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Cellosaurus DRO (CVCL_6286)

[Text version]
Cell line name DRO
Synonyms DRO-90-1; DRO 90-1; DRO90-1; DRO-90; RO 90-D-1; UCLA RO 90-D-1
Accession CVCL_6286
Resource Identification Initiative To cite this cell line use: DRO (RRID:CVCL_6286)
Comments Problematic cell line: Contaminated. Shown to be a A-375 derivative (PubMed=18713817; PubMed=20143388; PubMed=21868764). Originally thought to originate from a thyroid gland anaplastic carcinoma.
From: Juillard G.J.-F.; University of California, Los Angeles; Los Angeles; USA.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00188.
Population: Caucasian.
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0132 (A-375)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): PubMed=18713817; PubMed=21868764

Markers:
AmelogeninX
CSF1PO11,12
D3S135815,17
D5S81812
D7S8209
D8S117911,14
D13S31711,14
D16S5399
D18S5112,17
D21S1129,30
FGA20,23
TH018
TPOX8,10
vWA16,17

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=8871538; DOI=10.1007/BF00128960
Boghaert E.R., Ain K.B., Taylor K.D., Greenberg V.L., Fowler C., Zimmer S.G.
Quantitative and qualitative differences in growth, invasion and lung colonization of an anaplastic and a papillary human thyroid cancer cell line in vitro and in vivo.
Clin. Exp. Metastasis 14:440-450(1996)

PubMed=18713817; DOI=10.1210/jc.2008-1102
Schweppe R.E., Klopper J.P., Korch C.T., Pugazhenthi U., Benezra M., Knauf J.A., Fagin J.A., Marlow L.A., Copland J.A. III, Smallridge R.C., Haugen B.R.
Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification.
J. Clin. Endocrinol. Metab. 93:4331-4341(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

Cross-references
Cell line databases/resources cancercelllines; CVCL_6286
Biological sample resources BioSample; SAMN03151608
BioSample; SAMN03151609
Encyclopedic resources Wikidata; Q54831419
Polymorphism and mutation databases Cosmic; 886551
Cosmic; 888278
Cosmic; 913249
Cosmic; 1155330
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number26