ID   Bhas42
AC   CVCL_5494
SY   Bhas 42
DR   JCRB; JCRB0149
DR   Wikidata; Q54796337
RX   PubMed=3141328;
RX   PubMed=21801851;
RX   PubMed=27844419;
WW   Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
CC   Problematic cell line: Misidentified/contaminated. Not from strain BALB/c as originally assigned but from a NIH Swiss strain derivative (PubMed=27844419).
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00530.
CC   Biotechnology: Part of the 'Bhas 42 cell transformation assay' which is a sensitive short-term system for predicting chemical carcinogenicity (PubMed=21801851).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P01115; Harvey murine sarcoma virus H-Ras (V-Ha-ras).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Transformant: v-Ha-Ras.
CC   Breed/subspecies: Swiss albino.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_J646 ! BALB/3T3 A31-1-1
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 14-08-25; Version: 16
//
RX   PubMed=3141328; DOI=10.1111/j.1349-7006.1988.tb00056.x; PMCID=PMC5917616;
RA   Sasaki, Kiyoshi
RA   Mizusawa, Hiroshi
RA   Ishidate, Motoi
RT   "Isolation and characterization of ras-transfected BALB/3T3 clone
RT   showing morphological transformation by
RT   12-O-tetradecanoyl-phorbol-13-acetate.";
RL   Jpn. J. Cancer Res. 79:921-930(1988).
//
RX   PubMed=21801851; DOI=10.1016/j.mrgentox.2011.07.006;
RA   Sakai, Ayako
RA   Sasaki, Kiyoshi
RA   Hayashi, Kumiko
RA   Muramatsu, Dai
RA   Arai, Shoko
RA   Endou, Nobuko
RA   Kuroda, Sachiko
RA   Poth, Albrecht
RA   Bohnenberger, Susanne
RA   Kunkelmann, Thorsten
RA   Asakura, Masumi
RA   Hirose, Hideki
RA   Ishii, Nana
RA   Mizuhashi, Fukutaro
RA   Kasamoto, Sawako
RA   Nagai, Miho
RA   Pant, Kamala
RA   Bruce, Shannon W.
RA   Sly, Jamie E.
RA   Yamazaki, Shojiro
RA   Umeda, Makoto
RA   Tanaka, Noriho
RT   "An international validation study of a Bhas 42 cell transformation
RT   assay for the prediction of chemical carcinogenicity.";
RL   Mutat. Res. 725:57-77(2011).
//
RX   PubMed=27844419; DOI=10.1007/s11626-016-0104-3; PMCID=PMC5348555;
RA   Uchio-Yamada, Kozue
RA   Kasai, Fumio
RA   Ozawa, Midori
RA   Kohara, Arihiro
RT   "Incorrect strain information for mouse cell lines: sequential
RT   influence of misidentification on sublines.";
RL   In Vitro Cell. Dev. Biol. Anim. 53:225-230(2017).
//