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Cellosaurus KM12-L4 (CVCL_5946)

[Text version]
Cell line name KM12-L4
Synonyms KM12L4; Km12L4; KM12 L4; Km 12 L4
Accession CVCL_5946
Resource Identification Initiative To cite this cell line use: KM12-L4 (RRID:CVCL_5946)
Comments Population: Caucasian.
Characteristics: Highly liver metastatic. Established after four passages into the cecum of nude mice (PubMed=3349467).
Microsatellite instability: Instable (MSI) (PubMed=25926053).
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Gene fusion; HGNC; HGNC:8031; NTRK1 + HGNC; HGNC:12012; TPM3; Name(s)=TPM3-NTRK1 (from parent cell line).
  • Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Asn1819fs*7 (c.5454_5455insA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Asn1784Hisfs*7 (c.5350_5351delAA); ClinVar=VCV000037959; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Gly129Ter (c.385G>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA); ClinVar=VCV000092828; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Val73Trpfs*50 (c.216delC) (p.P72fs); ClinVar=VCV000428897; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Arg (c.536A>G); ClinVar=VCV000376606; Zygosity=Heterozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1331 (KM12)
Children:
CVCL_W220 (KM12-L4A)CVCL_5920 (KM12-OxR)CVCL_AU12 (KM12L4/OXR)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): PubMed=25926053

Markers:
AmelogeninX
CSF1PO10,12
D5S81811
D7S8208,9
D13S31713,15
D16S53911,12
D21S1127,34
TH0110
TPOX11,12
vWA16,17

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Publications

PubMed=2846163
Kiyoshi Morikawa, Shirley M. Walker, Motowo Nakajima, Sen Pathak, John Milburn Jessup, Isaiah Joshua Fidler;
Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice.
Cancer Res. 48:6863-6871(1988)

PubMed=3349467
Kiyoshi Morikawa, Shirley M. Walker, John Milburn Jessup, Isaiah Joshua Fidler;
In vivo selection of highly metastatic cells from surgical specimens of different primary human colon carcinomas implanted into nude mice.
Cancer Res. 48:1943-1948(1988)

PubMed=8909255; PMCID=PMC1865285
Douglas J. Goetz, Han Ding, William J. Atkinson, Gloria Vachino, Raymond T. Camphausen, Dale A. Cumming, Francis W. Luscinskas;
A human colon carcinoma cell line exhibits adhesive interactions with P-selectin under fluid flow via a PSGL-1-independent mechanism.
Am. J. Pathol. 149:1661-1673(1996)

PubMed=10091941; DOI=10.1023/A:1006537609469
Yoshifumi Nakayama, Keisuke Okazaki, Kazunori Shibao, Tatsuhiko Sako, Keiji Hirata, Naoki Nagata, Michihiko Kuwano, Hideaki Itoh;
Alterative expression of the collagenase and adhesion molecules in the highly metastatic clones of human colonic cancer cell lines.
Clin. Exp. Metastasis 16:461-469(1998)

PubMed=25926053; DOI=10.1038/ncomms8002
Enzo Medico, Mariangela Russo, Gabriele Picco, Carlotta Cancelliere, Emanuele Valtorta, Giorgio Corti, Michela Buscarino, Claudio Isella, Simona Lamba, Barbara Martinoglio, Silvio Veronese ...Show all 19 authors... , Salvatore Siena, Andrea Sartore-Bianchi, Marco Beccuti, Marcella Mottolese, Michael Linnebacher, Francesca Cordero, Federica Di Nicolantonio, Alberto Bardelli; Show fewer authors
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

Cross-references
Cell line collections (Providers) KCLB; 80014
Cell line databases/resources cancercelllines; CVCL_5946
Encyclopedic resources Wikidata; Q54900057
Gene expression databases GEO; GSM1448187
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number23