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Cellosaurus 661W (CVCL_6240)

[Text version]
Cell line name 661W
Synonyms 661w; 661 W
Accession CVCL_6240
Resource Identification Initiative To cite this cell line use: 661W (RRID:CVCL_6240)
Comments Doubling time: ~24 hours (PubMed=12407171).
Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen (Note=Under the control of the IRBP promoter).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Omics: Proteomics; 2DE-MS.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Eye, retina; UBERON=UBERON_0000966.
Cell type: Retinal cone cell; CL=CL_0000573.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6N transgenic.
Hierarchy Children:
CVCL_E9DQ (661W-A11)CVCL_4059 (RGC-5)
Sex of cell Male
Age at sampling Age unspecified
Category Transformed cell line
Publications

PubMed=11243878; DOI=10.1006/bbrc.2001.4501
Matthew J. Crawford, Raghu R. Krishnamoorthy, Victoria L. Rudick, Robert John Collier, Michael Kapin, Bharat Bhushan Aggarwal, Muayyad R. Al-Ubaidi, Neeraj Agarwal;
Bcl-2 overexpression protects photooxidative stress-induced apoptosis of photoreceptor cells via NF-kappaB preservation.
Biochem. Biophys. Res. Commun. 281:1304-1312(2001)

PubMed=12407171
Gearoid Tuohy, Sophia Millington-Ward, Paul F. Kenna, Peter Humphries, Gwyneth Jane Farrar;
Sensitivity of photoreceptor-derived cell line (661W) to baculoviral p35, Z-VAD.FMK, and Fas-associated death domain.
Invest. Ophthalmol. Vis. Sci. 43:3583-3589(2002)

PubMed=14985288; DOI=10.1167/iovs.03-1114; PMCID=PMC2937568
Elaine Tan, Xi-Qin Ding, Anisse Saadi, Neeraj Agarwal, Muna I. Naash, Muayyad R. Al-Ubaidi;
Expression of cone-photoreceptor-specific antigens in a cell line derived from retinal tumors in transgenic mice.
Invest. Ophthalmol. Vis. Sci. 45:764-768(2004)

PubMed=18188958; DOI=10.1007/978-0-387-74904-4_35
Muayyad R. Al-Ubaidi, Hiroyuki Matsumoto, Sadamu Kurono, Anil Singh;
Proteomics profiling of the cone photoreceptor cell line, 661W.
Adv. Exp. Med. Biol. 613:301-311(2008)

PubMed=23975727; DOI=10.1167/iovs.13-12085
Raghu R. Krishnamoorthy, Abbot F. Clark, Donald Daudt, Jamboor K. Vishwanatha, Thomas Yorio;
A forensic path to RGC-5 cell line identification: lessons learned.
Invest. Ophthalmol. Vis. Sci. 54:5712-5719(2013)

PubMed=26854824; DOI=10.1016/j.exer.2016.01.016; PMCID=PMC5024725
Bruce A. Pfeffer, Li-Bin Xu, Ned A. Porter, Sriganesh Ramachandra Rao, Steven J. Fliesler;
Differential cytotoxic effects of 7-dehydrocholesterol-derived oxysterols on cultured retina-derived cells: dependence on sterol structure, cell type, and density.
Exp. Eye Res. 145:297-316(2016)

PubMed=29203899; DOI=10.1038/s41598-017-17241-0; PMCID=PMC5715133
Zuberwasim Sayyad, Kapil Sirohi, Vegesna Radha, Ghanshyam Swarup;
661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.
Sci. Rep. 7:16855-16855(2017)

PubMed=31024622; DOI=10.3389/fgene.2019.00308; PMCID=PMC6459963
Gabrielle Wheway, Liliya Nazlamova, Dann Turner, Stephen Cross;
661W photoreceptor cell line as a cell model for studying retinal ciliopathies.
Front. Genet. 10:308.1-308.14(2019)

PubMed=31819341; PMCID=PMC6887793
Aida Sanchez-Bretano, Ting-Chung Suen, Kenkichi Baba, Jason P. DeBruyne, Gianluca Tosini;
Melatonin receptor heterodimerization in a photoreceptor-like cell line endogenously expressing melatonin receptors.
Mol. Vis. 25:791-799(2019)

PubMed=34208617; DOI=10.3390/ijms22126440; PMCID=PMC8235468
Li Huang, Meltem Kutluer, Elisa Adani, Antonella Comitato, Valeria Marigo;
New in vitro cellular model for molecular studies of retinitis pigmentosa.
Int. J. Mol. Sci. 22:6440.1-6440.12(2021)

PubMed=40170180; DOI=10.1186/s13578-025-01381-2; PMCID=PMC11959731
Alicia A. Brunet, Rebekah E. James, Petria Swanson, Livia Santos Carvalho;
A review of the 661W cell line as a tool to facilitate treatment development for retinal diseases.
Cell Biosci. 15:41.1-41.19(2025)

Cross-references
Cell line databases/resources Lonza; 336
Anatomy/cell type resources BTO; BTO_0001554
Chemistry resources ChEMBL-Cells; CHEMBL4483169
ChEMBL-Targets; CHEMBL4483223
PubChem_Cell_line; CVCL_6240
Encyclopedic resources Wikidata; Q54604409
Gene expression databases GEO; GSE119190
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number18