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Cellosaurus HuH-6 (CVCL_4381)

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Cell line name HuH-6
Synonyms HUH-6; HuH 6; HuH6; HUH6; Huh6
Accession CVCL_4381
Resource Identification Initiative To cite this cell line use: HuH-6 (RRID:CVCL_4381)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: JFCR45 cancer cell line panel.
Population: Japanese.
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Gly34Val (c.101G>T); ClinVar=VCV000017582; Zygosity=Heterozygous (PubMed=9671767; DepMap=ACH-000671).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Ala159Asp (c.476C>A); ClinVar=VCV001026335; Zygosity=Heterozygous (DepMap=ACH-000671).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Asn239Asp (c.715A>G); ClinVar=VCV000234036; Zygosity=Heterozygous (DepMap=ACH-000671).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.79
Native American0.3
East Asian, North80.92
East Asian, South17.99
South Asian0
European, North0
European, South0
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_1296 (HuH-6 Clone 5)CVCL_JF84 (HuH-6-Luc)CVCL_M176 (HUH6-eGFP)
Sex of cell Male
Age at sampling 1Y
Category Cancer cell line
STR profile Source(s): CCRID; RCB=RCB1367

Markers:
AmelogeninX,Y
CSF1PO10,12
D2S133818
D3S135814,17
D5S8188,11
D7S82011,12
D8S117910,11
D13S3178,12
D16S53910,11
D18S5113,21
D19S43312,12.2
D21S1129,30
FGA19,24
TH017,8
TPOX8
vWA14,17

Run an STR similarity search on this cell line
Web pages Info; LCCL; -; https://lccl.zucmanlab.com/hcc/cellLines/Huh6
Publications

PubMed=57894
Isao Doi;
Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma.
Gann 67:1-10(1976)

DOI=10.11418/jtca1981.12.3_221
Masayoshi Namba, Hidekazu Nakabayashi, Isao Doi, Jiro Sato, Masahiro Miyazaki;
Cellular characteristics and utilization of human hepatoma cell lines which were established in our laboratory and distributed by Japanese Cancer Research Resources Bank.
Tissue Cult. Res. Commun. 12:221-227(1993)

PubMed=8389256; DOI=10.1093/carcin/14.5.987
Ih-Chang Hsu, Takayoshi Tokiwa, William P. Bennett, Robert A. Metcalf, Judith A. Welsh, Tsung-Tang Sun, Curtis Craig Harris;
p53 gene mutation and integrated hepatitis B viral DNA sequences in human liver cancer cell lines.
Carcinogenesis 14:987-992(1993)

DOI=10.11418/jtca1981.16.3_173
Koichiro Mihara, Masahiro Miyazaki, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Ryuichiro Ohashi, Masayoshi Namba;
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9359923; DOI=10.18926/AMO/30789
Koichiro Mihara, Masahiro Miyazaki, Tadashi Kondo, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Chikashi Ishioka, Masayoshi Namba;
Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.
Acta Med. Okayama 51:261-265(1997)

PubMed=9671767; DOI=10.1073/pnas.95.15.8847; PMCID=PMC21165
Alix de la Coste, Beatrice Romagnolo, Pierre Billuart, Claire-Angelique Renard, Marie-Annick Buendia, Olivier Soubrane, Monique Fabre, Jamel Chelly, Cherif Beldjord, Axel Kahn, Christine Perret;
Somatic mutations of the beta-catenin gene are frequent in mouse and human hepatocellular carcinomas.
Proc. Natl. Acad. Sci. U.S.A. 95:8847-8851(1998)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Noriyuki Nakatsu, Yoko Yoshida, Kanami Yamazaki, Tomoki Nakamura, Shingo Dan, Yasuhisa Fukui, Takao Yamori;
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=20937217; DOI=10.1170/149
Alessandra Di Masi, Mara Viganotti, Antonio Antoccia, Armando Magrelli, Marco Salvatore, Gianluca Azzalin, Fabrizio Tosto, Stefano Lorenzetti, Francesca Maranghi, Alberto Mantovani, Giuseppe Macino ...Show all 13 authors... , Caterina Tanzarella, Domenica Taruscio; Show fewer authors
Characterization of HuH6, Hep3B, HepG2 and HLE liver cancer cell lines by WNT/beta-catenin pathway, microRNA expression and protein expression profile.
Cell. Mol. Biol. 56:OL1299-OL1317(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23505090; DOI=10.1002/hep.26402
Kai Wang, Ho Yeong Lim, Stephanie Shi, Jeeyun Lee, Shi-Bing Deng, Tao Xie, Zhou Zhu, Yu-Li Wang, David Pocalyko, Wei Jennifer Yang, Paul A. Rejto ...Show all 14 authors... , Mao Mao, Cheol-Keun Park, Jiang-Chun Xu; Show fewer authors
Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.
Hepatology 58:706-717(2013)

PubMed=23887712; DOI=10.1038/ncomms3218; PMCID=PMC3731665
Jean-Charles Nault, Maxime Mallet, Camilla Pilati, Julien Calderaro, Paulette Bioulac-Sage, Christophe Laurent, Alexis Laurent, Daniel Cherqui, Charles Balabaud, Jessica Zucman-Rossi;
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Nat. Commun. 4:2218.1-2218.7(2013)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31063779; DOI=10.1053/j.gastro.2019.05.001
Stefano Caruso, Anna-Line Calatayud, Jill Pilet, Tiziana La Bella, Samia Rekik, Sandrine Imbeaud, Eric Letouze, Lea Meunier, Quentin Bayard, Nataliya Rohr-Udilova, Camille Peneau ...Show all 20 authors... , Bettina Grasl-Kraupp, Leanne de Koning, Berengere Ouine, Paulette Bioulac-Sage, Gabrielle Couchy, Julien Calderaro, Jean-Charles Nault, Jessica Zucman-Rossi, Sandra Rebouissou; Show fewer authors
Analysis of liver cancer cell lines identifies agents with likely efficacy against hepatocellular carcinoma and markers of response.
Gastroenterology 157:760-776(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=32899426; DOI=10.3390/cancers12092510; PMCID=PMC7565451
Dominique Scherer, Marcela Davila-Lopez, Benjamin Goeppert, Sanna Abrahamsson, Rosa Gonzalez Silos, Igor Nova, Katherine Marcelain, Juan Carlos Roa, David Ibberson, Sinan U. Umu, Trine Ballestad Rounge ...Show all 13 authors... , Stephanie Roessler, Justo Lorenzo-Bermejo; Show fewer authors
RNA sequencing of hepatobiliary cancer cell lines: data and applications to mutational and transcriptomic profiling.
Cancers (Basel) 12:2510.1-2510.14(2020)

Cross-references
Cell line collections (Providers) ABM; T9914
CLS; 305092
RCB; RCB1367
Cell line databases/resources CLO; CLO_0050851
cancercelllines; CVCL_4381
CCRID; 1101HUM-PUMC000477
CCRID; 3101HUMTCHu181
Cell_Model_Passport; SIDM00062
DepMap; ACH-000671
Anatomy/cell type resources BTO; BTO_0001670
Biological sample resources BioSample; SAMN10987713
CRISP screens repositories BioGRID_ORCS_Cell_line; 643
Encyclopedic resources Wikidata; Q54896848
Gene expression databases ArrayExpress; E-MTAB-2770
FANTOM5_SSTAR; 10633-108I3
GEO; GSM887148
GEO; GSM888220
GEO; GSM936762
Polymorphism and mutation databases Cosmic; 869168
Cosmic; 869279
Cosmic; 873714
Cosmic; 1187329
Cosmic; 1237546
Cosmic; 1518228
Cosmic; 1622750
Cosmic; 2162537
Cosmic; 2369478
Cosmic; 2771602
IARC_TP53; 28317
LiGeA; CCLE_149
Progenetix; CVCL_4381
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number38