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Cellosaurus LX-2 (CVCL_5792)

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Cell line name LX-2
Synonyms Lieming Xu-2
Accession CVCL_5792
Resource Identification Initiative To cite this cell line use: LX-2 (RRID:CVCL_5792)
Comments Characteristics: Able to grow under reduced serum conditions (1% FBS) (PubMed=15591520).
Doubling time: 48-60 hours (Hysigen=TCH-C391); ~3-4 days (PubMed=15591520); 53.3 +- 2.3 hours (PubMed=21930125).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRSV-T).
Omics: Proteomics; Quantitative.
Omics: Proteomics; Subcellular; Extracellular matrix.
Anecdotal: Cell line name is derived from the name of the first author of the original paper.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatic stellate cell; CL=CL_0000632.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_5790 (LX-1 [Human HSC])
Children:
CVCL_E1LU (HyCyte LX-2 KO-hNGLY1)CVCL_F1RF (HyCyte LX-2 KO-hTPP1)CVCL_E3HV (HyCyte LX-2-Luc)
Sex of cell Male
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): Hysigen=TCH-C391; Millipore=SCC064; PubMed=24116068; Technion Genomics Center

Markers:
AmelogeninX,Y
CSF1PO10,12
D1S165612
D2S44111,14
D2S133817
D3S135813,15
D5S81811,12
D6S104311,12
D7S82011
D8S117913
D10S124813
D12S39115,17
D13S31711,13
D16S53913
D18S5112
D19S43313,15.2
D21S1128,31
D22S104516
DYS39110
FGA21,26
Penta D12,13,15
Penta E5,21
TH019.3
TPOX8 (Hysigen=TCH-C391)
8,9 (Millipore=SCC064; PubMed=24116068; Technion Genomics Center)
vWA17

Run an STR similarity search on this cell line
Publications

PubMed=15591520; DOI=10.1136/gut.2004.042127; PMCID=PMC1774377
Lie-Ming Xu, Alex Y. Hui, Efsevia Albanis, Michael James Paul Arthur, Sheila M. O'Byrne, William S. Blaner, Piali Mukherjee, Scott Laurence Friedman, Francis J. Eng;
Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis.
Gut 54:142-151(2005)

PubMed=17760834; DOI=10.1111/j.1582-4934.2007.00060.x; PMCID=PMC3823251
Jens Herrmann, Axel M. Gressner, Ralf Weiskirchen;
Immortal hepatic stellate cell lines: useful tools to study hepatic stellate cell biology and function?
J. Cell. Mol. Med. 11:704-722(2007)

PubMed=19085953; DOI=10.1002/hep.22697; PMCID=PMC2891538
Jin-Sheng Guo, Johnny Loke, Feng Zheng, Feng Hong, Steven Yea, Masayuki Fukata, Mirko Tarocchi, Olivia Tuason Abar, Hong-Jin Huang, John J. Sninsky, Scott Laurence Friedman;
Functional linkage of cirrhosis-predictive single nucleotide polymorphisms of Toll-like receptor 4 to hepatic stellate cell responses.
Hepatology 49:960-968(2009)

PubMed=21930125; DOI=10.1016/j.yexmp.2011.09.002
Andrielle Castilho-Fernandes, Danilo Candido de Almeida, Aparecida Maria Fontes, Fernanda Ursoli Ferreira Melo, Virginia Picanco-Castro, Marcela Cristina Correa de Freitas, Maristela Delgado Orellana, Patricia Vianna Bonini Palma, Perry B. Hackett, Scott Laurence Friedman, Dimas Tadeu Covas;
Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells.
Exp. Mol. Pathol. 91:664-672(2011)

PubMed=22694338; DOI=10.1021/pr3000927; PMCID=PMC3411196
S. Tamir Rashid, Jonathan D. Humphries, Adam Byron, Ameet Dhar, Janet A. Askari, Julian N. Selley, David Knight, Robert D. Goldin, Mark Thursz, Martin James Humphries;
Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver.
J. Proteome Res. 11:4052-4064(2012)

PubMed=24116068; DOI=10.1371/journal.pone.0075692; PMCID=PMC3792989
Ralf Weiskirchen, Jorg Weimer, Steffen K. Meurer, Anja Kron, Barbara Seipel, Inga Vater, Norbert Arnold, Reiner Siebert, Lie-Ming Xu, Scott Laurence Friedman, Carsten Bergmann;
Genetic characteristics of the human hepatic stellate cell line LX-2.
PLoS ONE 8:e75692.1-e75692.9(2013)

PubMed=34884585; DOI=10.3390/ijms222312782; PMCID=PMC8657869
Maximilian Schinagl, Tamara Tomin, Jurgen Gindlhuber, Sophie Honeder, Raphael Pfleger, Matthias Schittmayer, Michael Trauner, Ruth Birner-Gruenberger;
Proteomic changes of activated hepatic stellate cells.
Int. J. Mol. Sci. 22:12782.1-12782.17(2021)

Cross-references
Cell line collections (Providers) CLS; 305039
Hysigen; TCH-C391
Millipore; SCC064
Cell line databases/resources Lonza; 616
SKY/M-FISH/CGH; 5853
Anatomy/cell type resources BTO; BTO_0003516
Chemistry resources ChEMBL-Cells; CHEMBL4879539
Encyclopedic resources Wikidata; Q54903298
Experimental variables resources EFO; EFO_0022711
Proteomic databases PRIDE; PRD000680
PRIDE; PXD029121
Entry history
Entry creation04-Apr-2012
Last entry update31-Mar-2026
Version number29