| Abstract |
This work deals with a new cell line (MGc80-3) established from a primary
poorly differentiated mucoid adenocarcinoma of human stomach. The tumor
cells were derived from the gastric cancer of a patient on March 10,1980,
and a new permanent cell line was established after a latent period of 9
months. The cells grew in a monolayered sheet with a population doubling
time of about 25 hrs. Under phase contrast microscope, the cells were
characterized morphologically as single nucleated epitheloid cells with
prominent nucleoli and rather scanty cytoplasm. The nucleoli were very
distinct and irregular in shape. All features from our transmission and
scanning electron microscopical observations showed that the cells of
MGc-80-3 cell line have the characteristics of malignant mucoid gland cells
with vigorous metabolism. The features are: high nuclear/cytoplasmic ratio;
many prominent evaginations of wrinkled cytoplasmic membrane forming
finger-like microvilli; rich cytoplasmic organelles and abundant
polyribosome at secretory stage with plenty of mucoid granules located at
one end of the cells. This tumor cell line can be transplanted and grown
subcutaneously into tumor nodules in cortisone treated irradiated rats.
The chromosome make-up of MGc80-3 is hypertriploid with a model number of
75.
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