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Cellosaurus SUM149PT (CVCL_3422)

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Cell line name SUM149PT
Synonyms SUM-149PT; SUM 149PT; SUM149-PT; SUM149; SUM-149; SUM 149; 149 PT; 149PT; BrCL12
Accession CVCL_3422
Resource Identification Initiative To cite this cell line use: SUM149PT (RRID:CVCL_3422)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: JWGray breast cancer cell line panel.
Part of: KuDOS 95 cell line panel.
Characteristics: Derived from a mouse xenograft of a transplanted primary human invasive infiltrating ductal carcinoma metastatic nodule.
Doubling time: 33.95 hours (JWGray panel).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; PTM; Glycoproteome.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; CNV analysis.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Breast; UBERON=UBERON_0000310.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (PubMed=19593635).
  • Mutation; HGNC; HGNC:1100; BRCA1; Simple; p.Pro724Leufs*12 (c.2169delT) (p.Asn723fs*13) (2288delT); Zygosity=Hemizygous (PubMed=16397213; PubMed=19593635; PubMed=22032724).
  • Mutation; HGNC; HGNC:3373; EP300; Simple; c.4025+26_4025+30 (c.5224+28del3); Zygosity=Heterozygous (PubMed=10700188).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Met237Ile (c.711G>A); ClinVar=VCV000142714; Zygosity=Unspecified (PubMed=16541312).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*68:01,68:01
HLA-BB*53:01,78:01
HLA-CC*06:02,16:01
Disease Breast inflammatory carcinoma (NCIt: C4001)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_DH44 (SUM149-Luc)CVCL_C9AM (SUM149R)
Sex of cell Female
Age at sampling 40Y
Category Cancer cell line
STR profile Source(s): CLS=300609; DepMap=ACH-001390; PubMed=25877200

Markers:
AmelogeninX
CSF1PO12
D2S133820
D3S135817
D5S81811
D6S104318
D7S82011
D8S117914,16
D12S39115,18
D13S31712
D16S53911
D18S5114,15
D19S43312,14
D21S1128,31.2
FGA29
Penta D8,9
Penta E11
TH019.3
TPOX9
vWA16,18

Run an STR similarity search on this cell line
Web pages Info; Synapse; JWGray panel; https://www.synapse.org/#!Synapse:syn2346643/wiki/62255
Provider; BioIVT/Asterand; -; https://bioivt.com/sum-breast-cancer-cell-lines/
Publications

PubMed=10424408; DOI=10.1023/A:1006135331912
Kathleen M. Woods Ignatoski, Stephen P. Ethier;
Constitutive activation of pp125fak in newly isolated human breast cancer cell lines.
Breast Cancer Res. Treat. 54:173-182(1999)

PubMed=10604729; DOI=10.1038/sj.bjc.6695007; PMCID=PMC2362964
Farahnaz Forozan, Robin Veldman, Cheryl A. Ammerman, Nasser Z. Parsa, Anne H. Kallioniemi, Olli-Pekka Kallioniemi, Stephen P. Ethier;
Molecular cytogenetic analysis of 11 new breast cancer cell lines.
Br. J. Cancer 81:1328-1334(1999)

PubMed=10700188; DOI=10.1038/73536
Simon Andrew Gayther, Sarah J. Batley, Lori Linger, Andrew J. Bannister, Karen Thorpe, Suet-Feung Chin, Yataro Daigo, Paul Russell, Annie Wilson, Heidi M. Sowter, Joy Dorothy Ann Delhanty ...Show all 14 authors... , Bruce Anthony John Ponder, Tony Kouzarides, Carlos Caldas; Show fewer authors
Mutations truncating the EP300 acetylase in human cancers.
Nat. Genet. 24:300-303(2000)

PubMed=10969801
Farahnaz Forozan, Eija H. Mahlamaki, Outi Monni, Yi-Dong Chen, Robin Veldman, Yuan Jiang, Gerald C. Gooden, Stephen P. Ethier, Anne H. Kallioniemi, Olli-Pekka Kallioniemi;
Comparative genomic hybridization analysis of 38 breast cancer cell lines: a basis for interpreting complementary DNA microarray data.
Cancer Res. 60:4519-4525(2000)

PubMed=12353263; DOI=10.1002/gcc.10107
Cornel Popovici, Celine Basset, Francois Bertucci, Beatrice Orsetti, Jose Adelaide, Marie-Joelle Mozziconacci, Nathalie Conte, Anne Murati, Christophe Ginestier, Emmanuelle Charafe-Jauffret, Stephen P. Ethier ...Show all 15 authors... , Marina Lafage-Pochitaloff, Charles Theillet, Daniel Birnbaum, Max Chaffanet; Show fewer authors
Reciprocal translocations in breast tumor cell lines: cloning of a t(3;20) that targets the FHIT gene.
Genes Chromosomes Cancer 35:204-218(2002)

PubMed=12800145; DOI=10.1002/gcc.10218
Jose Adelaide, Huai-En Huang, Anne Murati, Amber E. Alsop, Beatrice Orsetti, Marie-Joelle Mozziconacci, Cornel Popovici, Christophe Ginestier, Anne Letessier, Celine Basset, Celine Courtay-Cahen ...Show all 16 authors... , Jocelyne Jacquemier, Charles Theillet, Daniel Birnbaum, Paul A.W. Edwards, Max Chaffanet; Show fewer authors
A recurrent chromosome translocation breakpoint in breast and pancreatic cancer cell lines targets the neuregulin/NRG1 gene.
Genes Chromosomes Cancer 37:333-345(2003)

PubMed=15677628; DOI=10.1093/carcin/bgi032
Kylie Louise Gorringe, Suet-Feung Chin, Paul D.P. Pharoah, Joanne M. Staines, Carla Oliveira, Paul A.W. Edwards, Carlos Caldas;
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)

PubMed=15857504; DOI=10.1186/1475-2867-5-11; PMCID=PMC1090601
Michaela R. Hoffmeyer, Kristin M. Wall, Suranganie F. Dharmawardhane;
In vitro analysis of the invasive phenotype of SUM 149, an inflammatory breast cancer cell line.
Cancer Cell Int. 5:11.1-11.10(2005)

PubMed=16397213; DOI=10.1158/0008-5472.CAN-05-2853
Fons Elstrodt, Antoinette Hollestelle, Jord H.A. Nagel, Michael Gorin, Marijke Wasielewski, Ans M.W. van den Ouweland, Sofia Diana Merajver, Stephen P. Ethier, Mieke Schutte;
BRCA1 mutation analysis of 41 human breast cancer cell lines reveals three new deleterious mutants.
Cancer Res. 66:41-45(2006)

PubMed=16541312; DOI=10.1007/s10549-006-9186-z
Marijke Wasielewski, Fons Elstrodt, Jan G.M. Klijn, Els M.J.J. Berns, Mieke Schutte;
Thirteen new p53 gene mutants identified among 41 human breast cancer cell lines.
Breast Cancer Res. Treat. 99:97-101(2006)

PubMed=17157791; DOI=10.1016/j.ccr.2006.10.008; PMCID=PMC2730521
Richard M. Neve, Koei Chin, Jane Fridlyand, Jennifer Yeh, Frederick L. Baehner, Tea Fevr, Laura Clark, Nora Bayani, Jean-Philippe Coppe, Frances Tong, Terry Speed ...Show all 27 authors... , Paul T. Spellman, Sandy DeVries, Anna Lapuk, Nick J. Wang, Wen-Lin Kuo, Jackie L. Stilwell, Daniel Pinkel, Donna G. Albertson, Frederic M. Waldman, Frank McCormick, Robert Brent Dickson, Michael D. Johnson, Marc E. Lippman, Stephen P. Ethier, Adi F. Gazdar, Joe W. Gray; Show fewer authors
A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes.
Cancer Cell 10:515-527(2006)

PubMed=19582160; DOI=10.1371/journal.pone.0006146; PMCID=PMC2702084
Jessica Kao, Keyan Salari, Melanie Bocanegra, Yoon-La Choi, Luc Girard, Jeet Gandhi, Kevin A. Kwei, Tina Hernandez-Boussard, Pei Wang, Adi F. Gazdar, John D. Minna, Jonathan R. Pollack;
Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery.
PLoS ONE 4:e6146.1-e6146.16(2009)

PubMed=19593635; DOI=10.1007/s10549-009-0460-8
Antoinette Hollestelle, Jord H.A. Nagel, Marcel Smid, Suzanne Lam, Fons Elstrodt, Marijke Wasielewski, Ser Sue Ng, Pim J. French, Justine K. Peeters, Marieke J. Rozendaal, Muhammad Riaz ...Show all 26 authors... , Daphne G. Koopman, Timo L.M. ten Hagen, Bertie H.C.G.M. de Leeuw, Ellen C. Zwarthoff, Amina F.A.S. Teunisse, Peter J. van der Spek, Jan G.M. Klijn, Winand N.M. Dinjens, Stephen P. Ethier, Hans Cornelius Clevers, Aart G. Jochemsen, Michael A. den Bakker, John A. Foekens, John W.M. Martens, Mieke Schutte; Show fewer authors
Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines.
Breast Cancer Res. Treat. 121:53-64(2010)

PubMed=22032724; DOI=10.1186/1755-8794-4-75; PMCID=PMC3227591
Kevin C.H. Ha, Emilie Lalonde, Li-Li Li, Luca Cavallone, Rachael Natrajan, Maryou B. Lambros, Costas Mitsopoulos, Jarle Hakas, Iwanka Kozarewa, Kerry Fenwick, Christopher J. Lord ...Show all 17 authors... , Alan Ashworth, Anne Vincent-Salomon, Mark Basik, Jorge Sergio Reis-Filho, Jacek Majewski, William D. Foulkes; Show fewer authors
Identification of gene fusion transcripts by transcriptome sequencing in BRCA1-mutated breast cancers and cell lines.
BMC Med. Genomics 4:75.1-75.13(2011)

DOI=10.4172/2161-0681.1000119
Fredika M. Robertson, Khoi Chu, Sandra V. Fernandez, Zhao-Mei Mu, Xue-Jun Zhang, Hui Liu, Kimberly M. Boley, R. Katherine Alpaugh, Zai-Ming Ye, Moishia C. Wright, Annie Z. Luo ...Show all 17 authors... , Ricardo Oraes, Hong Wu, Matthew Brent Zook, Sanford H. Barsky, Savitri Krishnamurthy, Massimo Cristofanilli; Show fewer authors
Genomic profiling of pre-clinical models of inflammatory breast cancer identifies a signature of epithelial plasticity and suppression of TGFbeta signaling.
J. Clin. Exp. Pathol. 2:119.1-119.11(2012)

PubMed=23151021; DOI=10.1186/1471-2164-13-619; PMCID=PMC3546428
Anita Grigoriadis, Alan Mackay, Elodie Noel, Pei-Jun Wu, Rachael Natrajan, Jessica Frankum, Jorge Sergio Reis-Filho, Andrew Tutt;
Molecular characterisation of cell line models for triple-negative breast cancers.
BMC Genomics 13:619.1-619.14(2012)

PubMed=23401782; DOI=10.1155/2013/872743; PMCID=PMC3562669
Nandita Barnabas, Dalia Cohen;
Phenotypic and molecular characterization of MCF10DCIS and SUM breast cancer cell lines.
Int. J. Breast Cancer 2013:872743.1-872743.16(2013)

PubMed=23601657; DOI=10.1186/bcr3415; PMCID=PMC3672661
Muhammad Riaz, Marijn T.M. van Jaarsveld, Antoinette Hollestelle, Wendy J.C. Prager-van der Smissen, Anouk A.J. Heine, Antonius W.M. Boersma, Jing-Jing Liu, Jean C.A. Helmijr, Bahar Ozturk, Marcel Smid, Erik A.C. Wiemer ...Show all 13 authors... , John A. Foekens, John W.M. Martens; Show fewer authors
miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation-specific miRNAs.
Breast Cancer Res. 15:R33.1-R33.17(2013)

PubMed=23784380; DOI=10.1007/s10549-013-2600-4; PMCID=PMC4273486
Sandra V. Fernandez, Fredika M. Robertson, Jian-Ming Pei, Lucy Doris Aburto-Chumpitaz, Zhao-Mei Mu, Khoi Chu, R. Katherine Alpaugh, Yong Huang, Yu Cao, Zai-Ming Ye, Kathy Q. Cai ...Show all 16 authors... , Kimberly M. Boley, Andres J.P. Klein-Szanto, Karthik Devarajan, Sankar Addya, Massimo Cristofanilli; Show fewer authors
Inflammatory breast cancer (IBC): clues for targeted therapies.
Breast Cancer Res. Treat. 140:23-33(2013)

PubMed=24162158; DOI=10.1007/s10549-013-2743-3; PMCID=PMC3832776
Aleix Prat, Olga Karginova, Joel S. Parker, Cheng Fan, Xia-Ping He, Lisa M. Bixby, J. Chuck Harrell, Erick Roman, Barbara Adamo, Melissa A. Troester, Charles M. Perou;
Characterization of cell lines derived from breast cancers and normal mammary tissues for the study of the intrinsic molecular subtypes.
Breast Cancer Res. Treat. 142:237-255(2013)

PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110; PMCID=PMC3937590
Anneleen Daemen, Obi L. Griffith, Laura M. Heiser, Nicholas J. Wang, Oana M. Enache, Zachary Sanborn, Francois Pepin, Steffen Durinck, James E. Korkola, Malachi Griffith, Joe S. Hur ...Show all 27 authors... , Nam Huh, Jongsuk Chung, Leslie Cope, Mary Jo Fackler, Christopher Benedict Umbricht, Saraswati Sukumar, Pankaj Seth, Vikas P. Sukhatme, Lakshmi R. Jakkula, Yi-Ling Lu, Gordon B. Mills, Raymond J. Cho, Eric A. Collisson, Laura J. van 't Veer, Paul T. Spellman, Joe W. Gray; Show fewer authors
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26495931; DOI=10.1515/hmbci-2015-0024
Juan Carlos Illera, Sara Caceres, Laura Pena, Paloma Jimena de Andres Gamazo, Beatriz Monsalve, Maria J. Illera, Wendy A. Woodward, James M. Reuben, Gema Silvan;
Steroid hormone secretion in inflammatory breast cancer cell lines.
Horm. Mol. Biol. Clin. Investig. 24:137-145(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26735014; DOI=10.1038/nature16508; PMCID=PMC4854653
Shao-Kun Shu, Charles Y. Lin, Hou-Sheng Hansen He, Robert M. Witwicki, Doris Priscilla Tabassum, Justin M. Roberts, Michalina Janiszewska, Sung Jin Huh, Yi Liang, Jeremy Ryan, Ernest Doherty ...Show all 39 authors... , Hisham Mohammed, Hao Guo, Daniel G. Stover, Muhammad B. Ekram, Jonathan Brown, Clive D'Santos, Ian Elliott Krop, Deborah Dillon, Michael R. McKeown, Christopher Ott, Jun Qi, Min Ni, Prakash K. Rao, Melissa Duarte, Shwu-Yuan Wu, Cheng-Ming Chiang, Lars Anders, Richard Allen Young, Eric Winer, Anthony George Letai, William T. Barry, Jason S. Carroll, Henry Long, Myles A. Brown, Xiao-Le Shirley Liu, Clifford A. Meyer, James E. Bradner, Kornelia Polyak; Show fewer authors
Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer.
Nature 529:413-417(2016)

PubMed=28287265; DOI=10.1021/acs.jproteome.6b00470; PMCID=PMC5557415
Ten-Yang Yen, Spencer Bowen, Roger Yen, Alexandra Piryatinska, Bruce A. Macher, Leslie C. Timpe;
Glycoproteins in claudin-low breast cancer cell lines have a unique expression profile.
J. Proteome Res. 16:1391-1400(2017)

PubMed=32416067; DOI=10.1016/j.molcel.2020.04.027; PMCID=PMC7306005
Shao-Kun Shu, Hua-Jun Wu, Jennifer Y. Ge, Rhamy Zeid, Isaac S. Harris, Bojana Jovanovic, Katherine Murphy, Bin-Bin Wang, Xin-Tao Qiu, Jennifer E. Endress, Jaime Reyes ...Show all 39 authors... , Klothilda Lim, Alba Font-Tello, Sudeepa Syamala, Teng-Fei Xiao, Chandra Sekhar Reddy Chilamakuri, Evangelia K. Papachristou, Clive D'Santos, Jayati Anand, Kunihiko Hinohara, Wei Li, Thomas O. McDonald, Adrienne Luoma, Rebecca J. Modiste, Quang-De Nguyen, Brittany Michel, Paloma Cejas, Cigall Kadoch, Jacob D. Jaffe, Kai W. Wucherpfennig, Jun Qi, Xiao-Le Shirley Liu, Henry Long, Myles A. Brown, Jason S. Carroll, Joan Siefert Brugge, James E. Bradner, Franziska Michor, Kornelia Polyak; Show fewer authors
Synthetic lethal and resistance interactions with BET bromodomain inhibitors in triple-negative breast cancer.
Mol. Cell 78:1096-1113.e8(2020)

PubMed=32715085; DOI=10.1038/s41523-020-0173-z; PMCID=PMC7374090
Stephen P. Ethier, Stephen T. Guest, Elizabeth Garrett-Mayer, Kent Armeson, Robert C. Wilson, Kathryn Duchinski, Daniel Couch, Joe W. Gray, Christiana S. Kappler;
Development and implementation of the SUM breast cancer cell line functional genomics knowledge base.
NPJ Breast Cancer 6:30.1-30.14(2020)

PubMed=35042871; DOI=10.1038/s41523-021-00379-6; PMCID=PMC8766434
Charlotte Rypens, Francois Bertucci, Pascal Finetti, Fredika M. Robertson, Sandra V. Fernandez, Naoto Tada Ueno, Wendy A. Woodward, Kenneth Van Golen, Peter Vermeulen, Luc Dirix, Patrice Viens ...Show all 15 authors... , Daniel Birnbaum, Gayathri R. Devi, Massimo Cristofanilli, Steven Van Laere; Show fewer authors
Comparative transcriptional analyses of preclinical models and patient samples reveal MYC and RELA driven expression patterns that define the molecular landscape of IBC.
NPJ Breast Cancer 8:12.1-12.12(2022)

Cross-references
Cell line collections (Providers) CLS; 300609
Cell line databases/resources CLO; CLO_0009917
cancercelllines; CVCL_3422
Cell_Model_Passport; SIDM01441
DepMap; ACH-001390
LINCS_HMS; 51082
LINCS_LDP; LCL-2067
SLKBase; 249
Anatomy/cell type resources BTO; BTO_0005475
Biological sample resources BioSample; SAMN03472959
CRISP screens repositories BioGRID_ORCS_Cell_line; 1311
Chemistry resources ChEMBL-Cells; CHEMBL4295448
ChEMBL-Targets; CHEMBL4296497
PharmacoDB; SUM149PT_1509_2019
PubChem_Cell_line; CVCL_3422
Encyclopedic resources Wikidata; Q54970832
Experimental variables resources EFO; EFO_0001240
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-11134
ArrayExpress; E-TABM-157
GEO; GSM115105
GEO; GSM217600
GEO; GSM350549
GEO; GSM421889
GEO; GSM481335
GEO; GSM501233
GEO; GSM501234
GEO; GSM501235
GEO; GSM501237
GEO; GSM501238
GEO; GSM844705
GEO; GSM847433
GEO; GSM847500
GEO; GSM1008916
GEO; GSM1053736
GEO; GSM1172897
GEO; GSM1172992
GEO; GSM1214568
GEO; GSM1215280
GEO; GSM1238140
GEO; GSM1374911
GEO; GSM1401669
GEO; GSM3145734
Polymorphism and mutation databases Cosmic; 1044208
Cosmic; 1046959
Cosmic; 1136379
Cosmic; 1176631
Cosmic; 1287929
Cosmic; 1289411
Cosmic; 1460239
Cosmic; 1523966
Cosmic; 1603224
Cosmic; 2036674
IARC_TP53; 24356
Progenetix; CVCL_3422
Proteomic databases PRIDE; PXD005295
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001002554
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number41