity testing.
Pediatr. Blood Cancer 56:239-249(2011)

PubMed=24312454; DOI=10.1371/journal.pone.0080060; PMCID=PMC3846563
May W.A., Grigoryan R.S., Keshelava N., Cabral D.J., Christensen L.L., Jenabi J., Ji L.-Y., Triche T.J., Lawlor E.R., Reynolds C.P.
Characterization and drug resistance patterns of Ewing's sarcoma family tumor cell lines.
PLoS ONE 8:e80060.1-e80060.10(2013)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074; PMCID=PMC4636476
Teicher B.A., Polley E.C., Kunkel M.W., Evans D., Silvers T.E., Delosh R.M., Laudeman J., Ogle C., Reinhart R., Selby M., Connelly J., Harris E., Monks A., Morris J.
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

DOI=10.5282/edoc.27750
Orth M.F.
Systematic multi-omics profiling of Ewing sarcoma cell lines.
Thesis PhD (2021); Ludwig Maximilians University of Munich; Munich; Germany

PubMed=36476851; DOI=10.1016/j.celrep.2022.111761; PMCID=PMC10333306
Orth M.F., Surdez D., Faehling T., Ehlers A.C., Marchetto A., Grossetete-Lalami S., Volckmann R., Zwijnenburg D.A., Gerke J.S., Zaidi S., Alonso J., Sastre A., Baulande S., Sill M., Cidre-Aranaz F., Ohmura S., Kirchner T., Hauck S.M., Reischl E., Gymrek M., Pfister S.M., Strauch K., Koster J., Delattre O., Grunewald T.G.P.
Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation.
Cell Rep. 41:111761.1-111761.28(2022)

Cross-references
Cell line databases/resources cancercelllines; CVCL_6583
Cell_Model_Passport; SIDM01542
DepMap; ACH-001029
Encyclopedic resources Wikidata; Q54812148
Gene expression databases GEO; GSM1676328
GEO; GSM1701625
GEO; GSM4368463
GEO; GSM5359634
GEO; GSM5359635
GEO; GSM5359636
GEO; GSM5359637
GEO; GSM5359638
GEO; GSM5359639
GEO; GSM5362864
GEO; GSM5362865
GEO; GSM5362866
GEO; GSM5362867
GEO; GSM5362868
GEO; GSM5362869
GEO; GSM5363941
GEO; GSM5363942
GEO; GSM5363943
GEO; GSM5363944
GEO; GSM5363945
Polymorphism and mutation databases Cosmic; 930038
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number22