ID   MB19tsA clone 2B2
AC   CVCL_6500
SY   Mbeta19tsA clone 2B2; Mbeta19tsA, clone 2B2
DR   CLO; CLO_0007554
DR   ATCC; CRL-2308
DR   Wikidata; Q54904103
RX   PubMed=8538772;
CC   Characteristics: Deficient in base excision repair due to homozygous deletion of the promoter and exon 1 of polB. The deletion of polB is mediated by the Cre-loxP recombination. This deletion event is reversible and therefore this cell line is ideal for site-specific recombination using the Cre-lox system (ATCC=CRL-2308).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Sex unspecified
AG   10-11FD
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 14-08-25; Version: 15
//
RX   PubMed=8538772; DOI=10.1038/379183a0;
RA   Sobol, Robert William
RA   Horton, Julie K.
RA   Kuhn, Ralf
RA   Gu, Hua
RA   Singhal, Rakesh K.
RA   Prasad, Rajendra
RA   Rajewsky, Klaus
RA   Wilson, Samuel H. Jr.
RT   "Requirement of mammalian DNA polymerase-beta in base-excision
RT   repair.";
RL   Nature 379:183-186(1996).
//