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Cellosaurus Bhas42 (CVCL_5494)

[Text version]
Cell line name Bhas42
Synonyms Bhas 42
Accession CVCL_5494
Resource Identification Initiative To cite this cell line use: Bhas42 (RRID:CVCL_5494)
Comments Problematic cell line: Misidentified/contaminated. Not from strain BALB/c as originally assigned but from a NIH Swiss strain derivative (PubMed=27844419).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00530.
Biotechnology: Part of the 'Bhas 42 cell transformation assay' which is a sensitive short-term system for predicting chemical carcinogenicity (PubMed=21801851).
Transfected with: UniProtKB; P01115; Harvey murine sarcoma virus H-Ras (V-Ha-ras).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: v-Ha-Ras.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: Swiss albino.
Hierarchy Parent: CVCL_J646 (BALB/3T3 A31-1-1)
Category Transformed cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx

PubMed=3141328; DOI=10.1111/j.1349-7006.1988.tb00056.x
Sasaki K., Mizusawa H., Ishidate M.
Isolation and characterization of ras-transfected BALB/3T3 clone showing morphological transformation by 12-O-tetradecanoyl-phorbol-13-acetate.
Jpn. J. Cancer Res. 79:921-930(1988)

PubMed=21801851; DOI=10.1016/j.mrgentox.2011.07.006
Sakai A., Sasaki K., Hayashi K., Muramatsu D., Arai S., Endou N., Kuroda S., Poth A., Bohnenberger S., Kunkelmann T., Asakura M., Hirose H., Ishii N., Mizuhashi F., Kasamoto S., Nagai M., Pant K., Bruce S.W., Sly J.E., Yamazaki S., Umeda M., Tanaka N.
An international validation study of a Bhas 42 cell transformation assay for the prediction of chemical carcinogenicity.
Mutat. Res. 725:57-77(2011)

PubMed=27844419; DOI=10.1007/s11626-016-0104-3
Uchio-Yamada K., Kasai F., Ozawa M., Kohara A.
Incorrect strain information for mouse cell lines: sequential influence of misidentification on sublines.
In Vitro Cell. Dev. Biol. Anim. 53:225-230(2017)

Cell line collections (Providers) JCRB; JCRB0149
Encyclopedic resources Wikidata; Q54796337
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number13