Cellosaurus logo
expasy logo

Cellosaurus Dami (CVCL_4360)

[Text][XML][JSON]
Cell line name Dami
Synonyms DAMI
Accession CVCL_4360
Resource Identification Initiative To cite this cell line use: Dami (RRID:CVCL_4360)
Comments Group: Patented cell line.
Problematic cell line: Contaminated. Shown to be a HEL derivative (PubMed=9166871; PubMed=9326259; PubMed=9447816; PubMed=10508494; PubMed=12592342; PubMed=20143388). Originally thought to originate from the peripheral blood of a 57 year old male patient with megakaryoblastic leukemia.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00116.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-9792.
Population: Caucasian.
Doubling time: 24-30 hours (PubMed=3196874; Patent=US5128245).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: Erythroblast; CL=CL_0000765.
Sequence variations
  • Mutation; HGNC; HGNC:6192; JAK2; Simple; p.Val617Phe (c.1849G>T); ClinVar=VCV000014662; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Met133Lys (c.398T>A); ClinVar=VCV002430159; Zygosity=Homozygous (from parent cell line).
Disease Erythroleukemia (NCIt: C7152)
Acute erythroid leukemia (ORDO: Orphanet_318)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0001 (HEL)
Sex of cell Male
Age at sampling 30Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-9792; CCRID

Markers:
AmelogeninX (CCRID)
X,Y (ATCC=CRL-9792)
CSF1PO10
D2S133818,19
D3S135815
D5S81811
D7S8207
D8S117913,15
D13S3179,11
D16S53911
D18S5112,16
D19S43311,13
D21S1129,31.2
FGA22,23
TH017
TPOX11
vWA14,17

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/dami-atcc-crl-9792
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=3196874; DOI=10.1182/blood.V72.6.1968.1968
Sheryl M. Greenberg, David S. Rosenthal, Tammy A. Greeley, Ramana Tantravahi, Robert I. Handin;
Characterization of a new megakaryocytic cell line: the Dami cell.
Blood 72:1968-1977(1988)

Patent=US5128245
Sheryl M. Greenberg, Robert I. Handin;
Establishment, characterization and differentiation of a new megakaryocytic cell line, the Dami cells.
Patent number US5128245, 07-Jul-1992

PubMed=9166871; DOI=10.1182/blood.V89.11.4238
Robert I. Handin;
DAMI cell line.
Blood 89:4238-4238(1997)

PubMed=9326259; DOI=10.1182/blood.V90.7.2850
Roderick A.F. MacLeod, Wilhelm Gerhard Dirks, Hans Gunther Drexler;
Early contamination of the Dami cell line by HEL.
Blood 90:2850-2851(1997)

PubMed=9447816; DOI=10.1038/sj.leu.2400868
Roderick A.F. MacLeod, Wilhelm Gerhard Dirks, Yvonne A. Reid, Robert J. Hay, Hans Gunther Drexler;
Identity of original and late passage Dami megakaryocytes with HEL erythroleukemia cells shown by combined cytogenetics and DNA fingerprinting.
Leukemia 11:2032-2038(1997)

PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2
Roderick A.F. MacLeod, Wilhelm Gerhard Dirks, Yoshinobu Matsuo, Maren Kaufmann, Herbert Milch, Hans Gunther Drexler;
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Int. J. Cancer 83:555-563(1999)

PubMed=11021758; DOI=10.1038/sj.leu.2401891
Marcin Majka, Tomasz Rozmyslowicz, Marek J. Honczarenko, Janina Ratajczak, Mariusz A. Wasik, Glen N. Gaulton, Mariusz Zdzislaw Ratajczak;
Biological significance of the expression of HIV-related chemokine coreceptors (CCR5 and CXCR4) and their ligands by human hematopoietic cell lines.
Leukemia 14:1821-1832(2000)

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Hans Gunther Drexler, Wilhelm Gerhard Dirks, Yoshinobu Matsuo, Roderick A.F. MacLeod;
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections (Providers) ATCC; CRL-9792 - Discontinued
KCB; KCB 200782YJ
Cell line databases/resources cancercelllines; CVCL_4360
Lonza; 1426
Anatomy/cell type resources BTO; BTO_0001550
Biological sample resources BioSample; SAMN03151657
Encyclopedic resources Wikidata; Q54828561
Polymorphism and mutation databases Cosmic; 1191696
Entry history
Entry creation04-Apr-2012
Last entry update27-Nov-2025
Version number34