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Cellosaurus RTgill-W1 (CVCL_6441)

[Text version]
Cell line name RTgill-W1
Synonyms RTGill-W1; RT-gill-W1; RT-gill W1; RTgillW1; RTG-W1; Rainbow Trout gill-Waterloo 1
Accession CVCL_6441
Resource Identification Initiative To cite this cell line use: RTgill-W1 (RRID:CVCL_6441)
Comments Group: Fish cell line.
Biotechnology: Used in the ISO 21115:2019 assay for the determination of acute toxicity of water samples (CelloPub=CLPUB00786).
Biotechnology: Used in the Organisation for Economic Co-operation and Development (OECD) fish cell line acute toxicity test method 249 (DOI=10.1787/c66d5190-en).
Biotechnology: Used in an assay for ichthyotoxic assessment of red tide phytoplankton (DOI=10.1016/j.hal.2011.01.002).
Characteristics: Used for acute toxicity tests on chemicals.
Doubling time: ~10-12 days (DSMZ=ACC-899); 3.71 +- 0.56 days (DOI=10.1016/j.namjnl.2025.100008).
Derived from site: In situ; Gill; UBERON=UBERON_0002535.
Cell type: Epithelial cell; CL=CL_0000066.
Species of origin Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) (NCBI Taxonomy: 8022)
Hierarchy Children:
CVCL_IW82 (RTgill-W1-pf)CVCL_E7CD (RTgill-W1-sf)
Sex of cell Sex unspecified
Age at sampling Juvenile
Category Spontaneously immortalized cell line
Web pages Info; -; -; https://aquatox-solutions.ch/wp-content/uploads/2019/04/RTgill-W1-Cell-Line-Assay.pdf
Publications

DOI=10.1111/j.1365-2761.1994.tb00258.x
Niels C. Bols, Anggraini Barlian, Manuel Chirino-Trejo, Sarah J. Caldwell, Patrick Goegan, Lucy E.J. Lee;
Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills.
J. Fish Dis. 17:601-611(1994)

PubMed=19002917; DOI=10.1023/A:1020516804173; PMCID=PMC3449788
Guy M.L. Perry, Gordon J. McDonald, Moira M. Ferguson, Rosemarie Catherine Ganassin, Niels C. Bols;
Characterization of rainbow trout cell lines using microsatellite DNA profiling.
Cytotechnology 37:143-151(2001)

DOI=10.1016/S1873-0140(05)80005-0
Niels C. Bols, Vivian R. Dayeh, Lucy E.J. Lee, Kristin Schirmer;
Use of fish cell lines in the toxicology and ecotoxicology of fish Piscine cell lines in environmental toxicology.
(In book chapter) Biochemistry and molecular biology of fishes, Vol. 6; Mommsen, Thomas P. & Moon, Thomas W. (eds.); pp.43-84; Elsevier; Amsterdam; Netherlands (2005)

PubMed=19184248; DOI=10.1007/s11626-008-9173-2
Lucy E.J. Lee, Vivian R. Dayeh, Kristin Schirmer, Niels C. Bols;
Applications and potential uses of fish gill cell lines: examples with RTgill-W1.
In Vitro Cell. Dev. Biol. Anim. 45:127-134(2009)

PubMed=20420918; DOI=10.1016/j.fsi.2010.04.007
Atsushi Kawano, Steve C. Kales, Kazuhiro Fujiki, Stephanie J. DeWitte-Orr, Brian Dixon, Lucy E.J. Lee, Niels C. Bols;
A comparison of rainbow trout cell lines for their expression of the major histocompatibility complex genes and the induction of beta-2-microglobulin by dsRNA.
Fish Shellfish Immunol. 29:312-318(2010)

DOI=10.1016/j.hal.2011.01.002
Juan Jose Dorantes-Aranda, T. David Waite, Aurelie Godrant, Andrew L. Rose, Cesar Dario Tovar Lopez, Gregory M. Woods, Gustaaf M. Hallegraeff;
Novel application of a fish gill cell line assay to assess ichthyotoxicity of harmful marine microalgae.
Harmful Algae 10:366-373(2011)

PubMed=22281180; DOI=10.1016/j.tiv.2012.01.001
Aude Kienzler, Xavier Tronchere, Alain Devaux, Sylvie Bony;
Assessment of RTG-W1, RTL-W1, and PLHC-1 fish cell lines for genotoxicity testing of environmental pollutants by means of a Fpg-modified comet assay.
Toxicol. In Vitro 26:500-510(2012)

PubMed=24357037; DOI=10.1007/s11626-013-9720-3
Nguyen T.K. Vo, Mary Rose Bufalino, Kurtis D. Hartlen, Vladimir Kitaev, Lucy E.J. Lee;
Cytotoxicity evaluation of silica nanoparticles using fish cell lines.
In Vitro Cell. Dev. Biol. Anim. 50:427-438(2014)

DOI=10.5075/epfl-thesis-6698
Yang Yue;
Interaction of silver nanoparticles (AgNP) with rainbow trout gill cell lines.
Thesis PhD (2015); Ecole Polytechnique Federale de Lausanne; Lausanne; Switzerland

PubMed=28374170; DOI=10.1007/s11626-017-0142-5
Niels C. Bols, Phuc H. Pham, Vivian R. Dayeh, Lucy E.J. Lee;
Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout.
In Vitro Cell. Dev. Biol. Anim. 53:383-405(2017)

CLPUB00786
International Organization for Standardization (ISO)
Water quality -- Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1).
(In technical document) ISO 21115:2019; pp.1-38; International Organization for Standardization; Geneva; Switzerland (2019)

PubMed=30825313; DOI=10.1093/toxsci/kfz057; PMCID=PMC6542334
Melanie Fischer, Scott E. Belanger, Pascale Berckmans, Mary J. Bernhard, Ludek Blaha, Diana E. Coman Schmid, Scott D. Dyer, Tina Haupt, Joop L.M. Hermens, Maria Therese Hultman, Heike Laue ...Show all 21 authors... , Adam David Lillicrap, Marie Mlnarikova, Andreas Natsch, Jiri Novak, Theo L. Sinnige, Knut Erik Tollefsen, Valentin von Niederhausern, Hilda Witters, Anze Zupanic, Kristin Schirmer; Show fewer authors
Repeatability and reproducibility of the RTgill-W1 cell line assay for predicting fish acute toxicity.
Toxicol. Sci. 169:353-364(2019)

PubMed=33053677; DOI=10.3390/pathogens9100833; PMCID=PMC7601784
Phuc H. Pham, Ehab Misk, Fotini Papazotos, Ginny Jones, Mark P. Polinski, Elena Contador, Spencer Russell, Kyle A. Garver, John S. Lumsden, Niels C. Bols;
Screening of fish cell lines for piscine orthoreovirus-1 (PRV-1) amplification: identification of the non-supportive PRV-1 invitrome.
Pathogens 9:833.1-833.25(2020)

DOI=10.1787/c66d5190-en
Organisation for Economic Co-operation and Development (OECD)
Test No. 249: fish cell line acute toxicity -- the RTgill-W1 cell line assay.
(In technical document) OECD guidelines for the testing of chemicals, Section 2; pp.1-51; OECD Publishing; Paris; France (2021)

DOI=10.3390/app11062534
Henrike Rebl, Claudia Bergemann, Sebastian Rakers, Barbara Nebe, Alexander Rebl;
Plasma treatment of fish cells: the importance of defining cell culture conditions in comparative studies.
Appl. Sci. 11:2534.1-2534.16(2021)

DOI=10.1016/j.namjnl.2025.100008
Barbara S. Jozef, Zhao-Rui Zhang, Hans-Michael Kaltenbach, Kristin Schirmer;
A systematic approach towards long-term, serum-free cultivation of fish cells with the RTgill-W1 cell line as example.
NAM J. 1:100008.1-100008.13(2025)

Cross-references
Cell line collections (Providers) ATCC; CRL-2523
BCRJ; 0362 - Discontinued
DSMZ; ACC-899
Cell line databases/resources CLO; CLO_0008896
DSMZCellDive; ACC-899
FCS-free; 159-12-290-1-16-3
Encyclopedic resources Wikidata; Q54951402
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number27