ID   LNZTA3WT4
AC   CVCL_3540
SY   LNZTA3p53WT4; WT4
DR   CLO; CLO_0007371
DR   ArrayExpress; E-MTAB-3610
DR   ATCC; CRL-11543
DR   BioGRID_ORCS_Cell_line; 931
DR   BioSample; SAMN03471340
DR   cancercelllines; CVCL_3540
DR   Cell_Model_Passport; SIDM01220
DR   Cosmic; 1995487
DR   Cosmic-CLP; 1240170
DR   DepMap; ACH-002269
DR   EGA; EGAS00001000978
DR   GDSC; 1240170
DR   GEO; GSM1670052
DR   LINCS_HMS; 50028
DR   LINCS_LDP; LCL-1345
DR   PharmacoDB; LNZTA3WT4_853_2019
DR   PRIDE; PXD030304
DR   Wikidata; Q54902866
RX   Patent=US5807731;
RX   PubMed=27397505;
RX   PubMed=30894373;
RX   PubMed=30971826;
RX   PubMed=35839778;
CC   Group: Patented cell line.
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Part of: COSMIC cell lines project.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-11543.
CC   Population: Caucasian.
CC   Characteristics: The expression of the luciferase reporter gene as well as the TP53 gene are under the control of a tetO operator.
CC   Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
CC   Microsatellite instability: Stable (MSS) (Sanger).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:11998; TP53.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
CC   Omics: Genomics; DNA methylation analysis.
CC   Omics: Genomics; Whole exome sequencing.
CC   Omics: Phenotyping; CRISPR screening.
CC   Omics: Phenotyping; Drug screening.
CC   Omics: Proteomics; Quantitative.
CC   Omics: Transcriptomics; Microarray.
CC   Genome ancestry: African=1.42%; Native American=1.36%; East Asian, North=1.52%; East Asian, South=0%; South Asian=1.65%; European, North=44.35%; European, South=49.7% (PubMed=30894373).
CC   Discontinued: ATCC; CRL-11543; true.
CC   Derived from site: In situ; Brain, right parieto-occipital lobe.
ST   Source(s): ATCC=CRL-11543; Cosmic-CLP=1240170
ST   Amelogenin: X,Y
ST   CSF1PO: 11,12
ST   D13S317: 11
ST   D16S539: 9,12
ST   D5S818: 12
ST   D7S820: 10,12
ST   TH01: 9.3
ST   TPOX: 8,9
ST   vWA: 15,17
DI   NCIt; C60781; Astrocytoma
DI   ORDO; Orphanet_94; Astrocytoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0394 ! LN-308
SX   Male
AG   60Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 14-08-25; Version: 33
//
RX   PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775;
RA   Goncalves, Emanuel
RA   Poulos, Rebecca C.
RA   Cai, Zhao-Xiang
RA   Barthorpe, Syd
RA   Manda, Srikanth S.
RA   Lucas, Natasha
RA   Beck, Alexandra
RA   Bucio-Noble, Daniel
RA   Dausmann, Michael
RA   Hall, Caitlin
RA   Hecker, Michael
RA   Koh, Jennifer
RA   Lightfoot, Howard
RA   Mahboob, Sadia
RA   Mali, Iman
RA   Morris, James
RA   Richardson, Laura
RA   Seneviratne, Akila J.
RA   Shepherd, Rebecca
RA   Sykes, Erin
RA   Thomas, Frances
RA   Valentini, Sara
RA   Williams, Steven G.
RA   Wu, Yang-Xiu
RA   Xavier, Dylan
RA   MacKenzie, Karen L.
RA   Hains, Peter G.
RA   Tully, Brett
RA   Robinson, Phillip James
RA   Zhong, Qing
RA   Garnett, Mathew J.
RA   Reddel, Roger Robert
RT   "Pan-cancer proteomic map of 949 human cell lines.";
RL   Cancer Cell 40:835-849.e8(2022).
//
RX   PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469;
RA   Iorio, Francesco
RA   Knijnenburg, Theo A.
RA   Vis, Daniel J.
RA   Bignell, Graham Robert
RA   Menden, Michael Patrick
RA   Schubert, Michael
RA   Aben, Nanne
RA   Goncalves, Emanuel
RA   Barthorpe, Syd
RA   Lightfoot, Howard
RA   Cokelaer, Thomas
RA   Greninger, Patricia
RA   van Dyk, Ewald
RA   Chang, Han
RA   de Silva, Heshani
RA   Heyn, Holger
RA   Deng, Xian-Ming
RA   Egan, Regina K.
RA   Liu, Qing-Song
RA   Mironenko, Tatiana
RA   Mitropoulos, Xeni
RA   Richardson, Laura
RA   Wang, Jin-Hua
RA   Zhang, Ting-Hu
RA   Moran, Sebastian
RA   Sayols, Sergi
RA   Soleimani, Maryam
RA   Tamborero, David
RA   Lopez-Bigas, Nuria
RA   Ross-Macdonald, Petra
RA   Esteller, Manel
RA   Gray, Nathanael S.
RA   Haber, Daniel Arie
RA   Stratton, Michael Rudolf
RA   Benes, Cyril Henri
RA   Wessels, Lodewyk F.A.
RA   Saez-Rodriguez, Julio
RA   McDermott, Ultan
RA   Garnett, Mathew J.
RT   "A landscape of pharmacogenomic interactions in cancer.";
RL   Cell 166:740-754(2016).
//
RX   PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675;
RA   Dutil, Julie
RA   Chen, Zhi-Hua
RA   Monteiro, Alvaro N.A.
RA   Teer, Jamie K.
RA   Eschrich, Steven A.
RT   "An interactive resource to probe genetic diversity and estimated
RT   ancestry in cancer cell lines.";
RL   Cancer Res. 79:1263-1273(2019).
//
RX   PubMed=30971826; DOI=10.1038/s41586-019-1103-9;
RA   Behan, Fiona M.
RA   Iorio, Francesco
RA   Picco, Gabriele
RA   Goncalves, Emanuel
RA   Beaver, Charlotte M.
RA   Migliardi, Giorgia
RA   Santos, Rita
RA   Rao, Yanhua
RA   Sassi, Francesco
RA   Pinnelli, Marika
RA   Ansari, Rizwan
RA   Harper, Sarah
RA   Jackson, David Adam
RA   McRae, Rebecca
RA   Pooley, Rachel
RA   Wilkinson, Piers
RA   van der Meer, Dieudonne J.
RA   Dow, David
RA   Buser-Doepner, Carolyn A.
RA   Bertotti, Andrea
RA   Trusolino, Livio
RA   Stronach, Euan Alexander
RA   Saez-Rodriguez, Julio
RA   Yusa, Kosuke
RA   Garnett, Mathew J.
RT   "Prioritization of cancer therapeutic targets using CRISPR-Cas9
RT   screens.";
RL   Nature 568:511-516(2019).
//
RX   Patent=US5807731;
RA   Van Meir, Erwin G.
RA   Huang, H.-J. Su
RA   Cavenee, Webster K.
RT   "Glioblastoma cell lines that produce angiogenesis inhibiting
RT   factor.";
RL   Patent number US5807731, 15-Sep-1998.
//