Cellosaurus J774-Dual (CVCL_5I75)
| Cell line name | J774-Dual |
|---|---|
| Accession | CVCL_5I75 |
| Resource Identification Initiative | To cite this cell line use: J774-Dual (RRID:CVCL_5I75) |
| Comments | Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites and 3 c-Rel binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE) (InvivoGen=j774d-nfis). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr. Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Genetic integration: Method=Transfection; Gene=Synthetic construct Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Derived from site: In situ; Ascites; UBERON=UBERON_0007795. Cell type: Macrophage; CL=CL_0000235. |
| Disease | Mouse reticulum cell sarcoma (NCIt: C134780) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c. |
| Hierarchy | Parent: CVCL_4770 (J774.1) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | InvivoGen; j774d-nfis |
| Encyclopedic resources | Wikidata; Q54898484 |
| Entry history | |
| Entry creation | 14-Dec-2015 |
| Last entry update | 14-Aug-2025 |
| Version number | 13 |