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Cellosaurus AC16 [Human hybrid cardiomyocyte] (CVCL_4U18)

[Text version]
Cell line name AC16 [Human hybrid cardiomyocyte]
Accession CVCL_4U18
Resource Identification Initiative To cite this cell line use: AC16 [Human hybrid cardiomyocyte] (RRID:CVCL_4U18)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-1500.
Characteristics: Hybrid of adult ventricular cardiomiocytes with a SV40 transformed, uridine auxotroph human fibroblast cell line devoid of mitochondrial DNA (PubMed=15913645).
Characteristics: Can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes (Millipore=SCC109).
Doubling time: 24.49 hours (PubMed=15913645).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRNS-1).
Derived from site: In situ; Heart, ventricle; UBERON=UBERON_0002082.
Cell type: Cardiomyocyte; CL=CL_0002495.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_4U14 (DWFb1-rho0)
Children:
CVCL_E2Q0 (HyCyte AC16 hRYR2_p.D179E_c.537T>G)CVCL_E1J3 (HyCyte AC16 KO-hCGAS)CVCL_D8DN (Ubigene AC16 RBM15 KO)
Category Hybrid cell line
STR profile Source(s): Millipore=SCC109

Markers:
AmelogeninX
CSF1PO9,11,12
D3S135817,18
D5S8189,11
D7S82010,11,12
D8S117912,14
D13S31712,13
D16S53911,13
D18S5112,17
D21S1132.2,33.2
FGA21,25
Penta D2.2,9
Penta E7,8,16
TH017,8,9.3
TPOX11
vWA16,18

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Publications

PubMed=15913645; DOI=10.1016/j.yjmcc.2005.03.003
Mercy Mascren Davidson, Claudia Nesti, Lluis Palenzuela, Winsome F. Walker, Evelyn Hernandez, Lev Protas, Michio Hirano, Nithila Doraipandyan Isaac;
Novel cell lines derived from adult human ventricular cardiomyocytes.
J. Mol. Cell. Cardiol. 39:133-147(2005)

Patent=US7223599
Mercy Mascren Davidson;
Immortalization of human post-mitotic cells.
Patent number US7223599, 29-May-2007

Cross-references
Cell line collections (Providers) ATCC; CRL-3568
CLS; 305215
Millipore; SCC109
Encyclopedic resources Wikidata; Q54607923
Experimental variables resources EFO; EFO_0022630
Entry history
Entry creation22-Sep-2015
Last entry update14-Aug-2025
Version number11