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Cellosaurus MGC-803 (CVCL_5334)

[Text version]
Cell line name MGC-803
Synonyms MGC803; MGc80-3
Accession CVCL_5334
Resource Identification Initiative To cite this cell line use: MGC-803 (RRID:CVCL_5334)
Comments Problematic cell line: Contaminated. Seems to be an hybrid of HeLa and probably of a gastric cancer cell from an Asian individual. The STR profile is highly similar to that of HeLa yet shows some specific differences that suggest it is an hybrid cell line (PubMed=37543987; PubMed=38079103). Was originally thought to originate from a 53 year old male patient suffering from a poorly differentiated gastric mucinous adenocarcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00588.
Doubling time: ~25 hours (CelloPub=CLPUB00751); 22.5 hours (PubMed=29683800).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Proteomics; Subcellular; Secretome; 2DE-MS.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Miscellaneous: Formerly the CCRID database had 2 entries describing this cell line (3111C0001CCC000227, 3131C0001000700084). They were one of the sources for the STR profile of this entry.
Misspelling: BGC-803; Note=Occasionally.
Misspelling: BGC803; Note=Occasionally.
Misspelling: MCG803; GEO=GSM844588.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Children:
CVCL_UI41 (MGC-803/5FU)CVCL_UI42 (MGC-803/DDP)CVCL_VG42 (MGC-803/PTX)
CVCL_UI43 (MGC-803/TA)CVCL_E0Z6 (Ubigene MGC-803 DSC2 KO)
Category Hybrid cell line
STR profile Source(s): CCRID; PubMed=28107433; PubMed=28851942; PubMed=37543987; PubMed=38079103

Markers:
AmelogeninX
CSF1PO9,10,12
D1S165612,15,16
D2S44110,11
D2S133817,24
D3S135815,18
D5S81810,11,12
D6S104318,19
D7S82011,12
D8S117912,14
D10S124815
D12S39117,20,26
D13S3177,13.3
D16S5399,11
D18S5113
D19S43313
D21S1127,28
D22S104515,17
FGA18,21
Penta D8,15
Penta E7,10,16,17
TH017,9
TPOX12
vWA16,17,18

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

CLPUB00751
Wang K.-H.
A in vitro cell (MGc80-3) of a poorly differentiated mucoid adenocarcinoma of human stomach.
Shi Yan Sheng Wu Xue Bao 16:257-266(1983)

DOI=10.1007/BF02948354
Xiao H., Dong Z.-W., Shou C.-C.
Induction of apoptosis in human gastric carcinoma cell line MGC-803 by monoclonal antibody PD4.
Chin. J. Cancer Res. 10:169-173(1998)

PubMed=24743803
Huang H., Han Y., Wu J., Tian Z.-H., Qu L.-K., Shou C.-C.
Establishment of drug resistant cell line of MGC-803 and analysis of differential secretome.
Beijing Da Xue Xue Bao Yi Xue Ban 46:183-189(2014)

PubMed=28107433; DOI=10.1371/journal.pone.0170384; PMCID=PMC5249119
Huang Y.-Q., Liu Y.-H., Zheng C.-Y., Shen C.
Investigation of cross-contamination and misidentification of 278 widely used tumor cell lines.
PLoS ONE 12:e0170384.1-e0170384.16(2017)

PubMed=28851942; DOI=10.1038/s41598-017-09660-w; PMCID=PMC5575032
Bian X.-C., Yang Z.-L., Feng H.-L., Sun H., Liu Y.-Q.
A combination of species identification and STR profiling identifies cross-contaminated cells from 482 human tumor cell lines.
Sci. Rep. 7:9774-9774(2017)

PubMed=29683800; DOI=10.1097/CAD.0000000000000601
Fu L., Yin F., Li X.-R., Han B.-K., Zhang C., Wang J.-W., Wang Y.-Q., Bi Y.-F., Liu H.-M.
Generation and characterization of a paclitaxel-resistant human gastric carcinoma cell line.
Anticancer Drugs 29:491-502(2018)

PubMed=29717028; DOI=10.1042/BSR20180277; PMCID=PMC6050193
Zhang Y.-Q., Yu S.-T., Zhang Z.-Z., Zhao G., Xu J.
Long non-coding RNA AK096174 promotes cell proliferation and invasion in gastric cancer by regulating WDR66 expression.
Biosci. Rep. 38:BSR20180277.1-BSR20180277.12(2018)

PubMed=37543987; DOI=10.1002/ijc.34677
Cao F., Sun H., Yang Z.-L., Bai Y.-H., Hu X., Hou Y.-H., Bian X.-C., Liu Y.-Q.
Multiple approaches revealed MGc80-3 as a somatic hybrid with HeLa cells rather than a gastric cancer cell line.
Int. J. Cancer 154:155-168(2024)

PubMed=38079103; DOI=10.1007/s13577-023-01011-4
Yang M.-M., He J., Xia S.-X., Wang Y.-D., Xiong J., Liao C., Li N., Qu S.-F., Shen C.
Investigation of the mixed origins of the MGC-803 cell line reveals that it is a hybrid cell line derived from HeLa.
Hum. Cell 37:560-566(2024)

Cross-references
Cell line collections (Providers) Ubigene; YC-C081
Anatomy/cell type resources BTO; BTO_0004953
Chemistry resources ChEMBL-Cells; CHEMBL3706567
ChEMBL-Targets; CHEMBL3706566
PubChem_Cell_line; CVCL_5334
Encyclopedic resources Wikidata; Q54905425
Experimental variables resources EFO; EFO_0006286
Gene expression databases GEO; GSM844588
GEO; GSM1374671
Polymorphism and mutation databases Cosmic; 2267321
IARC_TP53; 24858
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number31