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Cellosaurus HBL-100 (CVCL_4362)

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Cell line name HBL-100
Synonyms HBL 100; HBL100
Accession CVCL_4362
Resource Identification Initiative To cite this cell line use: HBL-100 (RRID:CVCL_4362)
Comments Problematic cell line: Misidentified. Presence of a Y chromosome in cell line that was thought to be of female origin (PubMed=16643607; PubMed=20143388). Originally thought to originate from a casein-producing breast cell line from a 27 year old Caucasian woman. In addition contains SV40 genomic sequence while the cell line was deemed to be spontaneously immortalized.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: GrayJW breast cancer cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00207.
Virology: Contains 2 copies of the SV40 genome, one complete the other incomplete tandemly integrated in the cell line genome.
Doubling time: 28 hours (Note=At 10th passage) (PubMed=6891286); 1.5 days (PubMed=9671407); ~40 hours (CLS=300178); 30.21 hours (GrayJW panel).
Karyotypic information: Near triploid with the modal number of 67 chromosomes (ATCC=HTB-124).
Microsatellite instability: Stable (MSS) (PubMed=12661003).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Omics: Array-based CGH.
Omics: Deep exome analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
HLA typing Source: PubMed=26589293
Class I
Class II
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
Sex of cell Male
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): ATCC; CCRID; CLS; PubMed=11416159; PubMed=25877200; PubMed=28889351

AmelogeninX (CCRID; PubMed=25877200; PubMed=28889351)
X,Y (ATCC; CLS; PubMed=11416159)
Penta D12
Penta E7

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-297.html

Polanowski F.P., Gaffney E.V., Burke R.E.
HBL-100, a cell line established from human breast milk.
In Vitro 12:328-328(1976)

PubMed=6891286; DOI=10.1007/BF00204786
Gaffney E.V.
A cell line (HBL-100) established from human breast milk.
Cell Tissue Res. 227:563-568(1982)

PubMed=6500159; DOI=10.1159/000163283
Gershwin M.E., Lentz D., Owens R.B.
Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.
Exp. Cell Biol. 52:361-370(1984)

PubMed=2995096; DOI=10.1016/0014-4827(85)90238-1
Caron de Fromentel C., Nardeux P.-C., Soussi T., Lavialle C., Estrade S., Carloni G., Chandrasekaran K., Cassingena R.
Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information.
Exp. Cell Res. 160:83-94(1985)

PubMed=3109929; DOI=10.1016/0014-4827(87)90318-1
Dealtry G.B., Naylor M.S., Fiers W.C., Balkwill F.R.
The effect of recombinant human tumour necrosis factor on growth and macromolecular synthesis of human epithelial cells.
Exp. Cell Res. 170:428-438(1987)

Marlhens F., Saint-Ruf C., Nardeux P.-C., Lavialle C., Brouty-Boye D., Dutrillaux B., Cassingena R.
Karyotype evolution of the human HBL-100 cell line and mapping of the integration site of SV40 DNA.
Ann. Genet. 31:81-86(1988)

PubMed=2547730; DOI=10.1002/ijc.2910440230
Saint-Ruf C., Nardeux P.-C., Cebrian J., Lacasa M., Lavialle C., Cassingena R.
Molecular cloning and characterization of endogenous SV40 DNA from human HBL-100 cells.
Int. J. Cancer 44:367-372(1989)

Leibovitz A.
Cell lines from human breast.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York (1994)

PubMed=9671407; DOI=10.1038/sj.onc.1201814
Sweeney K.J., Swarbrick A., Sutherland R.L., Musgrove E.A.
Lack of relationship between CDK activity and G1 cyclin expression in breast cancer cells.
Oncogene 16:2865-2878(1998)

Forozan F., Mahlamaki E.H., Monni O., Chen Y.-D., Veldman R., Jiang Y., Gooden G.C., Ethier S.P., Kallioniemi A., Kallioniemi O.-P.
Comparative genomic hybridization analysis of 38 breast cancer cell lines: a basis for interpreting complementary DNA microarray data.
Cancer Res. 60:4519-4525(2000)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=16643607; DOI=10.1111/j.1749-0774.2005.00007.x
Yoshino K., Iimura E., Saijo K., Iwase S., Fukami K., Ohno T., Obata Y., Nakamura Y.
Essential role for gene profiling analysis in the authentication of human cell lines.
Hum. Cell 19:43-48(2006)

PubMed=17157791; DOI=10.1016/j.ccr.2006.10.008
Neve R.M., Chin K., Fridlyand J., Yeh J., Baehner F.L., Fevr T., Clark L., Bayani N., Coppe J.-P., Tong F., Speed T., Spellman P.T., DeVries S., Lapuk A., Wang N.J., Kuo W.-L., Stilwell J.L., Pinkel D., Albertson D.G., Waldman F.M., McCormick F., Dickson R.B., Johnson M.D., Lippman M.E., Ethier S.P., Gazdar A.F., Gray J.W.
A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes.
Cancer Cell 10:515-527(2006)

Morrison B.J.
Breast cancer stem cells: tumourspheres and implications for therapy.
Thesis PhD (2010), Griffith University, Australia

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=24094812; DOI=10.1016/j.ccr.2013.08.020
Timmerman L.A., Holton T., Yuneva M., Louie R.J., Padro M., Daemen A., Hu M., Chan D.A., Ethier S.P., van 't Veer L.J., Polyak K., McCormick F., Gray J.W.
Glutamine sensitivity analysis identifies the xCT antiporter as a common triple-negative breast tumor therapeutic target.
Cancer Cell 24:450-465(2013)

PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110
Daemen A., Griffith O.L., Heiser L.M., Wang N.J., Enache O.M., Sanborn Z., Pepin F., Durinck S., Korkola J.E., Griffith M., Hur J.S., Huh N., Chung J., Cope L., Fackler M.J., Umbricht C.B., Sukumar S., Seth P., Sukhatme V.P., Jakkula L.R., Lu Y.-L., Mills G.B., Cho R.J., Collisson E.A., van 't Veer L.J., Spellman P.T., Gray J.W.
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=28889351; DOI=10.1007/s10549-017-4496-x
Saunus J.M., Smart C.E., Kutasovic J.R., Johnston R.L., Kalita-de Croft P., Miranda M., Rozali E.N., Vargas A.C., Reid L.E., Lorsy E., Cocciardi S., Seidens T., McCart Reed A.E., Dalley A.J., Wockner L.F., Johnson J., Sarkar D., Askarian-Amiri M.E., Simpson P.T., Khanna K.K., Chenevix-Trench G., Al-Ejeh F., Lakhani S.R.
Multidimensional phenotyping of breast cancer cell lines to guide preclinical research.
Breast Cancer Res. Treat. 167:289-301(2018)

Cell line collections (Providers) ATCC; HTB-124 - Discontinued
CLS; 300178
RCB; RCB0460 - Discontinued
Ubigene; YC-C013
Cell line databases/resources CLO; CLO_0003626
CLDB; cl1571
CCRID; 1101HUM-PUMC000374
DepMap; ACH-002321
LINCS_HMS; 51117
TOKU-E; 3589
Anatomy/cell type resources BTO; BTO:0004734
Biological sample resources BioSample; SAMN03152011
BioSample; SAMN03471101
BioSample; SAMN03472914
Chemistry resources ChEMBL-Cells; CHEMBL3307713
ChEMBL-Targets; CHEMBL614811
PharmacoDB; HBL100_443_2019
PubChem_Cell_line; CVCL_4362
Encyclopedic resources Wikidata; Q54881428
Experimental variables resources EFO; EFO_0001167
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-TABM-157
GEO; GSM1053697
Polymorphism and mutation databases Cosmic; 687471
Cosmic; 877447
Cosmic; 934533
Cosmic; 979722
Cosmic; 991323
Cosmic; 1010933
Cosmic; 1136364
Cosmic; 1176632
Cosmic; 1434949
Cosmic; 1524348
Cosmic; 1609469
IARC_TP53; 21063
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number35