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Cellosaurus LX-2 (CVCL_5792)

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Cell line name LX-2
Synonyms Lieming Xu-2
Accession CVCL_5792
Resource Identification Initiative To cite this cell line use: LX-2 (RRID:CVCL_5792)
Comments Characteristics: Able to grow under reduced serum conditions (1% FBS) (PubMed=15591520).
Doubling time: ~3-4 days (PubMed=15591520); 53.3 +- 2.3 hours (PubMed=21930125).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRSV-T).
Omics: Proteomics; Quantitative.
Omics: Proteomics; Subcellular; Extracellular matrix.
Anecdotal: Cell line name is derived from the name of the first author of the original paper.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatic stellate cell; CL=CL_0000632.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_5790 (LX-1 [Human HSC])
Children:
CVCL_E1LU (HyCyte LX-2 KO-hNGLY1)CVCL_F1RF (HyCyte LX-2 KO-hTPP1)CVCL_E3HV (HyCyte LX-2-Luc)
Sex of cell Male
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): Millipore=SCC064; PubMed=24116068; Technion Genomics Center

Markers:
AmelogeninX,Y
CSF1PO10,12
D1S165612
D2S44111,14
D2S133817
D3S135813,15
D5S81811,12
D7S82011
D8S117913
D10S124813
D12S39115,17
D13S31711,13
D16S53913
D18S5112
D19S43313,15.2
D21S1128,31
D22S104516
DYS39110
FGA21,26
Penta D12,13,15
Penta E5,21
TH019.3
TPOX8,9
vWA17

Run an STR similarity search on this cell line
Publications

PubMed=15591520; DOI=10.1136/gut.2004.042127; PMCID=PMC1774377
Lie-Ming Xu, Alex Y. Hui, Efsevia Albanis, Michael James Paul Arthur, Sheila M. O'Byrne, William S. Blaner, Piali Mukherjee, Scott Laurence Friedman, Francis J. Eng;
Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis.
Gut 54:142-151(2005)

PubMed=17760834; DOI=10.1111/j.1582-4934.2007.00060.x; PMCID=PMC3823251
Jens Herrmann, Axel M. Gressner, Ralf Weiskirchen;
Immortal hepatic stellate cell lines: useful tools to study hepatic stellate cell biology and function?
J. Cell. Mol. Med. 11:704-722(2007)

PubMed=19085953; DOI=10.1002/hep.22697; PMCID=PMC2891538
Jin-Sheng Guo, Johnny Loke, Feng Zheng, Feng Hong, Steven Yea, Masayuki Fukata, Mirko Tarocchi, Olivia Tuason Abar, Hong-Jin Huang, John J. Sninsky, Scott Laurence Friedman;
Functional linkage of cirrhosis-predictive single nucleotide polymorphisms of Toll-like receptor 4 to hepatic stellate cell responses.
Hepatology 49:960-968(2009)

PubMed=21930125; DOI=10.1016/j.yexmp.2011.09.002
Andrielle Castilho-Fernandes, Danilo Candido de Almeida, Aparecida Maria Fontes, Fernanda Ursoli Ferreira Melo, Virginia Picanco-Castro, Marcela Cristina Correa de Freitas, Maristela Delgado Orellana, Patricia Vianna Bonini Palma, Perry B. Hackett, Scott Laurence Friedman, Dimas Tadeu Covas;
Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells.
Exp. Mol. Pathol. 91:664-672(2011)

PubMed=22694338; DOI=10.1021/pr3000927; PMCID=PMC3411196
S. Tamir Rashid, Jonathan D. Humphries, Adam Byron, Ameet Dhar, Janet A. Askari, Julian N. Selley, David Knight, Robert D. Goldin, Mark Thursz, Martin James Humphries;
Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver.
J. Proteome Res. 11:4052-4064(2012)

PubMed=24116068; DOI=10.1371/journal.pone.0075692; PMCID=PMC3792989
Ralf Weiskirchen, Jorg Weimer, Steffen K. Meurer, Anja Kron, Barbara Seipel, Inga Vater, Norbert Arnold, Reiner Siebert, Lie-Ming Xu, Scott Laurence Friedman, Carsten Bergmann;
Genetic characteristics of the human hepatic stellate cell line LX-2.
PLoS ONE 8:e75692.1-e75692.9(2013)

PubMed=34884585; DOI=10.3390/ijms222312782; PMCID=PMC8657869
Maximilian Schinagl, Tamara Tomin, Jurgen Gindlhuber, Sophie Honeder, Raphael Pfleger, Matthias Schittmayer, Michael Trauner, Ruth Birner-Gruenberger;
Proteomic changes of activated hepatic stellate cells.
Int. J. Mol. Sci. 22:12782.1-12782.17(2021)

Cross-references
Cell line collections (Providers) CLS; 305039
Millipore; SCC064
Cell line databases/resources Lonza; 616
SKY/M-FISH/CGH; 5853
Anatomy/cell type resources BTO; BTO_0003516
Chemistry resources ChEMBL-Cells; CHEMBL4879539
Encyclopedic resources Wikidata; Q54903298
Experimental variables resources EFO; EFO_0022711
Proteomic databases PRIDE; PRD000680
PRIDE; PXD029121
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number28