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Cellosaurus LNZTA3WT4 (CVCL_3540)

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Cell line name LNZTA3WT4
Synonyms LNZTA3p53WT4; WT4
Accession CVCL_3540
Resource Identification Initiative To cite this cell line use: LNZTA3WT4 (RRID:CVCL_3540)
Comments Group: Patented cell line.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-11543.
Population: Caucasian.
Characteristics: The expression of the luciferase reporter gene as well as the TP53 gene are under the control of a tetO operator.
Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
Microsatellite instability: Stable (MSS) (Sanger).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:11998; TP53.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Brain, right parieto-occipital lobe.
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.42
Native American1.36
East Asian, North1.52
East Asian, South0
South Asian1.65
European, North44.35
European, South49.7
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0394 (LN-308)
Sex of cell Male
Age at sampling 60Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-11543; Cosmic-CLP=1240170

Markers:
AmelogeninX,Y
CSF1PO11,12
D5S81812
D7S82010,12
D13S31711
D16S5399,12
TH019.3
TPOX8,9
vWA15,17

Run an STR similarity search on this cell line
Publications

Patent=US5807731
Erwin G. Van Meir, H.-J. Su Huang, Webster K. Cavenee;
Glioblastoma cell lines that produce angiogenesis inhibiting factor.
Patent number US5807731, 15-Sep-1998

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-11543 - Discontinued
Cell line databases/resources CLO; CLO_0007371
cancercelllines; CVCL_3540
Cell_Model_Passport; SIDM01220
Cosmic-CLP; 1240170
DepMap; ACH-002269
LINCS_HMS; 50028
LINCS_LDP; LCL-1345
Biological sample resources BioSample; SAMN03471340
CRISP screens repositories BioGRID_ORCS_Cell_line; 931
Chemistry resources GDSC; 1240170
PharmacoDB; LNZTA3WT4_853_2019
Encyclopedic resources Wikidata; Q54902866
Gene expression databases ArrayExpress; E-MTAB-3610
GEO; GSM1670052
Polymorphism and mutation databases Cosmic; 1995487
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number33