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Cellosaurus Rj2.2.5 (CVCL_3414)

[Text version]
Cell line name Rj2.2.5
Synonyms RJ2.2.5; RJ-2.2.5; RJ 2.2.5; RJ2.25; Raji 2.2.5
Accession CVCL_3414
Resource Identification Initiative To cite this cell line use: Rj2.2.5 (RRID:CVCL_3414)
Comments Population: African; Nigerian.
Characteristics: Deficient in expression of HLA class II antigens.
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Transformant: NCIt; C44386; Gamma radiation.
Omics: H3K27ac ChIP-seq epigenome analysis.
Derived from site: In situ; Bone, jaw, maxilla; UBERON=UBERON_0002397.
Sequence variations
  • Gene deletion; HGNC; 7067; CIITA; Zygosity=Heterozygous (PubMed=8402893).
  • Gene fusion; HGNC; 5477; IGH + HGNC; 7553; MYC; Name(s)=MYC-IGH (from parent cell line).
  • Mutation; HGNC; 7067; CIITA; Unexplicit; Major internal deletion; Zygosity=Heterozygous (PubMed=8402893).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg213Gln (c.638G>A); ClinVar=VCV000135359; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Tyr234His (c.700T>C); ClinVar=VCV000376691; Zygosity=Heterozygous (from parent cell line).
HLA typing Source: CLS=300360
Class I
HLA-AA*03:01:01
HLA-BB*15:10:01
HLA-CC*03:04:02,04:01:01
Class II
HLA-DPDPB1*01:01:01
HLA-DQDQA1*01:05:01,05:01:01
DQB1*02:01:01,05:01:01
HLA-DRDRB1*03:01:01,10:01:01
Disease EBV-related Burkitt lymphoma (NCIt: C27694)
Burkitt lymphoma (ORDO: Orphanet_543)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0511 (Raji)
Sex of cell Male
Age at sampling 11Y
Category Cancer cell line
STR profile Source(s): CLS

Markers:
AmelogeninX,Y
CSF1PO10,12
D3S135815,16
D5S81810
D7S82010
D8S117914,15
D13S31713
D16S5398,11
D18S5117
D21S1128,31
FGA19,27
Penta D3.2,9
Penta E5,13
TH016,7
TPOX8,13
vWA16,19

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/r/cell-lines-detail-28.html
https://emoryott.technologypublisher.com/tech/CIITA_Deficient_Human_B_Cell_Line
Publications

PubMed=6403646; DOI=10.1084/jem.157.3.1053
Accolla R.S.
Human B cell variants immunoselected against a single Ia antigen subset have lost expression of several Ia antigen subsets.
J. Exp. Med. 157:1053-1058(1983)

PubMed=2511169; DOI=10.1016/0198-8859(89)90007-4
Hume C.R., Lee J.S.
Congenital immunodeficiencies associated with absence of HLA class II antigens on lymphocytes result from distinct mutations in trans-acting factors.
Hum. Immunol. 26:288-309(1989)

PubMed=1852002; DOI=10.1073/pnas.88.10.4285
Benichou B., Strominger J.L.
Class II-antigen-negative patient and mutant B-cell lines represent at least three, and probably four, distinct genetic defects defined by complementation analysis.
Proc. Natl. Acad. Sci. U.S.A. 88:4285-4288(1991)

PubMed=1538137; DOI=10.4049/jimmunol.148.5.1576
Seidl C., Saraiya C., Osterweil Z., Fu Y.P., Lee J.S.
Genetic complexity of regulatory mutants defective for HLA class II gene expression.
J. Immunol. 148:1576-1584(1992)

PubMed=8402893; DOI=10.1016/S0092-8674(05)80090-X
Steimle V., Otten L.A., Zufferey M., Mach B.
Complementation cloning of an MHC class II transactivator mutated in hereditary MHC class II deficiency (or bare lymphocyte syndrome).
Cell 75:135-146(1993)

PubMed=8441650; DOI=10.1093/nar/21.3.381
Ombra M.N., Perfetto C., Autiero M., Anzisi A.M., Pasquinelli R., Maffei A., Del Pozzo G., Guardiola J.
Reversion of a transcriptionally defective MHC class II-negative human B-cell mutant.
Nucleic Acids Res. 21:381-386(1993)

Cross-references
Cell line collections (Providers) CLS; 300360
ICLC; HTL02002
Cell line databases/resources CLDB; cl7125
cancercelllines; CVCL_3414
Encyclopedic resources Wikidata; Q54950516
Gene expression databases GEO; GSM1602239
GEO; GSM1602240
GEO; GSM1602245
GEO; GSM1602246
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number23