ID   J774-Dual
AC   CVCL_5I75
DR   InvivoGen; j774d-nfis
DR   Wikidata; Q54898484
CC   Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites and 3 c-Rel binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE) (InvivoGen=j774d-nfis).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Genetic integration: Method=Transfection; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
CC   Derived from site: In situ; Ascites; UBERON=UBERON_0007795.
CC   Cell type: Macrophage; CL=CL_0000235.
CC   Breed/subspecies: BALB/c.
DI   NCIt; C134780; Mouse reticulum cell sarcoma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_4770 ! J774.1
SX   Female
AG   Adult
CA   Cancer cell line
DT   Created: 14-12-15; Last updated: 10-04-25; Version: 12
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