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Cellosaurus RTgill-W1 (CVCL_6441)

[Text version]
Cell line name RTgill-W1
Synonyms RTGill-W1; RT-gill-W1; RT-gill W1; RTgillW1; RTG-W1; Rainbow Trout gill-Waterloo 1
Accession CVCL_6441
Resource Identification Initiative To cite this cell line use: RTgill-W1 (RRID:CVCL_6441)
Comments Group: Fish cell line.
Biotechnology: Used in the ISO 21115:2019 assay for the determination of acute toxicity of water samples.
Biotechnology: Used in the Organisation for Economic Co-operation and Development (OECD) fish cell line acute toxicity test method 249 (DOI=10.1787/c66d5190-en).
Characteristics: Used for acute toxicity tests on chemicals.
Doubling time: ~10-12 days (DSMZ=ACC-899).
Derived from site: In situ; Gill; UBERON=UBERON_0002535.
Cell type: Epithelial cell; CL=CL_0000066.
Species of origin Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) (NCBI Taxonomy: 8022)
Hierarchy Children:
CVCL_IW82 (RTgill-W1-pf)
Sex of cell Sex unspecified
Age at sampling Juvenile
Category Spontaneously immortalized cell line
Web pages https://aquatox-solutions.ch/wp-content/uploads/2019/04/RTgill-W1-Cell-Line-Assay.pdf

Bols N.C., Barlian A., Chirino-Trejo M., Caldwell S.J., Goegan P., Lee L.E.J.
Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills.
J. Fish Dis. 17:601-611(1994)

PubMed=19002917; DOI=10.1023/A:1020516804173
Perry G.M.L., McDonald G.J., Ferguson M.M., Ganassin R.C., Bols N.C.
Characterization of rainbow trout cell lines using microsatellite DNA profiling.
Cytotechnology 37:143-151(2001)

Bols N.C., Dayeh V.R., Lee L.E.J., Schirmer K.
Use of fish cell lines in the toxicology and ecotoxicology of fish Piscine cell lines in environmental toxicology.
(In) Biochemistry and molecular biology of fishes, Vol. 6; Mommsen T.P., Moon T.W. (eds.); pp.43-84; Elsevier; Amsterdam (2005)

PubMed=19184248; DOI=10.1007/s11626-008-9173-2
Lee L.E.J., Dayeh V.R., Schirmer K., Bols N.C.
Applications and potential uses of fish gill cell lines: examples with RTgill-W1.
In Vitro Cell. Dev. Biol. Anim. 45:127-134(2009)

PubMed=20420918; DOI=10.1016/j.fsi.2010.04.007
Kawano A., Kales S.C., Fujiki K., DeWitte-Orr S.J., Dixon B., Lee L.E.J., Bols N.C.
A comparison of rainbow trout cell lines for their expression of the major histocompatibility complex genes and the induction of beta-2-microglobulin by dsRNA.
Fish Shellfish Immunol. 29:312-318(2010)

PubMed=22281180; DOI=10.1016/j.tiv.2012.01.001
Kienzler A., Tronchere X., Devaux A., Bony S.
Assessment of RTG-W1, RTL-W1, and PLHC-1 fish cell lines for genotoxicity testing of environmental pollutants by means of a Fpg-modified comet assay.
Toxicol. In Vitro 26:500-510(2012)

PubMed=24357037; DOI=10.1007/s11626-013-9720-3
Vo N.T.K., Bufalino M.R., Hartlen K.D., Kitaev V., Lee L.E.J.
Cytotoxicity evaluation of silica nanoparticles using fish cell lines.
In Vitro Cell. Dev. Biol. Anim. 50:427-438(2014)

Yue Y.
Interaction of silver nanoparticles (AgNP) with rainbow trout gill cell lines.
Thesis PhD (2015), Ecole Polytechnique Federale de Lausanne, Switzerland

PubMed=28374170; DOI=10.1007/s11626-017-0142-5
Bols N.C., Pham P.H., Dayeh V.R., Lee L.E.J.
Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout.
In Vitro Cell. Dev. Biol. Anim. 53:383-405(2017)

PubMed=30825313; DOI=10.1093/toxsci/kfz057
Fischer M., Belanger S.E., Berckmans P., Bernhard M.J., Blaha L., Coman Schmid D.E., Dyer S.D., Haupt T., Hermens J.L.M., Hultman M.T., Laue H., Lillicrap A.D., Mlnarikova M., Natsch A., Novak J., Sinnige T.L., Tollefsen K.E., von Niederhausern V., Witters H., Zupanic A., Schirmer K.
Repeatability and reproducibility of the RTgill-W1 cell line assay for predicting fish acute toxicity.
Toxicol. Sci. 169:353-364(2019)

PubMed=33053677; DOI=10.3390/pathogens9100833
Pham P.H., Misk E., Papazotos F., Jones G., Polinski M.P., Contador E., Russell S., Garver K.A., Lumsden J.S., Bols N.C.
Screening of fish cell lines for piscine orthoreovirus-1 (PRV-1) amplification: identification of the non-supportive PRV-1 invitrome.
Pathogens 9:833.1-833.25(2020)

Test No. 249: fish cell line acute toxicity -- the RTgill-W1 cell line assay.
(In) OECD guidelines for the testing of chemicals, Section 2; pp.1-51; Paris (2021)

Rebl H., Bergemann C., Rakers S., Nebe B., Rebl A.
Plasma treatment of fish cells: the importance of defining cell culture conditions in comparative studies.
Appl. Sci. 11:2534.1-2534.16(2021)

Cell line collections (Providers) ATCC; CRL-2523
BCRJ; 0362 - Discontinued
Cell line databases/resources CLO; CLO_0008896
DSMZCellDive; ACC-899
FCS-free; 159-12-290-1-16-3
Encyclopedic resources Wikidata; Q54951402
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number25