Cellosaurus MB19tsA clone 2B2 (CVCL_6500)
| Cell line name | MB19tsA clone 2B2 |
|---|---|
| Synonyms | Mbeta19tsA clone 2B2; Mbeta19tsA, clone 2B2 |
| Accession | CVCL_6500 |
| Resource Identification Initiative | To cite this cell line use: MB19tsA clone 2B2 (RRID:CVCL_6500) |
| Comments | Characteristics: Deficient in base excision repair due to homozygous deletion of the promoter and exon 1 of polB. The deletion of polB is mediated by the Cre-loxP recombination. This deletion event is reversible and therefore this cell line is ideal for site-specific recombination using the Cre-lox system (ATCC=CRL-2308). Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58). Derived from site: In situ; Embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse) (NCBI Taxonomy: 10090) |
| Sex of cell | Sex unspecified |
| Age at sampling | 10-11FD |
| Category | Transformed cell line |
| Publications | PubMed=8538772; DOI=10.1038/379183a0 |
| Cross-references | |
| Cell line collections (Providers) | ATCC; CRL-2308 |
| Cell line databases/resources | CLO; CLO_0007554 |
| Encyclopedic resources | Wikidata; Q54904103 |
| Entry history | |
| Entry creation | 04-Apr-2012 |
| Last entry update | 14-Aug-2025 |
| Version number | 15 |