ID   RTgill-W1
AC   CVCL_6441
SY   RTGill-W1; RT-gill-W1; RT-gill W1; RTgillW1; RTG-W1; Rainbow Trout gill-Waterloo 1
DR   CLO; CLO_0008896
DR   ATCC; CRL-2523
DR   BCRJ; 0362
DR   DSMZ; ACC-899
DR   DSMZCellDive; ACC-899
DR   FCS-free; 159-12-290-1-16-3
DR   Wikidata; Q54951402
RX   CelloPub=CLPUB00786;
RX   DOI=10.1016/j.hal.2011.01.002;
RX   DOI=10.1016/j.namjnl.2025.100008;
RX   DOI=10.1016/S1873-0140(05)80005-0;
RX   DOI=10.1111/j.1365-2761.1994.tb00258.x;
RX   DOI=10.1787/c66d5190-en;
RX   DOI=10.3390/app11062534;
RX   DOI=10.5075/epfl-thesis-6698;
RX   PubMed=19002917;
RX   PubMed=19184248;
RX   PubMed=20420918;
RX   PubMed=22281180;
RX   PubMed=24357037;
RX   PubMed=28374170;
RX   PubMed=30825313;
RX   PubMed=33053677;
WW   Info; -; -; https://aquatox-solutions.ch/wp-content/uploads/2019/04/RTgill-W1-Cell-Line-Assay.pdf
CC   Group: Fish cell line.
CC   Biotechnology: Used in the ISO 21115:2019 assay for the determination of acute toxicity of water samples (CelloPub=CLPUB00786).
CC   Biotechnology: Used in the Organisation for Economic Co-operation and Development (OECD) fish cell line acute toxicity test method 249 (DOI=10.1787/c66d5190-en).
CC   Biotechnology: Used in an assay for ichthyotoxic assessment of red tide phytoplankton (DOI=10.1016/j.hal.2011.01.002).
CC   Characteristics: Used for acute toxicity tests on chemicals.
CC   Doubling time: ~10-12 days (DSMZ=ACC-899); 3.71 +- 0.56 days (DOI=10.1016/j.namjnl.2025.100008).
CC   Discontinued: BCRJ; 0362; true.
CC   Derived from site: In situ; Gill; UBERON=UBERON_0002535.
CC   Cell type: Epithelial cell; CL=CL_0000066.
OX   NCBI_TaxID=8022; ! Oncorhynchus mykiss (Rainbow trout)
SX   Sex unspecified
AG   Juvenile
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 27
//
RX   CelloPub=CLPUB00786;
RG   International Organization for Standardization (ISO);
RT   "Water quality -- Determination of acute toxicity of water samples and
RT   chemicals to a fish gill cell line (RTgill-W1).";
RL   (In technical document) ISO 21115:2019; pp.1-38; International Organization for Standardization; Geneva; Switzerland (2019).
//
RX   DOI=10.1016/j.hal.2011.01.002;
RA   Dorantes-Aranda J.J., Waite T.D., Godrant A., Rose A.L.,
RA   Tovar Lopez C.D., Woods G.M., Hallegraeff G.M.;
RT   "Novel application of a fish gill cell line assay to assess
RT   ichthyotoxicity of harmful marine microalgae.";
RL   Harmful Algae 10:366-373(2011).
//
RX   DOI=10.1016/j.namjnl.2025.100008;
RA   Jozef B.S., Zhang Z.-R., Kaltenbach H.-M., Schirmer K.;
RT   "A systematic approach towards long-term, serum-free cultivation of
RT   fish cells with the RTgill-W1 cell line as example.";
RL   NAM J. 1:100008.1-100008.13(2025).
//
RX   DOI=10.1016/S1873-0140(05)80005-0;
RA   Bols N.C., Dayeh V.R., Lee L.E.J., Schirmer K.;
RT   "Use of fish cell lines in the toxicology and ecotoxicology of fish.
RT   Piscine cell lines in environmental toxicology.";
RL   (In book chapter) Biochemistry and molecular biology of fishes, Vol. 6; Mommsen T.P., Moon T.W. (eds.); pp.43-84; Elsevier; Amsterdam; Netherlands (2005).
//
RX   DOI=10.1111/j.1365-2761.1994.tb00258.x;
RA   Bols N.C., Barlian A., Chirino-Trejo M., Caldwell S.J., Goegan P.,
RA   Lee L.E.J.;
RT   "Development of a cell line from primary cultures of rainbow trout,
RT   Oncorhynchus mykiss (Walbaum), gills.";
RL   J. Fish Dis. 17:601-611(1994).
//
RX   DOI=10.1787/c66d5190-en;
RG   Organisation for Economic Co-operation and Development (OECD);
RT   "Test No. 249: fish cell line acute toxicity -- the RTgill-W1 cell line assay.";
RL   (In technical document) OECD guidelines for the testing of chemicals, Section 2; pp.1-51; OECD Publishing; Paris; France (2021).
//
RX   DOI=10.3390/app11062534;
RA   Rebl H., Bergemann C., Rakers S., Nebe B., Rebl A.;
RT   "Plasma treatment of fish cells: the importance of defining cell
RT   culture conditions in comparative studies.";
RL   Appl. Sci. 11:2534.1-2534.16(2021).
//
RX   DOI=10.5075/epfl-thesis-6698;
RA   Yue Y.;
RT   "Interaction of silver nanoparticles (AgNP) with rainbow trout gill
RT   cell lines.";
RL   Thesis PhD (2015); Ecole Polytechnique Federale de Lausanne; Lausanne; Switzerland.
//
RX   PubMed=19002917; DOI=10.1023/A:1020516804173; PMCID=PMC3449788;
RA   Perry G.M.L., McDonald G.J., Ferguson M.M., Ganassin R.C., Bols N.C.;
RT   "Characterization of rainbow trout cell lines using microsatellite DNA
RT   profiling.";
RL   Cytotechnology 37:143-151(2001).
//
RX   PubMed=19184248; DOI=10.1007/s11626-008-9173-2;
RA   Lee L.E.J., Dayeh V.R., Schirmer K., Bols N.C.;
RT   "Applications and potential uses of fish gill cell lines: examples
RT   with RTgill-W1.";
RL   In Vitro Cell. Dev. Biol. Anim. 45:127-134(2009).
//
RX   PubMed=20420918; DOI=10.1016/j.fsi.2010.04.007;
RA   Kawano A., Kales S.C., Fujiki K., DeWitte-Orr S.J., Dixon B.,
RA   Lee L.E.J., Bols N.C.;
RT   "A comparison of rainbow trout cell lines for their expression of the
RT   major histocompatibility complex genes and the induction of
RT   beta-2-microglobulin by dsRNA.";
RL   Fish Shellfish Immunol. 29:312-318(2010).
//
RX   PubMed=22281180; DOI=10.1016/j.tiv.2012.01.001;
RA   Kienzler A., Tronchere X., Devaux A., Bony S.;
RT   "Assessment of RTG-W1, RTL-W1, and PLHC-1 fish cell lines for
RT   genotoxicity testing of environmental pollutants by means of a
RT   Fpg-modified comet assay.";
RL   Toxicol. In Vitro 26:500-510(2012).
//
RX   PubMed=24357037; DOI=10.1007/s11626-013-9720-3;
RA   Vo N.T.K., Bufalino M.R., Hartlen K.D., Kitaev V., Lee L.E.J.;
RT   "Cytotoxicity evaluation of silica nanoparticles using fish cell
RT   lines.";
RL   In Vitro Cell. Dev. Biol. Anim. 50:427-438(2014).
//
RX   PubMed=28374170; DOI=10.1007/s11626-017-0142-5;
RA   Bols N.C., Pham P.H., Dayeh V.R., Lee L.E.J.;
RT   "Invitromatics, invitrome, and invitroomics: introduction of three new
RT   terms for in vitro biology and illustration of their use with the cell
RT   lines from rainbow trout.";
RL   In Vitro Cell. Dev. Biol. Anim. 53:383-405(2017).
//
RX   PubMed=30825313; DOI=10.1093/toxsci/kfz057; PMCID=PMC6542334;
RA   Fischer M., Belanger S.E., Berckmans P., Bernhard M.J., Blaha L.,
RA   Coman Schmid D.E., Dyer S.D., Haupt T., Hermens J.L.M., Hultman M.T.,
RA   Laue H., Lillicrap A.D., Mlnarikova M., Natsch A., Novak J.,
RA   Sinnige T.L., Tollefsen K.E., von Niederhausern V., Witters H.,
RA   Zupanic A., Schirmer K.;
RT   "Repeatability and reproducibility of the RTgill-W1 cell line assay
RT   for predicting fish acute toxicity.";
RL   Toxicol. Sci. 169:353-364(2019).
//
RX   PubMed=33053677; DOI=10.3390/pathogens9100833; PMCID=PMC7601784;
RA   Pham P.H., Misk E., Papazotos F., Jones G., Polinski M.P.,
RA   Contador E., Russell S., Garver K.A., Lumsden J.S., Bols N.C.;
RT   "Screening of fish cell lines for piscine orthoreovirus-1 (PRV-1)
RT   amplification: identification of the non-supportive PRV-1 invitrome.";
RL   Pathogens 9:833.1-833.25(2020).
//